The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent

Allergens are initiators of both innate and adaptive immune responses. They are recognised at the site of entry by epithelial and dendritic cells (DCs), both of which activate innate inflammatory circuits that can collectively induce Th2 immune responses. In an attempt to have a better understanding...

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Main Authors:	Al-Ghouleh, Abeer, Johal, Ramneek, Sharquie, Inas K., Emara, Mohamed, Harrington, Helen, Shakib, Farouk, Ghaemmaghami, Amir M.
Format:	Article
Published:	Public Library of Science 2012
Online Access:	https://eprints.nottingham.ac.uk/2922/

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author	Al-Ghouleh, Abeer Johal, Ramneek Sharquie, Inas K. Emara, Mohamed Harrington, Helen Shakib, Farouk Ghaemmaghami, Amir M.
author_facet	Al-Ghouleh, Abeer Johal, Ramneek Sharquie, Inas K. Emara, Mohamed Harrington, Helen Shakib, Farouk Ghaemmaghami, Amir M.
author_sort	Al-Ghouleh, Abeer
building	Nottingham Research Data Repository
collection	Online Access
description	Allergens are initiators of both innate and adaptive immune responses. They are recognised at the site of entry by epithelial and dendritic cells (DCs), both of which activate innate inflammatory circuits that can collectively induce Th2 immune responses. In an attempt to have a better understanding of the role of carbohydrates in the recognition and uptake of allergens by the innate immune system, we defined common glycosylation patterns in major allergens. This was done using labelled lectins and showed that allergens like Der p 1 (Dermatophagoides pteronyssinus group 1), Fel d 1 (Felis domisticus), Ara h 1 (Arachis hypogaea), Der p 2 (Dermatophagoides pteronyssinus group 2), Bla g 2 (Blattella germanica) and Can f 1 (Canis familiaris) are glycosylated and that the main dominant sugars on these allergens are 1–2, 1–3 and 1–6 mannose. These observations are in line with recent reports implicating the mannose receptor (MR) in allergen recognition and uptake by DCs and suggesting a major link between glycosylation and allergen recognition. We then looked at TSLP (Thymic Stromal Lymphopoietin) cytokine secretion by lung epithelia upon encountering natural Der p 1 allergen. TSLP is suggested to drive DC maturation in support of allergic hypersensitivity reactions. Our data showed an increase in TSLP secretion by lung epithelia upon stimulation with natural Der p 1 which was carbohydrate dependent. The deglycosylated preparation of Der p 1 exhibited minimal uptake by DCs compared to the natural and hyperglycosylated recombinant counterparts, with the latter being taken up more readily than the other preparations. Collectively, our data indicate that carbohydrate moieties on allergens play a vital role in their recognition by innate immune cells, implicating them in downstream deleterious Th2 cell activation and IgE production.
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format	Article
id	nottingham-2922
institution	University of Nottingham Malaysia Campus
institution_category	Local University
last_indexed	2025-11-14T18:20:06Z
publishDate	2012
publisher	Public Library of Science
recordtype	eprints
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spelling	nottingham-29222020-05-04T16:32:42Z https://eprints.nottingham.ac.uk/2922/ The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent Al-Ghouleh, Abeer Johal, Ramneek Sharquie, Inas K. Emara, Mohamed Harrington, Helen Shakib, Farouk Ghaemmaghami, Amir M. Allergens are initiators of both innate and adaptive immune responses. They are recognised at the site of entry by epithelial and dendritic cells (DCs), both of which activate innate inflammatory circuits that can collectively induce Th2 immune responses. In an attempt to have a better understanding of the role of carbohydrates in the recognition and uptake of allergens by the innate immune system, we defined common glycosylation patterns in major allergens. This was done using labelled lectins and showed that allergens like Der p 1 (Dermatophagoides pteronyssinus group 1), Fel d 1 (Felis domisticus), Ara h 1 (Arachis hypogaea), Der p 2 (Dermatophagoides pteronyssinus group 2), Bla g 2 (Blattella germanica) and Can f 1 (Canis familiaris) are glycosylated and that the main dominant sugars on these allergens are 1–2, 1–3 and 1–6 mannose. These observations are in line with recent reports implicating the mannose receptor (MR) in allergen recognition and uptake by DCs and suggesting a major link between glycosylation and allergen recognition. We then looked at TSLP (Thymic Stromal Lymphopoietin) cytokine secretion by lung epithelia upon encountering natural Der p 1 allergen. TSLP is suggested to drive DC maturation in support of allergic hypersensitivity reactions. Our data showed an increase in TSLP secretion by lung epithelia upon stimulation with natural Der p 1 which was carbohydrate dependent. The deglycosylated preparation of Der p 1 exhibited minimal uptake by DCs compared to the natural and hyperglycosylated recombinant counterparts, with the latter being taken up more readily than the other preparations. Collectively, our data indicate that carbohydrate moieties on allergens play a vital role in their recognition by innate immune cells, implicating them in downstream deleterious Th2 cell activation and IgE production. Public Library of Science 2012-03-30 Article PeerReviewed Al-Ghouleh, Abeer, Johal, Ramneek, Sharquie, Inas K., Emara, Mohamed, Harrington, Helen, Shakib, Farouk and Ghaemmaghami, Amir M. (2012) The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent. PLoS ONE, 7 (3). 11/1-11/11. ISSN 1932-6203 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0033929 doi:10.1371/journal.pone.0033929 doi:10.1371/journal.pone.0033929
spellingShingle	Al-Ghouleh, Abeer Johal, Ramneek Sharquie, Inas K. Emara, Mohamed Harrington, Helen Shakib, Farouk Ghaemmaghami, Amir M. The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent
title	The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent
title_full	The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent
title_fullStr	The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent
title_full_unstemmed	The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent
title_short	The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent
title_sort	glycosylation pattern of common allergens: the recognition and uptake of der p 1 by epithelial and dendritic cells is carbohydrate dependent
url	https://eprints.nottingham.ac.uk/2922/ https://eprints.nottingham.ac.uk/2922/ https://eprints.nottingham.ac.uk/2922/

The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent

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