Assessment of the anthelmintic effect of natural plant cysteine proteinases against the gastrointestinal nematode, Heligmosomoides polygyrus, in vitro
We examined the mechanism of action and compared the anthelmintic efficacy of cysteine proteinases from papaya, pineapple, fig, kiwi fruit and Egyptian milkweed in vitro using the rodent gastrointestinal nematode Heligmosomoides polygyrus. Within a 2 h incubation period, all the cysteine proteinases...
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Cambridge University Press
2005
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| Online Access: | https://eprints.nottingham.ac.uk/29203/ |
| _version_ | 1848793736250654720 |
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| author | Stepek, Gillian Buttle, David J. Duce, I.R. Lowe, Ann Behnke, Jerzy M. |
| author_facet | Stepek, Gillian Buttle, David J. Duce, I.R. Lowe, Ann Behnke, Jerzy M. |
| author_sort | Stepek, Gillian |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | We examined the mechanism of action and compared the anthelmintic efficacy of cysteine proteinases from papaya, pineapple, fig, kiwi fruit and Egyptian milkweed in vitro using the rodent gastrointestinal nematode Heligmosomoides polygyrus. Within a 2 h incubation period, all the cysteine proteinases, with the exception of the kiwi fruit extract, caused marked damage to the cuticle of H. polygyrus adult male and female worms, reflected in the loss of surface cuticular layers. Efficacy was comparable for both sexes of worms, was dependent on the presence of cysteine and was completely inhibited by the cysteine proteinase inhibitor, E-64. LD50 values indicated that the purified proteinases were more efficacious than the proteinases in the crude latex, with purified ficin, papain, chymopapain, Egyptian milkweed latex extract and pineapple fruit extract, containing fruit bromelain, having the most potent effect. The mechanism of action of these plant enzymes (i.e. an attack on the protective cuticle of the worm) suggests that resistance would be slow to develop in the field. The efficacy and mode of action make plant cysteine proteinases potential candidates for a novel class of anthelmintics urgently required for the treatment of humans and domestic livestock. |
| first_indexed | 2025-11-14T19:05:02Z |
| format | Article |
| id | nottingham-29203 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:05:02Z |
| publishDate | 2005 |
| publisher | Cambridge University Press |
| recordtype | eprints |
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| spelling | nottingham-292032020-05-04T20:31:00Z https://eprints.nottingham.ac.uk/29203/ Assessment of the anthelmintic effect of natural plant cysteine proteinases against the gastrointestinal nematode, Heligmosomoides polygyrus, in vitro Stepek, Gillian Buttle, David J. Duce, I.R. Lowe, Ann Behnke, Jerzy M. We examined the mechanism of action and compared the anthelmintic efficacy of cysteine proteinases from papaya, pineapple, fig, kiwi fruit and Egyptian milkweed in vitro using the rodent gastrointestinal nematode Heligmosomoides polygyrus. Within a 2 h incubation period, all the cysteine proteinases, with the exception of the kiwi fruit extract, caused marked damage to the cuticle of H. polygyrus adult male and female worms, reflected in the loss of surface cuticular layers. Efficacy was comparable for both sexes of worms, was dependent on the presence of cysteine and was completely inhibited by the cysteine proteinase inhibitor, E-64. LD50 values indicated that the purified proteinases were more efficacious than the proteinases in the crude latex, with purified ficin, papain, chymopapain, Egyptian milkweed latex extract and pineapple fruit extract, containing fruit bromelain, having the most potent effect. The mechanism of action of these plant enzymes (i.e. an attack on the protective cuticle of the worm) suggests that resistance would be slow to develop in the field. The efficacy and mode of action make plant cysteine proteinases potential candidates for a novel class of anthelmintics urgently required for the treatment of humans and domestic livestock. Cambridge University Press 2005 Article PeerReviewed Stepek, Gillian, Buttle, David J., Duce, I.R., Lowe, Ann and Behnke, Jerzy M. (2005) Assessment of the anthelmintic effect of natural plant cysteine proteinases against the gastrointestinal nematode, Heligmosomoides polygyrus, in vitro. Parasitology, 130 (2). pp. 203-211. ISSN 0031-1820 Plant cysteine proteinases gastrointestinal nematodes anthelmintic Heligmosomoides polygyrus. http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=279002&fileId=S0031182004006225 doi:10.1017/S0031182004006225 doi:10.1017/S0031182004006225 |
| spellingShingle | Plant cysteine proteinases gastrointestinal nematodes anthelmintic Heligmosomoides polygyrus. Stepek, Gillian Buttle, David J. Duce, I.R. Lowe, Ann Behnke, Jerzy M. Assessment of the anthelmintic effect of natural plant cysteine proteinases against the gastrointestinal nematode, Heligmosomoides polygyrus, in vitro |
| title | Assessment of the anthelmintic effect of natural plant
cysteine proteinases against the gastrointestinal
nematode, Heligmosomoides polygyrus, in vitro |
| title_full | Assessment of the anthelmintic effect of natural plant
cysteine proteinases against the gastrointestinal
nematode, Heligmosomoides polygyrus, in vitro |
| title_fullStr | Assessment of the anthelmintic effect of natural plant
cysteine proteinases against the gastrointestinal
nematode, Heligmosomoides polygyrus, in vitro |
| title_full_unstemmed | Assessment of the anthelmintic effect of natural plant
cysteine proteinases against the gastrointestinal
nematode, Heligmosomoides polygyrus, in vitro |
| title_short | Assessment of the anthelmintic effect of natural plant
cysteine proteinases against the gastrointestinal
nematode, Heligmosomoides polygyrus, in vitro |
| title_sort | assessment of the anthelmintic effect of natural plant
cysteine proteinases against the gastrointestinal
nematode, heligmosomoides polygyrus, in vitro |
| topic | Plant cysteine proteinases gastrointestinal nematodes anthelmintic Heligmosomoides polygyrus. |
| url | https://eprints.nottingham.ac.uk/29203/ https://eprints.nottingham.ac.uk/29203/ https://eprints.nottingham.ac.uk/29203/ |