Optimisation and use of humanised RBL NF-AT-GFP and NF-AT-DsRed reporter cell lines suitable for high-throughput scale detection of allergic sensitisation in array format and identification of the ECM–Integrin interaction as critical factor

Optimisation and use of humanised RBL NF-AT-GFP and NF-AT-DsRed reporter cell lines suitable for high-throughput scale detection of allergic sensitisation in array format and identification of the ECM–Integrin interaction as critical factor

We have previously described a microarray platform combining live basophils with protein arrays suitable for high-throughput detection of sensitisation against allergens. During optimisation of this technique, we observed severe losses of adhering cells during the washing steps, particularly after a...

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Bibliographic Details
Main Authors: Wang, Xiaowei, Cato, Paul, Lin, Hsiu-Chen, Li, Tongen, Wan, Daniel, Alcocer, Marcos J.C., Falcone, Franco H.
Format: Article
Published: Humana Press 2014
Subjects:
RBL
Basophil array
Adhesion
Fibronectin
VLA-4
Reporter system
GFP
DsRed Intro
Online Access:https://eprints.nottingham.ac.uk/2916/

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