Nanoparticle transport in epithelial cells: pathway switching through bioconjugation
The understanding and control of nanoparticle transport into and through cellular compartments is central to biomedical applications of nanotechnology. Here, it is shown that the transport pathway of 50 nm polystyrene nanoparticles decorated with vitamin B12 in epithelial cells is different compared...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
Wiley-VCH Verlag
2013
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/2915/ |
| _version_ | 1848790908009447424 |
|---|---|
| author | Fowler, Robyn Vllasaliu, Driton Trillo, Francisco Fernández Garnett, Martin Alexander, Cameron Horsley, Helen Smith, Bryan Whitcombe, Ian Eaton, Mike Stolnik, Snow |
| author_facet | Fowler, Robyn Vllasaliu, Driton Trillo, Francisco Fernández Garnett, Martin Alexander, Cameron Horsley, Helen Smith, Bryan Whitcombe, Ian Eaton, Mike Stolnik, Snow |
| author_sort | Fowler, Robyn |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The understanding and control of nanoparticle transport into and through cellular compartments is central to biomedical applications of nanotechnology. Here, it is shown that the transport pathway of 50 nm polystyrene nanoparticles decorated with vitamin B12 in epithelial cells is different compared to both soluble B12 ligand and unmodified nanoparticles, and this is not attributable to B12 recognition alone. Importantly, the study indicates that vitamin B12-conjugated nanoparticles circumnavigate the lysosomal compartment, the destination of soluble vitamin B12 ligand. Whereas cellular trafficking of soluble B12 is confirmed to occur via the clathrin-mediated pathway, transport of B12-conjugated nanoparticles appears to predominantly take place by a route that is perturbed by caveolae-specific inhibitors. This data suggests that, following its conjugation to nanoparticles, in addition to dramatically increasing the cellular uptake of nanoparticles, the normal cell trafficking of B12 is switched to an alternative pathway, omitting the lysosomal stage: a result with important implications for oral delivery of nanoparticulate diagnostics and therapeutics. |
| first_indexed | 2025-11-14T18:20:05Z |
| format | Article |
| id | nottingham-2915 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:20:05Z |
| publishDate | 2013 |
| publisher | Wiley-VCH Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-29152020-05-04T16:39:28Z https://eprints.nottingham.ac.uk/2915/ Nanoparticle transport in epithelial cells: pathway switching through bioconjugation Fowler, Robyn Vllasaliu, Driton Trillo, Francisco Fernández Garnett, Martin Alexander, Cameron Horsley, Helen Smith, Bryan Whitcombe, Ian Eaton, Mike Stolnik, Snow The understanding and control of nanoparticle transport into and through cellular compartments is central to biomedical applications of nanotechnology. Here, it is shown that the transport pathway of 50 nm polystyrene nanoparticles decorated with vitamin B12 in epithelial cells is different compared to both soluble B12 ligand and unmodified nanoparticles, and this is not attributable to B12 recognition alone. Importantly, the study indicates that vitamin B12-conjugated nanoparticles circumnavigate the lysosomal compartment, the destination of soluble vitamin B12 ligand. Whereas cellular trafficking of soluble B12 is confirmed to occur via the clathrin-mediated pathway, transport of B12-conjugated nanoparticles appears to predominantly take place by a route that is perturbed by caveolae-specific inhibitors. This data suggests that, following its conjugation to nanoparticles, in addition to dramatically increasing the cellular uptake of nanoparticles, the normal cell trafficking of B12 is switched to an alternative pathway, omitting the lysosomal stage: a result with important implications for oral delivery of nanoparticulate diagnostics and therapeutics. Wiley-VCH Verlag 2013-10-04 Article PeerReviewed Fowler, Robyn, Vllasaliu, Driton, Trillo, Francisco Fernández, Garnett, Martin, Alexander, Cameron, Horsley, Helen, Smith, Bryan, Whitcombe, Ian, Eaton, Mike and Stolnik, Snow (2013) Nanoparticle transport in epithelial cells: pathway switching through bioconjugation. Small, 9 (19). pp. 3282-3294. ISSN 1613-6829 Caco-2 cells; drug delivery; endocytosis pathways; nanoparticles; vitamin B12 http://onlinelibrary.wiley.com/doi/10.1002/smll.201202623/abstract;jsessionid=CABFF56A9C453DA133CAD70168F16CD5.f01t04 doi:10.1002/smll.201202623 doi:10.1002/smll.201202623 |
| spellingShingle | Caco-2 cells; drug delivery; endocytosis pathways; nanoparticles; vitamin B12 Fowler, Robyn Vllasaliu, Driton Trillo, Francisco Fernández Garnett, Martin Alexander, Cameron Horsley, Helen Smith, Bryan Whitcombe, Ian Eaton, Mike Stolnik, Snow Nanoparticle transport in epithelial cells: pathway switching through bioconjugation |
| title | Nanoparticle transport in epithelial cells: pathway switching through bioconjugation |
| title_full | Nanoparticle transport in epithelial cells: pathway switching through bioconjugation |
| title_fullStr | Nanoparticle transport in epithelial cells: pathway switching through bioconjugation |
| title_full_unstemmed | Nanoparticle transport in epithelial cells: pathway switching through bioconjugation |
| title_short | Nanoparticle transport in epithelial cells: pathway switching through bioconjugation |
| title_sort | nanoparticle transport in epithelial cells: pathway switching through bioconjugation |
| topic | Caco-2 cells; drug delivery; endocytosis pathways; nanoparticles; vitamin B12 |
| url | https://eprints.nottingham.ac.uk/2915/ https://eprints.nottingham.ac.uk/2915/ https://eprints.nottingham.ac.uk/2915/ |