Filamentation of Campylobacter in broth cultures
The transition from rod to filamentous cell morphology has been identified as a response to stressful conditions in many bacterial species and has been ascribed to confer certain survival advantages. Filamentation of Campylobacter jejuni was demonstrated to occur spontaneously on entry in to station...
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Frontiers
2015
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| Online Access: | https://eprints.nottingham.ac.uk/29132/ |
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| author | Ghaffar, Nacheervan M. Connerton, Phillippa L. Connerton, Ian F. |
| author_facet | Ghaffar, Nacheervan M. Connerton, Phillippa L. Connerton, Ian F. |
| author_sort | Ghaffar, Nacheervan M. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The transition from rod to filamentous cell morphology has been identified as a response to stressful conditions in many bacterial species and has been ascribed to confer certain survival advantages. Filamentation of Campylobacter jejuni was demonstrated to occur spontaneously on entry in to stationary phase distinguishing it from many other bacteria where a reduction in size is more common. The aim of this study was to investigate the cues that give rise to filamentation of C. jejuni and C. coli and gain insights into the process. Using minimal medium, augmentation of filamentation occurred and it was observed that this morphological change was wide spread amongst C. jejuni strains tested but was not universal in C. coli strains. Filamentation did not appear to be due to release of diffusible molecules, toxic metabolites, or be in response to oxidative stress in the medium. Separated filaments exhibited greater intracellular ATP contents (2.66 to 17.4 fg) than spiral forms (0.99 to 1.7 fg) and showed enhanced survival in water at 4oC and 37oC compared to spiral cells. These observations support the conclusion that the filaments are adapted to survive extra-intestinal environments. Differences in cell morphology and physiology need to be considered in the context of the design of experimental studies and the methods adopted for the isolation of campylobacters from food, clinical and environmental sources. |
| first_indexed | 2025-11-14T19:04:48Z |
| format | Article |
| id | nottingham-29132 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:04:48Z |
| publishDate | 2015 |
| publisher | Frontiers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-291322020-05-04T17:09:32Z https://eprints.nottingham.ac.uk/29132/ Filamentation of Campylobacter in broth cultures Ghaffar, Nacheervan M. Connerton, Phillippa L. Connerton, Ian F. The transition from rod to filamentous cell morphology has been identified as a response to stressful conditions in many bacterial species and has been ascribed to confer certain survival advantages. Filamentation of Campylobacter jejuni was demonstrated to occur spontaneously on entry in to stationary phase distinguishing it from many other bacteria where a reduction in size is more common. The aim of this study was to investigate the cues that give rise to filamentation of C. jejuni and C. coli and gain insights into the process. Using minimal medium, augmentation of filamentation occurred and it was observed that this morphological change was wide spread amongst C. jejuni strains tested but was not universal in C. coli strains. Filamentation did not appear to be due to release of diffusible molecules, toxic metabolites, or be in response to oxidative stress in the medium. Separated filaments exhibited greater intracellular ATP contents (2.66 to 17.4 fg) than spiral forms (0.99 to 1.7 fg) and showed enhanced survival in water at 4oC and 37oC compared to spiral cells. These observations support the conclusion that the filaments are adapted to survive extra-intestinal environments. Differences in cell morphology and physiology need to be considered in the context of the design of experimental studies and the methods adopted for the isolation of campylobacters from food, clinical and environmental sources. Frontiers 2015-06-30 Article PeerReviewed Ghaffar, Nacheervan M., Connerton, Phillippa L. and Connerton, Ian F. (2015) Filamentation of Campylobacter in broth cultures. Frontiers in Microbiology, 6 (657). pp. 1-9. ISSN 1664-302X Campylobacter filamentation morphological changes morphotypes survival intracellular ATP http://dx.doi.org/10.3389/fmicb.2015.00657 doi:10.3389/fmicb.2015.00657 doi:10.3389/fmicb.2015.00657 |
| spellingShingle | Campylobacter filamentation morphological changes morphotypes survival intracellular ATP Ghaffar, Nacheervan M. Connerton, Phillippa L. Connerton, Ian F. Filamentation of Campylobacter in broth cultures |
| title | Filamentation of Campylobacter in broth cultures |
| title_full | Filamentation of Campylobacter in broth cultures |
| title_fullStr | Filamentation of Campylobacter in broth cultures |
| title_full_unstemmed | Filamentation of Campylobacter in broth cultures |
| title_short | Filamentation of Campylobacter in broth cultures |
| title_sort | filamentation of campylobacter in broth cultures |
| topic | Campylobacter filamentation morphological changes morphotypes survival intracellular ATP |
| url | https://eprints.nottingham.ac.uk/29132/ https://eprints.nottingham.ac.uk/29132/ https://eprints.nottingham.ac.uk/29132/ |