Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling
The detection and inactivation of pathogenic strains of bacteria continues to be an important therapeutic goal. Hence, there is a need for materials that can bind selectively to specific microorganisms, for diagnostic or anti-infective applications, but which can be formed from simple and inexpensiv...
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| Format: | Article |
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Nature Publishing Group
2014
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| Online Access: | https://eprints.nottingham.ac.uk/28832/ |
| _version_ | 1848793653445656576 |
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| author | Magennis, Eugene Peter Fernandez-Trillo, Francisco Sui, Cheng Spain, Sebastian G. Bradshaw, David Churchley, David Mantovani, Giuseppe Winzer, Klaus Alexander, Cameron |
| author_facet | Magennis, Eugene Peter Fernandez-Trillo, Francisco Sui, Cheng Spain, Sebastian G. Bradshaw, David Churchley, David Mantovani, Giuseppe Winzer, Klaus Alexander, Cameron |
| author_sort | Magennis, Eugene Peter |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The detection and inactivation of pathogenic strains of bacteria continues to be an important therapeutic goal. Hence, there is a need for materials that can bind selectively to specific microorganisms, for diagnostic or anti-infective applications, but which can be formed from simple and inexpensive building blocks. Here, we exploit bacterial redox systems to induce a copper-mediated radical polymerisation of synthetic monomers at cell surfaces, generating polymers in situ that bind strongly to the microorganisms which produced them. This ‘bacteria-instructed synthesis’ can be carried out with a variety of microbial strains, and we show that the polymers produced are self-selective binding agents for the ‘instructing’ cell types. We further expand on the bacterial redox chemistries to ‘click’ fluorescent reporters onto polymers directly at the surfaces of a range of clinical isolate strains, allowing rapid, facile and simultaneous binding and visualisation of pathogens. |
| first_indexed | 2025-11-14T19:03:43Z |
| format | Article |
| id | nottingham-28832 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:03:43Z |
| publishDate | 2014 |
| publisher | Nature Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-288322020-05-04T20:14:01Z https://eprints.nottingham.ac.uk/28832/ Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling Magennis, Eugene Peter Fernandez-Trillo, Francisco Sui, Cheng Spain, Sebastian G. Bradshaw, David Churchley, David Mantovani, Giuseppe Winzer, Klaus Alexander, Cameron The detection and inactivation of pathogenic strains of bacteria continues to be an important therapeutic goal. Hence, there is a need for materials that can bind selectively to specific microorganisms, for diagnostic or anti-infective applications, but which can be formed from simple and inexpensive building blocks. Here, we exploit bacterial redox systems to induce a copper-mediated radical polymerisation of synthetic monomers at cell surfaces, generating polymers in situ that bind strongly to the microorganisms which produced them. This ‘bacteria-instructed synthesis’ can be carried out with a variety of microbial strains, and we show that the polymers produced are self-selective binding agents for the ‘instructing’ cell types. We further expand on the bacterial redox chemistries to ‘click’ fluorescent reporters onto polymers directly at the surfaces of a range of clinical isolate strains, allowing rapid, facile and simultaneous binding and visualisation of pathogens. Nature Publishing Group 2014-07 Article PeerReviewed Magennis, Eugene Peter, Fernandez-Trillo, Francisco, Sui, Cheng, Spain, Sebastian G., Bradshaw, David, Churchley, David, Mantovani, Giuseppe, Winzer, Klaus and Alexander, Cameron (2014) Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling. Nature Materials, 13 (7). pp. 748-755. ISSN 1476-1122 Biomaterials molecular recognition antimicrobial resistance smart polymers bacterial detection http://www.nature.com/nmat/journal/v13/n7/full/nmat3949.html doi:10.1038/nmat3949 doi:10.1038/nmat3949 |
| spellingShingle | Biomaterials molecular recognition antimicrobial resistance smart polymers bacterial detection Magennis, Eugene Peter Fernandez-Trillo, Francisco Sui, Cheng Spain, Sebastian G. Bradshaw, David Churchley, David Mantovani, Giuseppe Winzer, Klaus Alexander, Cameron Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling |
| title | Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling |
| title_full | Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling |
| title_fullStr | Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling |
| title_full_unstemmed | Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling |
| title_short | Bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling |
| title_sort | bacteria-instructed synthesis of polymers for self-selective microbial binding and labelling |
| topic | Biomaterials molecular recognition antimicrobial resistance smart polymers bacterial detection |
| url | https://eprints.nottingham.ac.uk/28832/ https://eprints.nottingham.ac.uk/28832/ https://eprints.nottingham.ac.uk/28832/ |