Microencapsulation of Lactobacillus acidophilus NCIMB 701748 in matrices containing soluble fibre by spray drying: technological characterization, storage stability and survival after in vitro digestion

We evaluated sodium alginate, chitosan and hydroxypropyl methylcellulose (HPMC) as co-encapsulants for spray dried Lactobacillus acidophilus NCIMB 701748 by assessing their impact on cell viability and physicochemical properties of the dried powders, viability over 35 days of storage at 25 °C and su...

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Main Authors: Yonekura, Lina, Sun, Han, Soukoulis, Christos, Fisk, Ian D.
Format: Article
Published: Elsevier 2014
Subjects:
Online Access:https://eprints.nottingham.ac.uk/28790/
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author Yonekura, Lina
Sun, Han
Soukoulis, Christos
Fisk, Ian D.
author_facet Yonekura, Lina
Sun, Han
Soukoulis, Christos
Fisk, Ian D.
author_sort Yonekura, Lina
building Nottingham Research Data Repository
collection Online Access
description We evaluated sodium alginate, chitosan and hydroxypropyl methylcellulose (HPMC) as co-encapsulants for spray dried Lactobacillus acidophilus NCIMB 701748 by assessing their impact on cell viability and physicochemical properties of the dried powders, viability over 35 days of storage at 25 °C and survival after simulated digestion. Fibres were added to a control carrier medium containing whey protein concentrate, d-glucose and maltodextrin. Sodium alginate and HPMC did not affect cell viability but chitosan reduced viable counts in spray dried powders, as compared to the control. Although chitosan caused large losses of viability during spray-drying, these losses were counteracted by the excellent storage stability compared to control, sodium alginate and HPMC, and the overall effect became positive after the 35-day storage. Chitosan also improved survival rates in simulated GI conditions, however no single fibre could improve L. acidophilus NCIMB 701748 viability in all steps from production through storage and digestion.
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spelling nottingham-287902020-05-04T20:15:58Z https://eprints.nottingham.ac.uk/28790/ Microencapsulation of Lactobacillus acidophilus NCIMB 701748 in matrices containing soluble fibre by spray drying: technological characterization, storage stability and survival after in vitro digestion Yonekura, Lina Sun, Han Soukoulis, Christos Fisk, Ian D. We evaluated sodium alginate, chitosan and hydroxypropyl methylcellulose (HPMC) as co-encapsulants for spray dried Lactobacillus acidophilus NCIMB 701748 by assessing their impact on cell viability and physicochemical properties of the dried powders, viability over 35 days of storage at 25 °C and survival after simulated digestion. Fibres were added to a control carrier medium containing whey protein concentrate, d-glucose and maltodextrin. Sodium alginate and HPMC did not affect cell viability but chitosan reduced viable counts in spray dried powders, as compared to the control. Although chitosan caused large losses of viability during spray-drying, these losses were counteracted by the excellent storage stability compared to control, sodium alginate and HPMC, and the overall effect became positive after the 35-day storage. Chitosan also improved survival rates in simulated GI conditions, however no single fibre could improve L. acidophilus NCIMB 701748 viability in all steps from production through storage and digestion. Elsevier 2014-01 Article PeerReviewed Yonekura, Lina, Sun, Han, Soukoulis, Christos and Fisk, Ian D. (2014) Microencapsulation of Lactobacillus acidophilus NCIMB 701748 in matrices containing soluble fibre by spray drying: technological characterization, storage stability and survival after in vitro digestion. Journal of Functional Foods, 6 . pp. 205-214. ISSN 1756-4646 Probiotics; Heat stress; Gastrointestinal tolerance; Sodium alginate; HPMC; Chitosan http://www.sciencedirect.com/science/article/pii/S1756464613002363# doi:10.1016/j.jff.2013.10.008 doi:10.1016/j.jff.2013.10.008
spellingShingle Probiotics; Heat stress; Gastrointestinal tolerance; Sodium alginate; HPMC; Chitosan
Yonekura, Lina
Sun, Han
Soukoulis, Christos
Fisk, Ian D.
Microencapsulation of Lactobacillus acidophilus NCIMB 701748 in matrices containing soluble fibre by spray drying: technological characterization, storage stability and survival after in vitro digestion
title Microencapsulation of Lactobacillus acidophilus NCIMB 701748 in matrices containing soluble fibre by spray drying: technological characterization, storage stability and survival after in vitro digestion
title_full Microencapsulation of Lactobacillus acidophilus NCIMB 701748 in matrices containing soluble fibre by spray drying: technological characterization, storage stability and survival after in vitro digestion
title_fullStr Microencapsulation of Lactobacillus acidophilus NCIMB 701748 in matrices containing soluble fibre by spray drying: technological characterization, storage stability and survival after in vitro digestion
title_full_unstemmed Microencapsulation of Lactobacillus acidophilus NCIMB 701748 in matrices containing soluble fibre by spray drying: technological characterization, storage stability and survival after in vitro digestion
title_short Microencapsulation of Lactobacillus acidophilus NCIMB 701748 in matrices containing soluble fibre by spray drying: technological characterization, storage stability and survival after in vitro digestion
title_sort microencapsulation of lactobacillus acidophilus ncimb 701748 in matrices containing soluble fibre by spray drying: technological characterization, storage stability and survival after in vitro digestion
topic Probiotics; Heat stress; Gastrointestinal tolerance; Sodium alginate; HPMC; Chitosan
url https://eprints.nottingham.ac.uk/28790/
https://eprints.nottingham.ac.uk/28790/
https://eprints.nottingham.ac.uk/28790/