Lability of copper bound to humic acid

Geochemical speciation models generally include the assumption that all metal bound to humic acid and fulvic acid (HA, FA) is labile. However, in the current study, we determined the presence of a soluble ‘non-labile’ Cu fraction bound to HA extracted from grassland and peat soils. This was quantif...

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Main Authors: Mao, Lingchen, Young, Scott D., Bailey, Liz
Format: Article
Published: Elsevier 2015
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Online Access:https://eprints.nottingham.ac.uk/28778/
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author Mao, Lingchen
Young, Scott D.
Bailey, Liz
author_facet Mao, Lingchen
Young, Scott D.
Bailey, Liz
author_sort Mao, Lingchen
building Nottingham Research Data Repository
collection Online Access
description Geochemical speciation models generally include the assumption that all metal bound to humic acid and fulvic acid (HA, FA) is labile. However, in the current study, we determined the presence of a soluble ‘non-labile’ Cu fraction bound to HA extracted from grassland and peat soils. This was quantified by determining isotopically-exchangeable Cu (E-value) and EDTA-extraction of HA-bound Cu, separated by size-exclusion chromatography (SEC) and assayed by coupled ICP-MS. Evidence of time-dependent Cu fixation by HA was found during the course of an incubation study (160 days); up to 50% of dissolved HA-bound Cu was not isotopically exchangeable. This result was supported by extraction with EDTA where approximately 40% of Cu remained bound to HA despite dissolution in 0.05 M Na2-EDTA. The presence of a substantial non-labile metal fraction held by HA challenges the assumption of wholly reversible equilibrium which is central to current geochemical models of metal binding to humic substances.
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spelling nottingham-287782020-05-04T17:09:21Z https://eprints.nottingham.ac.uk/28778/ Lability of copper bound to humic acid Mao, Lingchen Young, Scott D. Bailey, Liz Geochemical speciation models generally include the assumption that all metal bound to humic acid and fulvic acid (HA, FA) is labile. However, in the current study, we determined the presence of a soluble ‘non-labile’ Cu fraction bound to HA extracted from grassland and peat soils. This was quantified by determining isotopically-exchangeable Cu (E-value) and EDTA-extraction of HA-bound Cu, separated by size-exclusion chromatography (SEC) and assayed by coupled ICP-MS. Evidence of time-dependent Cu fixation by HA was found during the course of an incubation study (160 days); up to 50% of dissolved HA-bound Cu was not isotopically exchangeable. This result was supported by extraction with EDTA where approximately 40% of Cu remained bound to HA despite dissolution in 0.05 M Na2-EDTA. The presence of a substantial non-labile metal fraction held by HA challenges the assumption of wholly reversible equilibrium which is central to current geochemical models of metal binding to humic substances. Elsevier 2015-07-01 Article PeerReviewed Mao, Lingchen, Young, Scott D. and Bailey, Liz (2015) Lability of copper bound to humic acid. Chemosphere, 131 . pp. 201-208. ISSN 0045-6535 Humic acid Non-labile metal WHAM model Isotopic dilution EDTA extraction SEC-ICP-MS http://www.sciencedirect.com/science/article/pii/S0045653515002453 doi:10.1016/j.chemosphere.2015.03.035 doi:10.1016/j.chemosphere.2015.03.035
spellingShingle Humic acid
Non-labile metal
WHAM model
Isotopic dilution
EDTA extraction
SEC-ICP-MS
Mao, Lingchen
Young, Scott D.
Bailey, Liz
Lability of copper bound to humic acid
title Lability of copper bound to humic acid
title_full Lability of copper bound to humic acid
title_fullStr Lability of copper bound to humic acid
title_full_unstemmed Lability of copper bound to humic acid
title_short Lability of copper bound to humic acid
title_sort lability of copper bound to humic acid
topic Humic acid
Non-labile metal
WHAM model
Isotopic dilution
EDTA extraction
SEC-ICP-MS
url https://eprints.nottingham.ac.uk/28778/
https://eprints.nottingham.ac.uk/28778/
https://eprints.nottingham.ac.uk/28778/