'Candidatus phytoplasma palmicola’: a novel taxon associated with a lethal yellowing-type disease (LYD) of coconut ( Cocos nucifera L.) in Mozambique
In this study, the taxonomic position and group classification of the phytoplasma associated with a lethal yellowing-type disease (LYD) of coconut (Cocos nucifera L.) in Mozambique were addressed. Pairwise sequence similarity values based on alignment of near full-length 16SrRNA genes (1530 bp) reve...
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| Format: | Article |
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Society for General Microbiology
2014
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| Online Access: | https://eprints.nottingham.ac.uk/28698/ |
| _version_ | 1848793626883129344 |
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| author | Harrison, Nigel A. Davis, Robert E. Oropeza, Carlo Helmick, Erik E. Narvaez, Maria Eden-Green, Simon Dollet, Michel Dickinson, Matthew Konan Konan, Jean L. |
| author_facet | Harrison, Nigel A. Davis, Robert E. Oropeza, Carlo Helmick, Erik E. Narvaez, Maria Eden-Green, Simon Dollet, Michel Dickinson, Matthew Konan Konan, Jean L. |
| author_sort | Harrison, Nigel A. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | In this study, the taxonomic position and group classification of the phytoplasma associated with a lethal yellowing-type disease (LYD) of coconut (Cocos nucifera L.) in Mozambique were addressed. Pairwise sequence similarity values based on alignment of near full-length 16SrRNA genes (1530 bp) revealed that the Mozambique coconut phytoplasma (LYDM) shared 100% identity with a comparable sequence derived from a phytoplasma strain (LDN), responsible for Awka wilt disease of coconut in Nigeria, and shared 99-99.6% identity with 16S rRNA sequences from strains associated with Cape St. Paul wilt (CSPW) disease of coconut in Ghana and Côte d'Ivoire. Similarity scores further determined the 16S rRNA gene of LYDM phytoplasma to share <97.5% sequence identity with all prior descriptions of ‘Ca. Phytoplasma’ species. Presence of unique regions in the 16S rRNA distinguished LYDM phytoplasma from all currently described ‘Candidatus Phytoplasma’ species, justifying its recognition as reference strain of a novel taxon, ‘Candidatus Phytoplasma palmicola’. Restriction fragment length polymorphism (RFLP) profiles of the F2n/R2 portion (1251 bp) of the 16S rRNA gene and pattern similarity coefficient values delineated coconut LYDM phytoplasma strains from Mozambique as new members of established group 16SrXXII, subgroup A (16SrXXII-A). Similarity coefficients of 0.97 were obtained for comparisons between subgroup 16SrXXII-A strains and CSPW phytoplasmas from Ghana and Côte d'Ivoire. On this basis, CSPW phytoplasma strains were designated as members of a new subgroup, 16SrXXII-B. |
| first_indexed | 2025-11-14T19:03:18Z |
| format | Article |
| id | nottingham-28698 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T19:03:18Z |
| publishDate | 2014 |
| publisher | Society for General Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-286982024-08-15T15:14:49Z https://eprints.nottingham.ac.uk/28698/ 'Candidatus phytoplasma palmicola’: a novel taxon associated with a lethal yellowing-type disease (LYD) of coconut ( Cocos nucifera L.) in Mozambique Harrison, Nigel A. Davis, Robert E. Oropeza, Carlo Helmick, Erik E. Narvaez, Maria Eden-Green, Simon Dollet, Michel Dickinson, Matthew Konan Konan, Jean L. In this study, the taxonomic position and group classification of the phytoplasma associated with a lethal yellowing-type disease (LYD) of coconut (Cocos nucifera L.) in Mozambique were addressed. Pairwise sequence similarity values based on alignment of near full-length 16SrRNA genes (1530 bp) revealed that the Mozambique coconut phytoplasma (LYDM) shared 100% identity with a comparable sequence derived from a phytoplasma strain (LDN), responsible for Awka wilt disease of coconut in Nigeria, and shared 99-99.6% identity with 16S rRNA sequences from strains associated with Cape St. Paul wilt (CSPW) disease of coconut in Ghana and Côte d'Ivoire. Similarity scores further determined the 16S rRNA gene of LYDM phytoplasma to share <97.5% sequence identity with all prior descriptions of ‘Ca. Phytoplasma’ species. Presence of unique regions in the 16S rRNA distinguished LYDM phytoplasma from all currently described ‘Candidatus Phytoplasma’ species, justifying its recognition as reference strain of a novel taxon, ‘Candidatus Phytoplasma palmicola’. Restriction fragment length polymorphism (RFLP) profiles of the F2n/R2 portion (1251 bp) of the 16S rRNA gene and pattern similarity coefficient values delineated coconut LYDM phytoplasma strains from Mozambique as new members of established group 16SrXXII, subgroup A (16SrXXII-A). Similarity coefficients of 0.97 were obtained for comparisons between subgroup 16SrXXII-A strains and CSPW phytoplasmas from Ghana and Côte d'Ivoire. On this basis, CSPW phytoplasma strains were designated as members of a new subgroup, 16SrXXII-B. Society for General Microbiology 2014-02-28 Article PeerReviewed Harrison, Nigel A., Davis, Robert E., Oropeza, Carlo, Helmick, Erik E., Narvaez, Maria, Eden-Green, Simon, Dollet, Michel, Dickinson, Matthew and Konan Konan, Jean L. (2014) 'Candidatus phytoplasma palmicola’: a novel taxon associated with a lethal yellowing-type disease (LYD) of coconut ( Cocos nucifera L.) in Mozambique. International Journal of Systematic and Evolutionary Microbiology, 64 (6). pp. 1890-1899. ISSN 1466-5026 http://ijs.sgmjournals.org/content/64/Pt_6/1890.abstract doi:10.1099/ijs.0.060053-0 doi:10.1099/ijs.0.060053-0 |
| spellingShingle | Harrison, Nigel A. Davis, Robert E. Oropeza, Carlo Helmick, Erik E. Narvaez, Maria Eden-Green, Simon Dollet, Michel Dickinson, Matthew Konan Konan, Jean L. 'Candidatus phytoplasma palmicola’: a novel taxon associated with a lethal yellowing-type disease (LYD) of coconut ( Cocos nucifera L.) in Mozambique |
| title | 'Candidatus phytoplasma palmicola’: a novel taxon associated with a lethal yellowing-type disease (LYD) of coconut ( Cocos nucifera L.) in Mozambique |
| title_full | 'Candidatus phytoplasma palmicola’: a novel taxon associated with a lethal yellowing-type disease (LYD) of coconut ( Cocos nucifera L.) in Mozambique |
| title_fullStr | 'Candidatus phytoplasma palmicola’: a novel taxon associated with a lethal yellowing-type disease (LYD) of coconut ( Cocos nucifera L.) in Mozambique |
| title_full_unstemmed | 'Candidatus phytoplasma palmicola’: a novel taxon associated with a lethal yellowing-type disease (LYD) of coconut ( Cocos nucifera L.) in Mozambique |
| title_short | 'Candidatus phytoplasma palmicola’: a novel taxon associated with a lethal yellowing-type disease (LYD) of coconut ( Cocos nucifera L.) in Mozambique |
| title_sort | 'candidatus phytoplasma palmicola’: a novel taxon associated with a lethal yellowing-type disease (lyd) of coconut ( cocos nucifera l.) in mozambique |
| url | https://eprints.nottingham.ac.uk/28698/ https://eprints.nottingham.ac.uk/28698/ https://eprints.nottingham.ac.uk/28698/ |