Highly sensitive multipoint real-time kinetic detection of Surface Plasmon bioanalytes with custom CMOS cameras
Phase sensitive Surface Plasmon Resonance (SPR) techniques are a popular means of characterizing biomolecular interactions. However, limitations due to the narrow dynamic range and difficulty in adapting the method for multi-point sensing have restricted its range of applications. This paper present...
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| Format: | Article |
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Elsevier
2014
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| Online Access: | https://eprints.nottingham.ac.uk/2867/ |
| _version_ | 1848790895727476736 |
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| author | Wang, Jing Smith, Richard J. Light, Roger Richens, Jo Zhang, Jing O'Shea, Paul See, Chung Somekh, Michael G. |
| author_facet | Wang, Jing Smith, Richard J. Light, Roger Richens, Jo Zhang, Jing O'Shea, Paul See, Chung Somekh, Michael G. |
| author_sort | Wang, Jing |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Phase sensitive Surface Plasmon Resonance (SPR) techniques are a popular means of characterizing biomolecular interactions. However, limitations due to the narrow dynamic range and difficulty in adapting the method for multi-point sensing have restricted its range of applications. This paper presents a compact phase sensitive SPR technology using a custom CMOS camera. The system is exceptionally versatile enabling one to trade dynamic range for sensitivity without altering the optical system. We present results showing sensitivity over the array of better than 10−6 Refractive Index Units (RIU) over a refractive index range of 2×10−2 RIU, with peak sensitivity of 3×10−7 RIU at the center of this range. We also explain how simply altering the settings of polarization components can give sensitivity on the order of 10−8 RIU albeit at the cost of lower dynamic range. The consistent response of the custom CMOS camera in the system also allowed us to demonstrate precise quantitative detection of two Fibrinogen antibody–protein binding sites. Moreover, we use the system to determine reaction kinetics and argue how the multipoint detection gives useful insight into the molecular binding mechanisms. |
| first_indexed | 2025-11-14T18:19:53Z |
| format | Article |
| id | nottingham-2867 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:19:53Z |
| publishDate | 2014 |
| publisher | Elsevier |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-28672020-05-04T16:52:26Z https://eprints.nottingham.ac.uk/2867/ Highly sensitive multipoint real-time kinetic detection of Surface Plasmon bioanalytes with custom CMOS cameras Wang, Jing Smith, Richard J. Light, Roger Richens, Jo Zhang, Jing O'Shea, Paul See, Chung Somekh, Michael G. Phase sensitive Surface Plasmon Resonance (SPR) techniques are a popular means of characterizing biomolecular interactions. However, limitations due to the narrow dynamic range and difficulty in adapting the method for multi-point sensing have restricted its range of applications. This paper presents a compact phase sensitive SPR technology using a custom CMOS camera. The system is exceptionally versatile enabling one to trade dynamic range for sensitivity without altering the optical system. We present results showing sensitivity over the array of better than 10−6 Refractive Index Units (RIU) over a refractive index range of 2×10−2 RIU, with peak sensitivity of 3×10−7 RIU at the center of this range. We also explain how simply altering the settings of polarization components can give sensitivity on the order of 10−8 RIU albeit at the cost of lower dynamic range. The consistent response of the custom CMOS camera in the system also allowed us to demonstrate precise quantitative detection of two Fibrinogen antibody–protein binding sites. Moreover, we use the system to determine reaction kinetics and argue how the multipoint detection gives useful insight into the molecular binding mechanisms. Elsevier 2014-08-15 Article PeerReviewed Wang, Jing, Smith, Richard J., Light, Roger, Richens, Jo, Zhang, Jing, O'Shea, Paul, See, Chung and Somekh, Michael G. (2014) Highly sensitive multipoint real-time kinetic detection of Surface Plasmon bioanalytes with custom CMOS cameras. Biosensors and Bioelectronics, 58 . pp. 157-164. ISSN 0956-5663 http://www.sciencedirect.com/science/article/pii/S0956566314001304 doi:10.1016/j.bios.2014.02.042 doi:10.1016/j.bios.2014.02.042 |
| spellingShingle | Wang, Jing Smith, Richard J. Light, Roger Richens, Jo Zhang, Jing O'Shea, Paul See, Chung Somekh, Michael G. Highly sensitive multipoint real-time kinetic detection of Surface Plasmon bioanalytes with custom CMOS cameras |
| title | Highly sensitive multipoint real-time kinetic detection of Surface Plasmon bioanalytes with custom CMOS cameras |
| title_full | Highly sensitive multipoint real-time kinetic detection of Surface Plasmon bioanalytes with custom CMOS cameras |
| title_fullStr | Highly sensitive multipoint real-time kinetic detection of Surface Plasmon bioanalytes with custom CMOS cameras |
| title_full_unstemmed | Highly sensitive multipoint real-time kinetic detection of Surface Plasmon bioanalytes with custom CMOS cameras |
| title_short | Highly sensitive multipoint real-time kinetic detection of Surface Plasmon bioanalytes with custom CMOS cameras |
| title_sort | highly sensitive multipoint real-time kinetic detection of surface plasmon bioanalytes with custom cmos cameras |
| url | https://eprints.nottingham.ac.uk/2867/ https://eprints.nottingham.ac.uk/2867/ https://eprints.nottingham.ac.uk/2867/ |