Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI
Ex vivo rodent lung models are explored for physiological measurements of respiratory function with hyperpolarized (hp) 129Xe MRI. It is shown that excised lung models allow for simplification of the technical challenges involved and provide valuable physiological insights that are not feasible usin...
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| Format: | Article |
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Public Library of Science
2013
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| Online Access: | https://eprints.nottingham.ac.uk/2848/ |
| _version_ | 1848790891749179392 |
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| author | Lilburn, David M.L. Hughes-Riley, Theodore Six, Joseph S. Stupic, Karl F. Shaw, Dominick E. Pavlovskaya, Galina E. Meersmann, Thomas |
| author_facet | Lilburn, David M.L. Hughes-Riley, Theodore Six, Joseph S. Stupic, Karl F. Shaw, Dominick E. Pavlovskaya, Galina E. Meersmann, Thomas |
| author_sort | Lilburn, David M.L. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Ex vivo rodent lung models are explored for physiological measurements of respiratory function with hyperpolarized (hp) 129Xe MRI. It is shown that excised lung models allow for simplification of the technical challenges involved and provide valuable physiological insights that are not feasible using in vivo MRI protocols. A custom designed breathing apparatus enables MR images of gas distribution on increasing ventilation volumes of actively inhaled hp 129Xe. Straightforward hp 129Xe MRI protocols provide residual lung volume (RV) data and permit for spatially resolved tracking of small hp 129Xe probe volumes during the inhalation cycle. Hp 129Xe MRI of lung function in the excised organ demonstrates the persistence of post mortem airway responsiveness to intravenous methacholine challenges. The presented methodology enables physiology of lung function in health and disease without additional regulatory approval requirements and reduces the technical and logistical challenges with hp gas MRI experiments. The post mortem lung functional data can augment histological measurements and should be of interest for drug development studies. |
| first_indexed | 2025-11-14T18:19:49Z |
| format | Article |
| id | nottingham-2848 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:19:49Z |
| publishDate | 2013 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-28482020-05-04T16:38:17Z https://eprints.nottingham.ac.uk/2848/ Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI Lilburn, David M.L. Hughes-Riley, Theodore Six, Joseph S. Stupic, Karl F. Shaw, Dominick E. Pavlovskaya, Galina E. Meersmann, Thomas Ex vivo rodent lung models are explored for physiological measurements of respiratory function with hyperpolarized (hp) 129Xe MRI. It is shown that excised lung models allow for simplification of the technical challenges involved and provide valuable physiological insights that are not feasible using in vivo MRI protocols. A custom designed breathing apparatus enables MR images of gas distribution on increasing ventilation volumes of actively inhaled hp 129Xe. Straightforward hp 129Xe MRI protocols provide residual lung volume (RV) data and permit for spatially resolved tracking of small hp 129Xe probe volumes during the inhalation cycle. Hp 129Xe MRI of lung function in the excised organ demonstrates the persistence of post mortem airway responsiveness to intravenous methacholine challenges. The presented methodology enables physiology of lung function in health and disease without additional regulatory approval requirements and reduces the technical and logistical challenges with hp gas MRI experiments. The post mortem lung functional data can augment histological measurements and should be of interest for drug development studies. Public Library of Science 2013-08-30 Article PeerReviewed Lilburn, David M.L., Hughes-Riley, Theodore, Six, Joseph S., Stupic, Karl F., Shaw, Dominick E., Pavlovskaya, Galina E. and Meersmann, Thomas (2013) Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI. PLoS ONE, 8 (8). e73468/1-e73468/13. ISSN 1932-6203 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0073468 doi:10.1371/journal.pone.0073468 doi:10.1371/journal.pone.0073468 |
| spellingShingle | Lilburn, David M.L. Hughes-Riley, Theodore Six, Joseph S. Stupic, Karl F. Shaw, Dominick E. Pavlovskaya, Galina E. Meersmann, Thomas Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI |
| title | Validating excised rodent lungs for functional
hyperpolarized xenon-129 MRI |
| title_full | Validating excised rodent lungs for functional
hyperpolarized xenon-129 MRI |
| title_fullStr | Validating excised rodent lungs for functional
hyperpolarized xenon-129 MRI |
| title_full_unstemmed | Validating excised rodent lungs for functional
hyperpolarized xenon-129 MRI |
| title_short | Validating excised rodent lungs for functional
hyperpolarized xenon-129 MRI |
| title_sort | validating excised rodent lungs for functional
hyperpolarized xenon-129 mri |
| url | https://eprints.nottingham.ac.uk/2848/ https://eprints.nottingham.ac.uk/2848/ https://eprints.nottingham.ac.uk/2848/ |