Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain
Clostridium difficile infection is the main cause of healthcare-acquired diarrhea in the developed world. In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To develop effective medical countermeasures, it is impor...
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| Format: | Article |
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Oxford University Press
2013
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| Online Access: | https://eprints.nottingham.ac.uk/2815/ |
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| author | Kuehne, Sarah A. Collery, Mark M. Kelly, Michelle L. Cartman, Stephen T. Cockayne, Alan Minton, Nigel P. |
| author_facet | Kuehne, Sarah A. Collery, Mark M. Kelly, Michelle L. Cartman, Stephen T. Cockayne, Alan Minton, Nigel P. |
| author_sort | Kuehne, Sarah A. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Clostridium difficile infection is the main cause of healthcare-acquired diarrhea in the developed world. In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To develop effective medical countermeasures, it is important to understand the importance of each toxin. Accordingly, we created all possible combinations of isogenic toxin mutants of R20291 and assessed their virulence. We demonstrated that either toxin A or toxin B alone can cause fulminant disease in the hamster infection model and present tantalizing data that C. difficile toxin may also contribute to virulence. |
| first_indexed | 2025-11-14T18:19:41Z |
| format | Article |
| id | nottingham-2815 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:19:41Z |
| publishDate | 2013 |
| publisher | Oxford University Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-28152020-05-04T16:38:30Z https://eprints.nottingham.ac.uk/2815/ Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain Kuehne, Sarah A. Collery, Mark M. Kelly, Michelle L. Cartman, Stephen T. Cockayne, Alan Minton, Nigel P. Clostridium difficile infection is the main cause of healthcare-acquired diarrhea in the developed world. In addition to the main virulence factors toxin A and B, epidemic, PCR Ribotype 027 strains, such as R20291, produce a third toxin, CDT. To develop effective medical countermeasures, it is important to understand the importance of each toxin. Accordingly, we created all possible combinations of isogenic toxin mutants of R20291 and assessed their virulence. We demonstrated that either toxin A or toxin B alone can cause fulminant disease in the hamster infection model and present tantalizing data that C. difficile toxin may also contribute to virulence. Oxford University Press 2013-08-09 Article PeerReviewed Kuehne, Sarah A., Collery, Mark M., Kelly, Michelle L., Cartman, Stephen T., Cockayne, Alan and Minton, Nigel P. (2013) Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain. Journal of Infectious Diseases, 209 (1). pp. 83-86. ISSN 1537-6613 Clostridium difficile infection; TcdA; TcdB; pathogenesis; CDT. http://jid.oxfordjournals.org/content/209/1/83 doi:10.1093/infdis/jit426 doi:10.1093/infdis/jit426 |
| spellingShingle | Clostridium difficile infection; TcdA; TcdB; pathogenesis; CDT. Kuehne, Sarah A. Collery, Mark M. Kelly, Michelle L. Cartman, Stephen T. Cockayne, Alan Minton, Nigel P. Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain |
| title | Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain |
| title_full | Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain |
| title_fullStr | Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain |
| title_full_unstemmed | Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain |
| title_short | Importance of Toxin A, Toxin B, and CDT in virulence of an epidemic Clostridium difficile strain |
| title_sort | importance of toxin a, toxin b, and cdt in virulence of an epidemic clostridium difficile strain |
| topic | Clostridium difficile infection; TcdA; TcdB; pathogenesis; CDT. |
| url | https://eprints.nottingham.ac.uk/2815/ https://eprints.nottingham.ac.uk/2815/ https://eprints.nottingham.ac.uk/2815/ |