Caspase-cleaved cytokeratin-18 and tumour regression in gastro-oesophageal adenocarcinomas treated with neoadjuvant chemotherapy
AIM: To examine cytokeratin-18 (CK-18) and caspase-cleaved CK-18 expression in tumours and correlate with clinicopathological outcomes including tumour regression grade (TRG) response. METHODS: Formalin-fixed human gastro-oesophageal cancers were constructed into tissue microarrays. The first set...
| Main Authors: | , , , , , , |
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| Format: | Article |
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Baishideng Publishing Group
2012
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| Online Access: | https://eprints.nottingham.ac.uk/2797/ |
| _version_ | 1848790878146002944 |
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| author | Fareed, Khaleel R. Soomro, Irshad N. Hameed, Khalid Arora, Arvind Lobo, Dileep N. Parsons, Simon L. Madhusudan, Srinivasan |
| author_facet | Fareed, Khaleel R. Soomro, Irshad N. Hameed, Khalid Arora, Arvind Lobo, Dileep N. Parsons, Simon L. Madhusudan, Srinivasan |
| author_sort | Fareed, Khaleel R. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | AIM: To examine cytokeratin-18 (CK-18) and caspase-cleaved CK-18 expression in tumours and correlate with clinicopathological outcomes including tumour regression grade (TRG) response.
METHODS: Formalin-fixed human gastro-oesophageal cancers were constructed into tissue microarrays. The first set consisted of 122 gastric/gastro-oesophageal cancer cases not exposed to neoadjuvant chemotherapy and the second set consisted of 97 gastric/gastro-oesophageal cancer cases exposed to pre-operative platinum-based chemotherapy. Expression of CK-18 and caspase-cleaved CK-18 was investigated using immunohistochemistry.
RESULTS: CK18 was commonly expressed in gastro-oesophageal tumours (92.6%). Fifty-six point seven percent of tumours previously exposed to neoadjuvant chemotherapy were positive for caspase-cleaved CK-18 expression compared to only 24.6% of tumours not previously exposed to neoadjuvant chemotherapy (P = 0.009). In patients who received neoadjuvant chemotherapy, caspase-cleaved cytokeratin-18 expression correlated with favourable TRG response (TRG 1, 2 or 3, P = 0.043).
CONCLUSION: This is the largest study to date of CK-18 and caspase-cleaved CK-18 expression in gastro-oesophageal tumours. We provide the first evidence that caspase-cleaved CK-18 predicts tumour regression with neoadjuvant chemotherapy. |
| first_indexed | 2025-11-14T18:19:36Z |
| format | Article |
| id | nottingham-2797 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:19:36Z |
| publishDate | 2012 |
| publisher | Baishideng Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-27972020-05-04T16:32:53Z https://eprints.nottingham.ac.uk/2797/ Caspase-cleaved cytokeratin-18 and tumour regression in gastro-oesophageal adenocarcinomas treated with neoadjuvant chemotherapy Fareed, Khaleel R. Soomro, Irshad N. Hameed, Khalid Arora, Arvind Lobo, Dileep N. Parsons, Simon L. Madhusudan, Srinivasan AIM: To examine cytokeratin-18 (CK-18) and caspase-cleaved CK-18 expression in tumours and correlate with clinicopathological outcomes including tumour regression grade (TRG) response. METHODS: Formalin-fixed human gastro-oesophageal cancers were constructed into tissue microarrays. The first set consisted of 122 gastric/gastro-oesophageal cancer cases not exposed to neoadjuvant chemotherapy and the second set consisted of 97 gastric/gastro-oesophageal cancer cases exposed to pre-operative platinum-based chemotherapy. Expression of CK-18 and caspase-cleaved CK-18 was investigated using immunohistochemistry. RESULTS: CK18 was commonly expressed in gastro-oesophageal tumours (92.6%). Fifty-six point seven percent of tumours previously exposed to neoadjuvant chemotherapy were positive for caspase-cleaved CK-18 expression compared to only 24.6% of tumours not previously exposed to neoadjuvant chemotherapy (P = 0.009). In patients who received neoadjuvant chemotherapy, caspase-cleaved cytokeratin-18 expression correlated with favourable TRG response (TRG 1, 2 or 3, P = 0.043). CONCLUSION: This is the largest study to date of CK-18 and caspase-cleaved CK-18 expression in gastro-oesophageal tumours. We provide the first evidence that caspase-cleaved CK-18 predicts tumour regression with neoadjuvant chemotherapy. Baishideng Publishing Group 2012-04-28 Article PeerReviewed Fareed, Khaleel R., Soomro, Irshad N., Hameed, Khalid, Arora, Arvind, Lobo, Dileep N., Parsons, Simon L. and Madhusudan, Srinivasan (2012) Caspase-cleaved cytokeratin-18 and tumour regression in gastro-oesophageal adenocarcinomas treated with neoadjuvant chemotherapy. World Journal of Gastroenterology, 18 (16). pp. 1915-1920. ISSN 1007-9327 http://www.wjgnet.com/1007-9327/abstract/v18/i16/1915.htm doi:10.3748/wjg.v18.i16.1915 doi:10.3748/wjg.v18.i16.1915 |
| spellingShingle | Fareed, Khaleel R. Soomro, Irshad N. Hameed, Khalid Arora, Arvind Lobo, Dileep N. Parsons, Simon L. Madhusudan, Srinivasan Caspase-cleaved cytokeratin-18 and tumour regression in gastro-oesophageal adenocarcinomas treated with neoadjuvant chemotherapy |
| title | Caspase-cleaved cytokeratin-18 and tumour regression in gastro-oesophageal adenocarcinomas treated with neoadjuvant chemotherapy |
| title_full | Caspase-cleaved cytokeratin-18 and tumour regression in gastro-oesophageal adenocarcinomas treated with neoadjuvant chemotherapy |
| title_fullStr | Caspase-cleaved cytokeratin-18 and tumour regression in gastro-oesophageal adenocarcinomas treated with neoadjuvant chemotherapy |
| title_full_unstemmed | Caspase-cleaved cytokeratin-18 and tumour regression in gastro-oesophageal adenocarcinomas treated with neoadjuvant chemotherapy |
| title_short | Caspase-cleaved cytokeratin-18 and tumour regression in gastro-oesophageal adenocarcinomas treated with neoadjuvant chemotherapy |
| title_sort | caspase-cleaved cytokeratin-18 and tumour regression in gastro-oesophageal adenocarcinomas treated with neoadjuvant chemotherapy |
| url | https://eprints.nottingham.ac.uk/2797/ https://eprints.nottingham.ac.uk/2797/ https://eprints.nottingham.ac.uk/2797/ |