DNA damage induces nucleoid compaction via the Mre11-Rad50 complex in the archaeon Haloferax volcanii
In prokaryotes the genome is organized in a dynamic structure called the nucleoid, which is embedded in the cytoplasm. We show here that in the archaeon Haloferax volcanii, compaction and reorganization of the nucleoid is induced by stresses that damage the genome or interfere with its replicat...
| Main Authors: | , , |
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| Format: | Article |
| Published: |
Wiley
2013
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| Online Access: | https://eprints.nottingham.ac.uk/2792/ |
| _version_ | 1848790876762931200 |
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| author | Delmas, Stéphane Duggin, Iain J. Allers, Thorsten |
| author_facet | Delmas, Stéphane Duggin, Iain J. Allers, Thorsten |
| author_sort | Delmas, Stéphane |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | In prokaryotes the genome is organized in a dynamic
structure called the nucleoid, which is embedded in
the cytoplasm. We show here that in the archaeon
Haloferax volcanii, compaction and reorganization of
the nucleoid is induced by stresses that damage the
genome or interfere with its replication. The fraction
of cells exhibiting nucleoid compaction was proportional
to the dose of the DNA damaging agent, and
results obtained in cells defective for nucleotide excision
repair suggest that breakage of DNA strands
triggers reorganization of the nucleoid. We observed
that compaction depends on the Mre11-Rad50
complex, suggesting a link to DNA double-strand
break repair. However, compaction was observed in a
radA mutant, indicating that the role of Mre11-Rad50
in nucleoid reorganisation is independent of homologous
recombination. We therefore propose that
nucleoid compaction is part of a DNA damage
response that accelerates cell recovery by helping
DNA repair proteins to locate their targets, and facilitating
the search for intact DNA sequences during
homologous recombination. |
| first_indexed | 2025-11-14T18:19:35Z |
| format | Article |
| id | nottingham-2792 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:19:35Z |
| publishDate | 2013 |
| publisher | Wiley |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-27922020-05-04T20:19:40Z https://eprints.nottingham.ac.uk/2792/ DNA damage induces nucleoid compaction via the Mre11-Rad50 complex in the archaeon Haloferax volcanii Delmas, Stéphane Duggin, Iain J. Allers, Thorsten In prokaryotes the genome is organized in a dynamic structure called the nucleoid, which is embedded in the cytoplasm. We show here that in the archaeon Haloferax volcanii, compaction and reorganization of the nucleoid is induced by stresses that damage the genome or interfere with its replication. The fraction of cells exhibiting nucleoid compaction was proportional to the dose of the DNA damaging agent, and results obtained in cells defective for nucleotide excision repair suggest that breakage of DNA strands triggers reorganization of the nucleoid. We observed that compaction depends on the Mre11-Rad50 complex, suggesting a link to DNA double-strand break repair. However, compaction was observed in a radA mutant, indicating that the role of Mre11-Rad50 in nucleoid reorganisation is independent of homologous recombination. We therefore propose that nucleoid compaction is part of a DNA damage response that accelerates cell recovery by helping DNA repair proteins to locate their targets, and facilitating the search for intact DNA sequences during homologous recombination. Wiley 2013-01 Article PeerReviewed Delmas, Stéphane, Duggin, Iain J. and Allers, Thorsten (2013) DNA damage induces nucleoid compaction via the Mre11-Rad50 complex in the archaeon Haloferax volcanii. Molecular Microbiology, 87 (1). pp. 168-179. ISSN 0950-382X http://onlinelibrary.wiley.com/doi/10.1111/mmi.12091/abstract doi:10.1111/mmi.12091 doi:10.1111/mmi.12091 |
| spellingShingle | Delmas, Stéphane Duggin, Iain J. Allers, Thorsten DNA damage induces nucleoid compaction via the Mre11-Rad50 complex in the archaeon Haloferax volcanii |
| title | DNA damage induces nucleoid compaction via the
Mre11-Rad50 complex in the archaeon Haloferax volcanii |
| title_full | DNA damage induces nucleoid compaction via the
Mre11-Rad50 complex in the archaeon Haloferax volcanii |
| title_fullStr | DNA damage induces nucleoid compaction via the
Mre11-Rad50 complex in the archaeon Haloferax volcanii |
| title_full_unstemmed | DNA damage induces nucleoid compaction via the
Mre11-Rad50 complex in the archaeon Haloferax volcanii |
| title_short | DNA damage induces nucleoid compaction via the
Mre11-Rad50 complex in the archaeon Haloferax volcanii |
| title_sort | dna damage induces nucleoid compaction via the
mre11-rad50 complex in the archaeon haloferax volcanii |
| url | https://eprints.nottingham.ac.uk/2792/ https://eprints.nottingham.ac.uk/2792/ https://eprints.nottingham.ac.uk/2792/ |