Hsmar1 transposition is sensitive to the topology of the transposon donor and the target
Hsmar1 is a member of the Tc1-mariner superfamily of DNA transposons. These elements mobilize within the genome of their host by a cut-and-paste mechanism. We have exploited the in vitro reaction provided by Hsmar1 to investigate the effect of DNA supercoiling on transposon integration. We found tha...
| Main Authors: | , |
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| Format: | Article |
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Public Library of Science
2013
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| Online Access: | https://eprints.nottingham.ac.uk/2771/ |
| _version_ | 1848790871424630784 |
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| author | Claeys Bouuaert, Corentin Chalmers, Ronald |
| author_facet | Claeys Bouuaert, Corentin Chalmers, Ronald |
| author_sort | Claeys Bouuaert, Corentin |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Hsmar1 is a member of the Tc1-mariner superfamily of DNA transposons. These elements mobilize within the genome of their host by a cut-and-paste mechanism. We have exploited the in vitro reaction provided by Hsmar1 to investigate the effect of DNA supercoiling on transposon integration. We found that the topology of both the transposon and the target affect integration. Relaxed transposons have an integration defect that can be partially restored in the presence of elevated levels of negatively supercoiled target DNA. Negatively supercoiled DNA is a better target than nicked or positively supercoiled DNA, suggesting that underwinding of the DNA helix promotes target interactions. Like other Tc1-mariner elements, Hsmar1 integrates into 5′-TA dinucleotides. The direct vicinity of the target TA provides little sequence specificity for target interactions. However, transposition within a plasmid substrate was not random and some TA dinucleotides were targeted preferentially. The distribution of intramolecular target sites was not affected by DNA topology. |
| first_indexed | 2025-11-14T18:19:30Z |
| format | Article |
| id | nottingham-2771 |
| institution | University of Nottingham Malaysia Campus |
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| last_indexed | 2025-11-14T18:19:30Z |
| publishDate | 2013 |
| publisher | Public Library of Science |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-27712020-05-04T16:35:28Z https://eprints.nottingham.ac.uk/2771/ Hsmar1 transposition is sensitive to the topology of the transposon donor and the target Claeys Bouuaert, Corentin Chalmers, Ronald Hsmar1 is a member of the Tc1-mariner superfamily of DNA transposons. These elements mobilize within the genome of their host by a cut-and-paste mechanism. We have exploited the in vitro reaction provided by Hsmar1 to investigate the effect of DNA supercoiling on transposon integration. We found that the topology of both the transposon and the target affect integration. Relaxed transposons have an integration defect that can be partially restored in the presence of elevated levels of negatively supercoiled target DNA. Negatively supercoiled DNA is a better target than nicked or positively supercoiled DNA, suggesting that underwinding of the DNA helix promotes target interactions. Like other Tc1-mariner elements, Hsmar1 integrates into 5′-TA dinucleotides. The direct vicinity of the target TA provides little sequence specificity for target interactions. However, transposition within a plasmid substrate was not random and some TA dinucleotides were targeted preferentially. The distribution of intramolecular target sites was not affected by DNA topology. Public Library of Science 2013-01-14 Article PeerReviewed Claeys Bouuaert, Corentin and Chalmers, Ronald (2013) Hsmar1 transposition is sensitive to the topology of the transposon donor and the target. PLoS ONE, 8 (1). 0053690/1-0053690/9. ISSN 1932-6203 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0053690 doi:10.1371/journal.pone.0053690 doi:10.1371/journal.pone.0053690 |
| spellingShingle | Claeys Bouuaert, Corentin Chalmers, Ronald Hsmar1 transposition is sensitive to the topology of the transposon donor and the target |
| title | Hsmar1 transposition is sensitive to the topology of the
transposon donor and the target |
| title_full | Hsmar1 transposition is sensitive to the topology of the
transposon donor and the target |
| title_fullStr | Hsmar1 transposition is sensitive to the topology of the
transposon donor and the target |
| title_full_unstemmed | Hsmar1 transposition is sensitive to the topology of the
transposon donor and the target |
| title_short | Hsmar1 transposition is sensitive to the topology of the
transposon donor and the target |
| title_sort | hsmar1 transposition is sensitive to the topology of the
transposon donor and the target |
| url | https://eprints.nottingham.ac.uk/2771/ https://eprints.nottingham.ac.uk/2771/ https://eprints.nottingham.ac.uk/2771/ |