Study of NAP adsorption and assembly on the surface of HOPG

NAP is an octapeptide that has demonstrated a neuroprotective/therapeutic efficacy at very low concentrations in preclinical studies and in a number of clinical trials. Yet little is known about its structural organization at low concentrations. Here, we have employed atomic force microscopy to inve...

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Main Authors: Korolkov, Vladimir V., Allen, Stephanie, Roberts, Clive J., Gozes, Illana, Tendler, Saul J.B.
Format: Article
Published: Elsevier 2014
Subjects:
Online Access:https://eprints.nottingham.ac.uk/27680/
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author Korolkov, Vladimir V.
Allen, Stephanie
Roberts, Clive J.
Gozes, Illana
Tendler, Saul J.B.
author_facet Korolkov, Vladimir V.
Allen, Stephanie
Roberts, Clive J.
Gozes, Illana
Tendler, Saul J.B.
author_sort Korolkov, Vladimir V.
building Nottingham Research Data Repository
collection Online Access
description NAP is an octapeptide that has demonstrated a neuroprotective/therapeutic efficacy at very low concentrations in preclinical studies and in a number of clinical trials. Yet little is known about its structural organization at low concentrations. Here, we have employed atomic force microscopy to investigate NAP peptide assembly on graphite in aqueous media at nanomolar concentration. High spatial resolution scans of NAP assemblies reveal their fine structure with clearly resolved single NAP units. This observation leads us to conclude that NAP molecules do not form complex self-assembled structures at nanomolar concentration when adsorbed on graphite surface.
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spelling nottingham-276802020-05-04T16:53:13Z https://eprints.nottingham.ac.uk/27680/ Study of NAP adsorption and assembly on the surface of HOPG Korolkov, Vladimir V. Allen, Stephanie Roberts, Clive J. Gozes, Illana Tendler, Saul J.B. NAP is an octapeptide that has demonstrated a neuroprotective/therapeutic efficacy at very low concentrations in preclinical studies and in a number of clinical trials. Yet little is known about its structural organization at low concentrations. Here, we have employed atomic force microscopy to investigate NAP peptide assembly on graphite in aqueous media at nanomolar concentration. High spatial resolution scans of NAP assemblies reveal their fine structure with clearly resolved single NAP units. This observation leads us to conclude that NAP molecules do not form complex self-assembled structures at nanomolar concentration when adsorbed on graphite surface. Elsevier 2014-09-30 Article PeerReviewed Korolkov, Vladimir V., Allen, Stephanie, Roberts, Clive J., Gozes, Illana and Tendler, Saul J.B. (2014) Study of NAP adsorption and assembly on the surface of HOPG. Peptides, 62 . pp. 55-58. ISSN 0196-9781 NAP; Atomic Force Microscopy; davunetide; adsorption; peptides http://www.sciencedirect.com/science/article/pii/S0196978114002861 doi:10.1016/j.peptides.2014.09.017 doi:10.1016/j.peptides.2014.09.017
spellingShingle NAP; Atomic Force Microscopy; davunetide; adsorption; peptides
Korolkov, Vladimir V.
Allen, Stephanie
Roberts, Clive J.
Gozes, Illana
Tendler, Saul J.B.
Study of NAP adsorption and assembly on the surface of HOPG
title Study of NAP adsorption and assembly on the surface of HOPG
title_full Study of NAP adsorption and assembly on the surface of HOPG
title_fullStr Study of NAP adsorption and assembly on the surface of HOPG
title_full_unstemmed Study of NAP adsorption and assembly on the surface of HOPG
title_short Study of NAP adsorption and assembly on the surface of HOPG
title_sort study of nap adsorption and assembly on the surface of hopg
topic NAP; Atomic Force Microscopy; davunetide; adsorption; peptides
url https://eprints.nottingham.ac.uk/27680/
https://eprints.nottingham.ac.uk/27680/
https://eprints.nottingham.ac.uk/27680/