Meristematic cell proliferation and ribosome biogenesis are decoupled in diamagnetically levitated Arabidopsis seedlings
Background: Cell growth and cell proliferation are intimately linked in the presence of Earth’s gravity, but are decoupled under the microgravity conditions present in orbiting spacecraft. New technologies to simulate microgravity conditions for long-duration experiments, with stable environmental c...
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| Format: | Article |
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BioMed Central
2013
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| Online Access: | https://eprints.nottingham.ac.uk/2757/ |
| _version_ | 1848790868476035072 |
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| author | Manzano, Ana Isabel Larkin, Oliver J. Dijkstra, Camelia E. Anthony, Paul Davey, Michael R. Eaves, Laurence Hill, Richard J.A. Herranz, Raul Medina, F. Javier |
| author_facet | Manzano, Ana Isabel Larkin, Oliver J. Dijkstra, Camelia E. Anthony, Paul Davey, Michael R. Eaves, Laurence Hill, Richard J.A. Herranz, Raul Medina, F. Javier |
| author_sort | Manzano, Ana Isabel |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Background: Cell growth and cell proliferation are intimately linked in the presence of Earth’s gravity, but are decoupled under the microgravity conditions present in orbiting spacecraft. New technologies to simulate microgravity conditions for long-duration experiments, with stable environmental conditions, in Earth-based laboratories are required to further our understanding of the effect of extraterrestrial conditions on the growth, development and health of living matter.
Results: We studied the response of transgenic seedlings of Arabidopsis thaliana, containing either the CycB1-GUS proliferation marker or the DR5-GUS auxin-mediated growth marker, to diamagnetic levitation in the bore of a superconducting solenoid magnet. As a control, a second set of seedlings were exposed to a strong magnetic field, but not to levitation forces. A third set was exposed to a strong field and simulated hypergravity (2 g). Cell proliferation and cell growth cytological parameters were measured for each set of seedlings. Nucleolin immunodetection was used as a marker of cell growth. Collectively, the data indicate that these two fundamental cellular processes are decoupled in root meristems, as in microgravity: cell proliferation was enhanced whereas cell growth markers were depleted. These results also demonstrated delocalisation of auxin signalling in the root tip despite the fact that levitation of the seedling as a whole does not prevent the sedimentation of statoliths in the root cells.
Conclusions:In our model system, we found that diamagnetic levitation led to changes that are very similar to those caused by real- [e.g. on board the International Space Station (ISS)] or mechanically-simulated microgravity [e.g. using a Random Positioning Machine (RPM)]. These changes decoupled meristematic cell proliferation from ribosome biogenesis, and altered auxin polar transport. |
| first_indexed | 2025-11-14T18:19:27Z |
| format | Article |
| id | nottingham-2757 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:19:27Z |
| publishDate | 2013 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-27572020-05-04T16:38:58Z https://eprints.nottingham.ac.uk/2757/ Meristematic cell proliferation and ribosome biogenesis are decoupled in diamagnetically levitated Arabidopsis seedlings Manzano, Ana Isabel Larkin, Oliver J. Dijkstra, Camelia E. Anthony, Paul Davey, Michael R. Eaves, Laurence Hill, Richard J.A. Herranz, Raul Medina, F. Javier Background: Cell growth and cell proliferation are intimately linked in the presence of Earth’s gravity, but are decoupled under the microgravity conditions present in orbiting spacecraft. New technologies to simulate microgravity conditions for long-duration experiments, with stable environmental conditions, in Earth-based laboratories are required to further our understanding of the effect of extraterrestrial conditions on the growth, development and health of living matter. Results: We studied the response of transgenic seedlings of Arabidopsis thaliana, containing either the CycB1-GUS proliferation marker or the DR5-GUS auxin-mediated growth marker, to diamagnetic levitation in the bore of a superconducting solenoid magnet. As a control, a second set of seedlings were exposed to a strong magnetic field, but not to levitation forces. A third set was exposed to a strong field and simulated hypergravity (2 g). Cell proliferation and cell growth cytological parameters were measured for each set of seedlings. Nucleolin immunodetection was used as a marker of cell growth. Collectively, the data indicate that these two fundamental cellular processes are decoupled in root meristems, as in microgravity: cell proliferation was enhanced whereas cell growth markers were depleted. These results also demonstrated delocalisation of auxin signalling in the root tip despite the fact that levitation of the seedling as a whole does not prevent the sedimentation of statoliths in the root cells. Conclusions:In our model system, we found that diamagnetic levitation led to changes that are very similar to those caused by real- [e.g. on board the International Space Station (ISS)] or mechanically-simulated microgravity [e.g. using a Random Positioning Machine (RPM)]. These changes decoupled meristematic cell proliferation from ribosome biogenesis, and altered auxin polar transport. BioMed Central 2013-09-05 Article PeerReviewed Manzano, Ana Isabel, Larkin, Oliver J., Dijkstra, Camelia E., Anthony, Paul, Davey, Michael R., Eaves, Laurence, Hill, Richard J.A., Herranz, Raul and Medina, F. Javier (2013) Meristematic cell proliferation and ribosome biogenesis are decoupled in diamagnetically levitated Arabidopsis seedlings. BMC Plant Biology, 13 . 124/1-124/15. ISSN 1471-2229 http://www.biomedcentral.com/1471-2229/13/124 doi:10.1186/1471-2229-13-124 doi:10.1186/1471-2229-13-124 |
| spellingShingle | Manzano, Ana Isabel Larkin, Oliver J. Dijkstra, Camelia E. Anthony, Paul Davey, Michael R. Eaves, Laurence Hill, Richard J.A. Herranz, Raul Medina, F. Javier Meristematic cell proliferation and ribosome biogenesis are decoupled in diamagnetically levitated Arabidopsis seedlings |
| title | Meristematic cell proliferation and ribosome biogenesis are decoupled in diamagnetically levitated Arabidopsis seedlings |
| title_full | Meristematic cell proliferation and ribosome biogenesis are decoupled in diamagnetically levitated Arabidopsis seedlings |
| title_fullStr | Meristematic cell proliferation and ribosome biogenesis are decoupled in diamagnetically levitated Arabidopsis seedlings |
| title_full_unstemmed | Meristematic cell proliferation and ribosome biogenesis are decoupled in diamagnetically levitated Arabidopsis seedlings |
| title_short | Meristematic cell proliferation and ribosome biogenesis are decoupled in diamagnetically levitated Arabidopsis seedlings |
| title_sort | meristematic cell proliferation and ribosome biogenesis are decoupled in diamagnetically levitated arabidopsis seedlings |
| url | https://eprints.nottingham.ac.uk/2757/ https://eprints.nottingham.ac.uk/2757/ https://eprints.nottingham.ac.uk/2757/ |