Kin selection, quorum sensing and virulence in pathogenic bacteria

Bacterial growth and virulence often depends upon the cooperative release of extracellular factors excreted in response to quorum sensing (QS).We carried out an in vivo selection experiment in mice to examine how QS evolves in response to variation in relatedness (strain diversity), and the conseq...

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Main Authors: Rumbaugh, Kendra P., Trivedi, Urvish, Watters, Chase, Burton-Chellew, Maxwell N., Diggle, Stephen P., West, Stuart A.
Format: Article
Published: Royal Society 2012
Online Access:https://eprints.nottingham.ac.uk/2733/
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author Rumbaugh, Kendra P.
Trivedi, Urvish
Watters, Chase
Burton-Chellew, Maxwell N.
Diggle, Stephen P.
West, Stuart A.
author_facet Rumbaugh, Kendra P.
Trivedi, Urvish
Watters, Chase
Burton-Chellew, Maxwell N.
Diggle, Stephen P.
West, Stuart A.
author_sort Rumbaugh, Kendra P.
building Nottingham Research Data Repository
collection Online Access
description Bacterial growth and virulence often depends upon the cooperative release of extracellular factors excreted in response to quorum sensing (QS).We carried out an in vivo selection experiment in mice to examine how QS evolves in response to variation in relatedness (strain diversity), and the consequences for virulence. We started our experiment with two bacterial strains: a wild-type that both produces and responds to QS signal molecules, and a lasR (signal-blind) mutant that does not release extracellular factors in response to signal. We found that: (i) QS leads to greater growth within hosts; (ii) high relatedness favours the QS wild-type; and (iii) low relatedness favours the lasR mutant. Relatedness matters in our experiment because, at relatively low relatedness, the lasR mutant is able to exploit the extracellular factors produced by the cells that respond to QS, and hence increase in frequency. Furthermore, our results suggest that because a higher relatedness favours cooperative QS, and hence leads to higher growth, this will also lead to a higher virulence, giving a relationship between relatedness and virulence that is in the opposite direction to that usually predicted by virulence theory.
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spelling nottingham-27332020-05-04T20:21:24Z https://eprints.nottingham.ac.uk/2733/ Kin selection, quorum sensing and virulence in pathogenic bacteria Rumbaugh, Kendra P. Trivedi, Urvish Watters, Chase Burton-Chellew, Maxwell N. Diggle, Stephen P. West, Stuart A. Bacterial growth and virulence often depends upon the cooperative release of extracellular factors excreted in response to quorum sensing (QS).We carried out an in vivo selection experiment in mice to examine how QS evolves in response to variation in relatedness (strain diversity), and the consequences for virulence. We started our experiment with two bacterial strains: a wild-type that both produces and responds to QS signal molecules, and a lasR (signal-blind) mutant that does not release extracellular factors in response to signal. We found that: (i) QS leads to greater growth within hosts; (ii) high relatedness favours the QS wild-type; and (iii) low relatedness favours the lasR mutant. Relatedness matters in our experiment because, at relatively low relatedness, the lasR mutant is able to exploit the extracellular factors produced by the cells that respond to QS, and hence increase in frequency. Furthermore, our results suggest that because a higher relatedness favours cooperative QS, and hence leads to higher growth, this will also lead to a higher virulence, giving a relationship between relatedness and virulence that is in the opposite direction to that usually predicted by virulence theory. Royal Society 2012-09 Article PeerReviewed Rumbaugh, Kendra P., Trivedi, Urvish, Watters, Chase, Burton-Chellew, Maxwell N., Diggle, Stephen P. and West, Stuart A. (2012) Kin selection, quorum sensing and virulence in pathogenic bacteria. Proceedings of the Royal Society B: Biological Sciences, 279 (1742). pp. 3584-3588. ISSN 0962-8452 http://rspb.royalsocietypublishing.org/content/279/1742/3584.abstract doi:10.1098/rspb.2012.0843 doi:10.1098/rspb.2012.0843
spellingShingle Rumbaugh, Kendra P.
Trivedi, Urvish
Watters, Chase
Burton-Chellew, Maxwell N.
Diggle, Stephen P.
West, Stuart A.
Kin selection, quorum sensing and virulence in pathogenic bacteria
title Kin selection, quorum sensing and virulence in pathogenic bacteria
title_full Kin selection, quorum sensing and virulence in pathogenic bacteria
title_fullStr Kin selection, quorum sensing and virulence in pathogenic bacteria
title_full_unstemmed Kin selection, quorum sensing and virulence in pathogenic bacteria
title_short Kin selection, quorum sensing and virulence in pathogenic bacteria
title_sort kin selection, quorum sensing and virulence in pathogenic bacteria
url https://eprints.nottingham.ac.uk/2733/
https://eprints.nottingham.ac.uk/2733/
https://eprints.nottingham.ac.uk/2733/