5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary
5-Methylcytosine (5-mC) is an epigenetic modification associated with gene repression. Recent studies demonstrated that 5-mC can be enzymatically oxidised into 5-hydroxymethylcytosine and further into 5-formylcytosine (5-fC) and 5-carboxylcytsine (5-caC). 5-caC has been found in embryonic stem cells...
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| Format: | Article |
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Landes Bioscience
2012
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| Online Access: | https://eprints.nottingham.ac.uk/2699/ |
| _version_ | 1848790853007441920 |
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| author | Alioui, Anthony Wheldon, Lee M. Abakir, Abdulkadir Ferjentsik, Zoltan Johnson, Andrew D. Ruzov, Alexey |
| author_facet | Alioui, Anthony Wheldon, Lee M. Abakir, Abdulkadir Ferjentsik, Zoltan Johnson, Andrew D. Ruzov, Alexey |
| author_sort | Alioui, Anthony |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | 5-Methylcytosine (5-mC) is an epigenetic modification associated with gene repression. Recent studies demonstrated that 5-mC can be enzymatically oxidised into 5-hydroxymethylcytosine and further into 5-formylcytosine (5-fC) and 5-carboxylcytsine (5-caC). 5-caC has been found in embryonic stem cells and in mouse pre-implantation embryos but no detectable levels of this modification have been reported for somatic tissues to date. Whereas it has been suggested that 5-caC can serve as an intermediate in the process of active demethylation, the function of this form of modified cytosine remains obscure. Here we show that 5-caC is immunochemically detectable in somatic cells of axolotl ovary. We demonstrate that both 5-hmC and 5-caC are localized to the euchromatin in the nuclei of axolotl follicular cells with similar patterns of spatial distribution. Our results suggest that 5-carboxylcytosine may play a distinct functional role in certain biological contexts. |
| first_indexed | 2025-11-14T18:19:12Z |
| format | Article |
| id | nottingham-2699 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:19:12Z |
| publishDate | 2012 |
| publisher | Landes Bioscience |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-26992020-05-04T20:21:09Z https://eprints.nottingham.ac.uk/2699/ 5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary Alioui, Anthony Wheldon, Lee M. Abakir, Abdulkadir Ferjentsik, Zoltan Johnson, Andrew D. Ruzov, Alexey 5-Methylcytosine (5-mC) is an epigenetic modification associated with gene repression. Recent studies demonstrated that 5-mC can be enzymatically oxidised into 5-hydroxymethylcytosine and further into 5-formylcytosine (5-fC) and 5-carboxylcytsine (5-caC). 5-caC has been found in embryonic stem cells and in mouse pre-implantation embryos but no detectable levels of this modification have been reported for somatic tissues to date. Whereas it has been suggested that 5-caC can serve as an intermediate in the process of active demethylation, the function of this form of modified cytosine remains obscure. Here we show that 5-caC is immunochemically detectable in somatic cells of axolotl ovary. We demonstrate that both 5-hmC and 5-caC are localized to the euchromatin in the nuclei of axolotl follicular cells with similar patterns of spatial distribution. Our results suggest that 5-carboxylcytosine may play a distinct functional role in certain biological contexts. Landes Bioscience 2012-11 Article PeerReviewed Alioui, Anthony, Wheldon, Lee M., Abakir, Abdulkadir, Ferjentsik, Zoltan, Johnson, Andrew D. and Ruzov, Alexey (2012) 5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary. Nucleus, 3 (6). pp. 565-569. ISSN 1949-1034 https://www.landesbioscience.com/journals/nucleus/article/22799/ doi:10.4161/nucl.22799 doi:10.4161/nucl.22799 |
| spellingShingle | Alioui, Anthony Wheldon, Lee M. Abakir, Abdulkadir Ferjentsik, Zoltan Johnson, Andrew D. Ruzov, Alexey 5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary |
| title | 5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary |
| title_full | 5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary |
| title_fullStr | 5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary |
| title_full_unstemmed | 5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary |
| title_short | 5-Carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary |
| title_sort | 5-carboxylcytosine is localized to euchromatic regions in the nuclei of follicular cells in axolotl ovary |
| url | https://eprints.nottingham.ac.uk/2699/ https://eprints.nottingham.ac.uk/2699/ https://eprints.nottingham.ac.uk/2699/ |