Current status of drug screening and disease modelling in human pluripotent stem cells
The emphasis in human pluripotent stem cell (hPSC) technologies has shifted from cell therapy to in vitro disease modelling and drug screening. This review examines why this shift has occurred, and how current technological limitations might be overcome to fully realise the potential of hPSCs. Detai...
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| Format: | Article |
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Wiley-VCH Verlag
2013
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| Online Access: | https://eprints.nottingham.ac.uk/2665/ |
| _version_ | 1848790844024291328 |
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| author | Rajamohan, Divya Matsa, Elena Kalra, Spandan Crutchley, James Patel, Asha George, Vinoj Denning, Chris |
| author_facet | Rajamohan, Divya Matsa, Elena Kalra, Spandan Crutchley, James Patel, Asha George, Vinoj Denning, Chris |
| author_sort | Rajamohan, Divya |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The emphasis in human pluripotent stem cell (hPSC) technologies has shifted from cell therapy to in vitro disease modelling and drug screening. This review examines why this shift has occurred, and how current technological limitations might be overcome to fully realise the potential of hPSCs. Details are provided for all disease-specific human induced pluripotent stem cell lines spanning a dozen dysfunctional organ systems. Phenotype and pharmacology have been examined in only 17 of 63 lines, primarily those that model neurological and cardiac conditions. Drug screening is most advanced in hPSC-cardiomyocytes. Responses for almost 60 agents include examples of how careful tests in hPSC-cardiomyocytes have improved on existing in vitro assays, and how these cells have been integrated into high throughput imaging and electrophysiology industrial platforms. Such successes will provide an incentive to overcome bottlenecks in hPSC technology such as improving cell maturity and industrial scalability whilst reducing cost. |
| first_indexed | 2025-11-14T18:19:04Z |
| format | Article |
| id | nottingham-2665 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:19:04Z |
| publishDate | 2013 |
| publisher | Wiley-VCH Verlag |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-26652020-05-04T20:19:34Z https://eprints.nottingham.ac.uk/2665/ Current status of drug screening and disease modelling in human pluripotent stem cells Rajamohan, Divya Matsa, Elena Kalra, Spandan Crutchley, James Patel, Asha George, Vinoj Denning, Chris The emphasis in human pluripotent stem cell (hPSC) technologies has shifted from cell therapy to in vitro disease modelling and drug screening. This review examines why this shift has occurred, and how current technological limitations might be overcome to fully realise the potential of hPSCs. Details are provided for all disease-specific human induced pluripotent stem cell lines spanning a dozen dysfunctional organ systems. Phenotype and pharmacology have been examined in only 17 of 63 lines, primarily those that model neurological and cardiac conditions. Drug screening is most advanced in hPSC-cardiomyocytes. Responses for almost 60 agents include examples of how careful tests in hPSC-cardiomyocytes have improved on existing in vitro assays, and how these cells have been integrated into high throughput imaging and electrophysiology industrial platforms. Such successes will provide an incentive to overcome bottlenecks in hPSC technology such as improving cell maturity and industrial scalability whilst reducing cost. Wiley-VCH Verlag 2013-03 Article PeerReviewed Rajamohan, Divya, Matsa, Elena, Kalra, Spandan, Crutchley, James, Patel, Asha, George, Vinoj and Denning, Chris (2013) Current status of drug screening and disease modelling in human pluripotent stem cells. BioEssays, 35 (3). pp. 281-298. ISSN 0265-9247 Automation Cardiomyocytes Drug safety assessment Human embryonic stem cells Human induced pluripotent stem cells http://onlinelibrary.wiley.com/doi/10.1002/bies.201200053/abstract doi:10.1002/bies.201200053 doi:10.1002/bies.201200053 |
| spellingShingle | Automation Cardiomyocytes Drug safety assessment Human embryonic stem cells Human induced pluripotent stem cells Rajamohan, Divya Matsa, Elena Kalra, Spandan Crutchley, James Patel, Asha George, Vinoj Denning, Chris Current status of drug screening and disease modelling in human pluripotent stem cells |
| title | Current status of drug screening and disease modelling in human pluripotent stem cells |
| title_full | Current status of drug screening and disease modelling in human pluripotent stem cells |
| title_fullStr | Current status of drug screening and disease modelling in human pluripotent stem cells |
| title_full_unstemmed | Current status of drug screening and disease modelling in human pluripotent stem cells |
| title_short | Current status of drug screening and disease modelling in human pluripotent stem cells |
| title_sort | current status of drug screening and disease modelling in human pluripotent stem cells |
| topic | Automation Cardiomyocytes Drug safety assessment Human embryonic stem cells Human induced pluripotent stem cells |
| url | https://eprints.nottingham.ac.uk/2665/ https://eprints.nottingham.ac.uk/2665/ https://eprints.nottingham.ac.uk/2665/ |