The presence of tumour-associated lymphocytes confers a good prognosis in pancreatic ductal adenocarcinoma: an immunohistochemical study of tissue microarrays
Background Tumour-associated lymphocytes (TALs) have been linked with good prognosis in several solid tumours. This study aimed to evaluate the prognostic significance of CD3, CD8 and CD20 positive lymphocytes in pancreatic ductal adenocarcinoma. Methods After histological re-evaluation of the...
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| Format: | Article |
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BioMed Central
2013
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| Online Access: | https://eprints.nottingham.ac.uk/2645/ |
| _version_ | 1848790838355689472 |
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| author | Tewari, Nilanjana Zaitoun, Abed M. Arora, Arvind Madhusudan, Srinivasan Ilyas, Mohammad Lobo, Dileep N. |
| author_facet | Tewari, Nilanjana Zaitoun, Abed M. Arora, Arvind Madhusudan, Srinivasan Ilyas, Mohammad Lobo, Dileep N. |
| author_sort | Tewari, Nilanjana |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Background
Tumour-associated lymphocytes (TALs) have been linked with good prognosis in several solid tumours. This study aimed to evaluate the prognostic significance of CD3, CD8 and CD20 positive lymphocytes in pancreatic ductal adenocarcinoma.
Methods
After histological re-evaluation of the tumours of 81 patients who underwent surgical resection for exclusively pancreatic ductal adenocarcinoma, tissue micro-arrays (TMA) were constructed and immunohistochemistry was performed for CD3, CD8 and CD20. The number of lymphocytes within specific tumour compartments (i.e. stromal and intratumoural) was quantified. X-tile software (Yale School of Medicine, CT, USA) was used to stratify patients into 'high’ and 'low’ for each of the lymphocytes stained and their association with survival. Receiver operating curves (ROC) were constructed to evaluate the association between the TALs, alone and in combination, with clinicopathological features.
Results
CD3 and CD8 positive lymphocytes were associated with grade of tumour differentiation. The presence of intratumoural CD3 positive cells was associated with improved survival (p = 0.028), and intratumoural and stromal CD3 in combination also correlated with improved survival (p = 0.043). When CD20 positive lymphocyte levels were high, survival improved (p = 0.029) and similar results were seen for CD20 in combination with intratumoural CD3 (p = 0.001) and stromal CD8 (p = 0.013).
Conclusions
This study has shown a correlation between the presence of TALs and survival in pancreatic ductal adenocarcinoma. |
| first_indexed | 2025-11-14T18:18:58Z |
| format | Article |
| id | nottingham-2645 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:18:58Z |
| publishDate | 2013 |
| publisher | BioMed Central |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-26452020-05-04T16:38:43Z https://eprints.nottingham.ac.uk/2645/ The presence of tumour-associated lymphocytes confers a good prognosis in pancreatic ductal adenocarcinoma: an immunohistochemical study of tissue microarrays Tewari, Nilanjana Zaitoun, Abed M. Arora, Arvind Madhusudan, Srinivasan Ilyas, Mohammad Lobo, Dileep N. Background Tumour-associated lymphocytes (TALs) have been linked with good prognosis in several solid tumours. This study aimed to evaluate the prognostic significance of CD3, CD8 and CD20 positive lymphocytes in pancreatic ductal adenocarcinoma. Methods After histological re-evaluation of the tumours of 81 patients who underwent surgical resection for exclusively pancreatic ductal adenocarcinoma, tissue micro-arrays (TMA) were constructed and immunohistochemistry was performed for CD3, CD8 and CD20. The number of lymphocytes within specific tumour compartments (i.e. stromal and intratumoural) was quantified. X-tile software (Yale School of Medicine, CT, USA) was used to stratify patients into 'high’ and 'low’ for each of the lymphocytes stained and their association with survival. Receiver operating curves (ROC) were constructed to evaluate the association between the TALs, alone and in combination, with clinicopathological features. Results CD3 and CD8 positive lymphocytes were associated with grade of tumour differentiation. The presence of intratumoural CD3 positive cells was associated with improved survival (p = 0.028), and intratumoural and stromal CD3 in combination also correlated with improved survival (p = 0.043). When CD20 positive lymphocyte levels were high, survival improved (p = 0.029) and similar results were seen for CD20 in combination with intratumoural CD3 (p = 0.001) and stromal CD8 (p = 0.013). Conclusions This study has shown a correlation between the presence of TALs and survival in pancreatic ductal adenocarcinoma. BioMed Central 2013-09-24 Article PeerReviewed Tewari, Nilanjana, Zaitoun, Abed M., Arora, Arvind, Madhusudan, Srinivasan, Ilyas, Mohammad and Lobo, Dileep N. (2013) The presence of tumour-associated lymphocytes confers a good prognosis in pancreatic ductal adenocarcinoma: an immunohistochemical study of tissue microarrays. BMC Cancer, 13 . 436/1-436/10. ISSN 1471-2407 CD3; CD8; Pancreatic ductal adenocarcinoma; Tumour-associated lymphocytes; Prognosis; Immunohistochemistry http://www.biomedcentral.com/1471-2407/13/436 doi:10.1186/1471-2407-13-436 doi:10.1186/1471-2407-13-436 |
| spellingShingle | CD3; CD8; Pancreatic ductal adenocarcinoma; Tumour-associated lymphocytes; Prognosis; Immunohistochemistry Tewari, Nilanjana Zaitoun, Abed M. Arora, Arvind Madhusudan, Srinivasan Ilyas, Mohammad Lobo, Dileep N. The presence of tumour-associated lymphocytes confers a good prognosis in pancreatic ductal adenocarcinoma: an immunohistochemical study of tissue microarrays |
| title | The presence of tumour-associated lymphocytes confers a good prognosis in pancreatic ductal adenocarcinoma: an immunohistochemical study of tissue microarrays |
| title_full | The presence of tumour-associated lymphocytes confers a good prognosis in pancreatic ductal adenocarcinoma: an immunohistochemical study of tissue microarrays |
| title_fullStr | The presence of tumour-associated lymphocytes confers a good prognosis in pancreatic ductal adenocarcinoma: an immunohistochemical study of tissue microarrays |
| title_full_unstemmed | The presence of tumour-associated lymphocytes confers a good prognosis in pancreatic ductal adenocarcinoma: an immunohistochemical study of tissue microarrays |
| title_short | The presence of tumour-associated lymphocytes confers a good prognosis in pancreatic ductal adenocarcinoma: an immunohistochemical study of tissue microarrays |
| title_sort | presence of tumour-associated lymphocytes confers a good prognosis in pancreatic ductal adenocarcinoma: an immunohistochemical study of tissue microarrays |
| topic | CD3; CD8; Pancreatic ductal adenocarcinoma; Tumour-associated lymphocytes; Prognosis; Immunohistochemistry |
| url | https://eprints.nottingham.ac.uk/2645/ https://eprints.nottingham.ac.uk/2645/ https://eprints.nottingham.ac.uk/2645/ |