Effects of α-synuclein overexpression in transgenic Caenorhabditis elegans strains
The neural protein α-synuclein aggregates both in vivo and in vitro to form insoluble fibrils that are involved in Parkinson’s disease pathogenesis. We have generated α-synuclein/fluorescent-protein fusion constructs overexpressed in muscle cells of the nematode, Caenorhabdtis elegans. Green Fluore...
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| Format: | Article |
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Bentham Science Publishers
2012
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| Online Access: | https://eprints.nottingham.ac.uk/2591/ |
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| author | Bodhicharla, Rakesh Nagarajan, Archana Winter, Jody Adenle, Ademola Nazir, Aamir Brady, Declan Vere, Kelly Richens, Jo O'Shea, Paul Bell, David R. de Pomerai, David I. |
| author_facet | Bodhicharla, Rakesh Nagarajan, Archana Winter, Jody Adenle, Ademola Nazir, Aamir Brady, Declan Vere, Kelly Richens, Jo O'Shea, Paul Bell, David R. de Pomerai, David I. |
| author_sort | Bodhicharla, Rakesh |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The neural protein α-synuclein aggregates both in vivo and in vitro to form insoluble fibrils that are involved in
Parkinson’s disease pathogenesis. We have generated α-synuclein/fluorescent-protein fusion constructs overexpressed in muscle cells of the nematode, Caenorhabdtis elegans. Green Fluorescent Protein (GFP) variants, Cerulean (C) or Venus (V), were fused to the C-terminus of human α-synuclein (S); the resultant fusion genes were designated SV and SC, plus a CV fusion as well as S, C and V singly. The aggregation behavior of the purified fusion proteins (expressed in E. coli) will be
described elsewhere. These constructs were fused to a C. elegans unc-54 myosin promoter, and integrated transgenic lines generated by microinjection, gamma-irradiation, and outcrossing of fluorescent progeny. All transgenic lines expressing α-synuclein showed significant reductions (p < 0.05) in lifespan, motility and pharyngeal pumping, as compared to wildtype worms or lines expressing CFP and/or YFP only. We showed that CFP and YFP labels colocalised in granular inclusions throughout the body wall in transgenic lines expressing both SC and SV fusions (SC+SV), whereas SV+C worms. |
| first_indexed | 2025-11-14T18:18:46Z |
| format | Article |
| id | nottingham-2591 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:18:46Z |
| publishDate | 2012 |
| publisher | Bentham Science Publishers |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-25912020-05-04T20:22:01Z https://eprints.nottingham.ac.uk/2591/ Effects of α-synuclein overexpression in transgenic Caenorhabditis elegans strains Bodhicharla, Rakesh Nagarajan, Archana Winter, Jody Adenle, Ademola Nazir, Aamir Brady, Declan Vere, Kelly Richens, Jo O'Shea, Paul Bell, David R. de Pomerai, David I. The neural protein α-synuclein aggregates both in vivo and in vitro to form insoluble fibrils that are involved in Parkinson’s disease pathogenesis. We have generated α-synuclein/fluorescent-protein fusion constructs overexpressed in muscle cells of the nematode, Caenorhabdtis elegans. Green Fluorescent Protein (GFP) variants, Cerulean (C) or Venus (V), were fused to the C-terminus of human α-synuclein (S); the resultant fusion genes were designated SV and SC, plus a CV fusion as well as S, C and V singly. The aggregation behavior of the purified fusion proteins (expressed in E. coli) will be described elsewhere. These constructs were fused to a C. elegans unc-54 myosin promoter, and integrated transgenic lines generated by microinjection, gamma-irradiation, and outcrossing of fluorescent progeny. All transgenic lines expressing α-synuclein showed significant reductions (p < 0.05) in lifespan, motility and pharyngeal pumping, as compared to wildtype worms or lines expressing CFP and/or YFP only. We showed that CFP and YFP labels colocalised in granular inclusions throughout the body wall in transgenic lines expressing both SC and SV fusions (SC+SV), whereas SV+C worms. Bentham Science Publishers 2012 Article PeerReviewed Bodhicharla, Rakesh, Nagarajan, Archana, Winter, Jody, Adenle, Ademola, Nazir, Aamir, Brady, Declan, Vere, Kelly, Richens, Jo, O'Shea, Paul, Bell, David R. and de Pomerai, David I. (2012) Effects of α-synuclein overexpression in transgenic Caenorhabditis elegans strains. CNS and Neurological Disorders - Drug Targets, 11 (8). pp. 965-975. ISSN 1871-5273 Caenorhabditis elegans CFP and YFP reporters FRET protein aggregation α-synuclein transgenic strains http://europepmc.org/articles/PMC3744922/reload=0;jsessionid=JPd76GgNT9LPI7BNrhZt.12 doi:10.2174/1871527311211080005 doi:10.2174/1871527311211080005 |
| spellingShingle | Caenorhabditis elegans CFP and YFP reporters FRET protein aggregation α-synuclein transgenic strains Bodhicharla, Rakesh Nagarajan, Archana Winter, Jody Adenle, Ademola Nazir, Aamir Brady, Declan Vere, Kelly Richens, Jo O'Shea, Paul Bell, David R. de Pomerai, David I. Effects of α-synuclein overexpression in transgenic Caenorhabditis elegans strains |
| title | Effects of α-synuclein overexpression in transgenic Caenorhabditis elegans strains |
| title_full | Effects of α-synuclein overexpression in transgenic Caenorhabditis elegans strains |
| title_fullStr | Effects of α-synuclein overexpression in transgenic Caenorhabditis elegans strains |
| title_full_unstemmed | Effects of α-synuclein overexpression in transgenic Caenorhabditis elegans strains |
| title_short | Effects of α-synuclein overexpression in transgenic Caenorhabditis elegans strains |
| title_sort | effects of α-synuclein overexpression in transgenic caenorhabditis elegans strains |
| topic | Caenorhabditis elegans CFP and YFP reporters FRET protein aggregation α-synuclein transgenic strains |
| url | https://eprints.nottingham.ac.uk/2591/ https://eprints.nottingham.ac.uk/2591/ https://eprints.nottingham.ac.uk/2591/ |