Endogenous retrovirus EAV-HP linked to blue egg phenotype in Mapuche fowl

Oocyan or blue/green eggshell colour is an autosomal dominant trait found in native chickens (Mapuche fowl) of Chile and in some of their descendants in European and North American modern breeds. We report here the identification of an endogenous avian retroviral (EAV-HP) insertion in oocyan Mapuche...

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Main Authors: Wragg, David, Mwacharo, Joram M., Alcalde, José A., Wang, Chen, Han, Jian-Lin, Gongora, Jaime, Gourichon, David, Tixier-Boichard, Michèle, Hanotte, Olivier
Format: Article
Published: Public Library of Science 2013
Online Access:https://eprints.nottingham.ac.uk/2534/
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author Wragg, David
Mwacharo, Joram M.
Alcalde, José A.
Wang, Chen
Han, Jian-Lin
Gongora, Jaime
Gourichon, David
Tixier-Boichard, Michèle
Hanotte, Olivier
author_facet Wragg, David
Mwacharo, Joram M.
Alcalde, José A.
Wang, Chen
Han, Jian-Lin
Gongora, Jaime
Gourichon, David
Tixier-Boichard, Michèle
Hanotte, Olivier
author_sort Wragg, David
building Nottingham Research Data Repository
collection Online Access
description Oocyan or blue/green eggshell colour is an autosomal dominant trait found in native chickens (Mapuche fowl) of Chile and in some of their descendants in European and North American modern breeds. We report here the identification of an endogenous avian retroviral (EAV-HP) insertion in oocyan Mapuche fowl and European breeds. Sequencing data reveals 100% retroviral identity between the Mapuche and European insertions. Quantitative real-time PCR analysis of European oocyan chicken indicates over-expression of the SLCO1B3 gene (P<0.05) in the shell gland and oviduct. Predicted transcription factor binding sites in the long terminal repeats (LTR) indicate AhR/Ar, a modulator of oestrogen, as a possible promoter/enhancer leading to reproductive tissue-specific over-expression of the SLCO1B3 gene. Analysis of all jungle fowl species Gallus sp. supports the retroviral insertion to be a post-domestication event, while identical LTR sequences within domestic chickens are in agreement with a recent de novo mutation.
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spelling nottingham-25342024-08-15T15:14:23Z https://eprints.nottingham.ac.uk/2534/ Endogenous retrovirus EAV-HP linked to blue egg phenotype in Mapuche fowl Wragg, David Mwacharo, Joram M. Alcalde, José A. Wang, Chen Han, Jian-Lin Gongora, Jaime Gourichon, David Tixier-Boichard, Michèle Hanotte, Olivier Oocyan or blue/green eggshell colour is an autosomal dominant trait found in native chickens (Mapuche fowl) of Chile and in some of their descendants in European and North American modern breeds. We report here the identification of an endogenous avian retroviral (EAV-HP) insertion in oocyan Mapuche fowl and European breeds. Sequencing data reveals 100% retroviral identity between the Mapuche and European insertions. Quantitative real-time PCR analysis of European oocyan chicken indicates over-expression of the SLCO1B3 gene (P<0.05) in the shell gland and oviduct. Predicted transcription factor binding sites in the long terminal repeats (LTR) indicate AhR/Ar, a modulator of oestrogen, as a possible promoter/enhancer leading to reproductive tissue-specific over-expression of the SLCO1B3 gene. Analysis of all jungle fowl species Gallus sp. supports the retroviral insertion to be a post-domestication event, while identical LTR sequences within domestic chickens are in agreement with a recent de novo mutation. Public Library of Science 2013-08-19 Article PeerReviewed Wragg, David, Mwacharo, Joram M., Alcalde, José A., Wang, Chen, Han, Jian-Lin, Gongora, Jaime, Gourichon, David, Tixier-Boichard, Michèle and Hanotte, Olivier (2013) Endogenous retrovirus EAV-HP linked to blue egg phenotype in Mapuche fowl. PLoS ONE, 8 (8). 9/1-9/9. ISSN 1932-6203 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0071393 doi:10.1371/journal.pone.0071393 doi:10.1371/journal.pone.0071393
spellingShingle Wragg, David
Mwacharo, Joram M.
Alcalde, José A.
Wang, Chen
Han, Jian-Lin
Gongora, Jaime
Gourichon, David
Tixier-Boichard, Michèle
Hanotte, Olivier
Endogenous retrovirus EAV-HP linked to blue egg phenotype in Mapuche fowl
title Endogenous retrovirus EAV-HP linked to blue egg phenotype in Mapuche fowl
title_full Endogenous retrovirus EAV-HP linked to blue egg phenotype in Mapuche fowl
title_fullStr Endogenous retrovirus EAV-HP linked to blue egg phenotype in Mapuche fowl
title_full_unstemmed Endogenous retrovirus EAV-HP linked to blue egg phenotype in Mapuche fowl
title_short Endogenous retrovirus EAV-HP linked to blue egg phenotype in Mapuche fowl
title_sort endogenous retrovirus eav-hp linked to blue egg phenotype in mapuche fowl
url https://eprints.nottingham.ac.uk/2534/
https://eprints.nottingham.ac.uk/2534/
https://eprints.nottingham.ac.uk/2534/