Lipidomic analysis of serum from horses with strongyle infection
The development of techniques capable of accurate diagnosis of strongyle infections is at the forefront of research in equine parasitology. The aim of this study was to evaluate the potential, for using lipidomics in the diagnosis of strongyle infection. Blood and fecal samples were collected from 3...
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| Format: | Article |
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OMICS Publishing Group
2012
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| Online Access: | https://eprints.nottingham.ac.uk/2533/ |
| _version_ | 1848790809937182720 |
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| author | Lamb, Jenna Hallowell, Gayle D. Harris, Patricia Xing-Quan, Zhu Elsheikha, Hany M. |
| author_facet | Lamb, Jenna Hallowell, Gayle D. Harris, Patricia Xing-Quan, Zhu Elsheikha, Hany M. |
| author_sort | Lamb, Jenna |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The development of techniques capable of accurate diagnosis of strongyle infections is at the forefront of research in equine parasitology. The aim of this study was to evaluate the potential, for using lipidomics in the diagnosis of strongyle infection. Blood and fecal samples were collected from 30 horses. Fecal egg count (FEC) results were used to select the serum samples from six uninfected horses (negative controls) and from the five horses with the highest burdens. The lipid portion of serum samples was extracted and analyzed using High Performance Liquid Chromatography-Mass Spectroscopy. The concentrations of 66 lipid metabolites differed between infected and uninfected horses (p<0.025). Database comparison of mass/charge (m/z) ratios and retention times were used to tentatively identify 16 of these metabolites. The roles of these metabolites and reasons for the observable changes were discussed. These results demonstrate the potential for the use of high resolution lipidomic analysis, for the development of a diagnostic technique capable of detecting, and perhaps stratifying, equine strongyle infection. |
| first_indexed | 2025-11-14T18:18:31Z |
| format | Article |
| id | nottingham-2533 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:18:31Z |
| publishDate | 2012 |
| publisher | OMICS Publishing Group |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-25332024-08-15T15:13:49Z https://eprints.nottingham.ac.uk/2533/ Lipidomic analysis of serum from horses with strongyle infection Lamb, Jenna Hallowell, Gayle D. Harris, Patricia Xing-Quan, Zhu Elsheikha, Hany M. The development of techniques capable of accurate diagnosis of strongyle infections is at the forefront of research in equine parasitology. The aim of this study was to evaluate the potential, for using lipidomics in the diagnosis of strongyle infection. Blood and fecal samples were collected from 30 horses. Fecal egg count (FEC) results were used to select the serum samples from six uninfected horses (negative controls) and from the five horses with the highest burdens. The lipid portion of serum samples was extracted and analyzed using High Performance Liquid Chromatography-Mass Spectroscopy. The concentrations of 66 lipid metabolites differed between infected and uninfected horses (p<0.025). Database comparison of mass/charge (m/z) ratios and retention times were used to tentatively identify 16 of these metabolites. The roles of these metabolites and reasons for the observable changes were discussed. These results demonstrate the potential for the use of high resolution lipidomic analysis, for the development of a diagnostic technique capable of detecting, and perhaps stratifying, equine strongyle infection. OMICS Publishing Group 2012-09-20 Article PeerReviewed Lamb, Jenna, Hallowell, Gayle D., Harris, Patricia, Xing-Quan, Zhu and Elsheikha, Hany M. (2012) Lipidomic analysis of serum from horses with strongyle infection. Journal of Veterinary Science and Technology, 3 (6). 122/1-122/4. ISSN 2157-7579 http://omicsonline.org/2157-7579/2157-7579-3-122.digital/2157-7579-3-122.html doi:10.4172/2157-7579.1000122 doi:10.4172/2157-7579.1000122 |
| spellingShingle | Lamb, Jenna Hallowell, Gayle D. Harris, Patricia Xing-Quan, Zhu Elsheikha, Hany M. Lipidomic analysis of serum from horses with strongyle infection |
| title | Lipidomic analysis of serum from horses with strongyle infection |
| title_full | Lipidomic analysis of serum from horses with strongyle infection |
| title_fullStr | Lipidomic analysis of serum from horses with strongyle infection |
| title_full_unstemmed | Lipidomic analysis of serum from horses with strongyle infection |
| title_short | Lipidomic analysis of serum from horses with strongyle infection |
| title_sort | lipidomic analysis of serum from horses with strongyle infection |
| url | https://eprints.nottingham.ac.uk/2533/ https://eprints.nottingham.ac.uk/2533/ https://eprints.nottingham.ac.uk/2533/ |