Botanical extracts from rosehip (rosa canina), willow bark (salix alba), and nettle leaf (urtica dioica) suppress IL-1β-induced NF-κB activation in canine articular chondrocytes
The aim of this study was to characterize the anti-inflammatory mode of action of botanical extracts from rosehip (Rosa canina), willow bark (Salix alba), and nettle leaf (Urtica dioica) in an in vitro model of primary canine articular chondrocytes. Methods. The biological effects of the botanical e...
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| Format: | Article |
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Hindawi Publishing Corporation
2012
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| Online Access: | https://eprints.nottingham.ac.uk/2513/ |
| _version_ | 1848790804193083392 |
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| author | Shakibaei, Mehdi Allaway, David Nebrich, Simone Mobasheri, Ali |
| author_facet | Shakibaei, Mehdi Allaway, David Nebrich, Simone Mobasheri, Ali |
| author_sort | Shakibaei, Mehdi |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The aim of this study was to characterize the anti-inflammatory mode of action of botanical extracts from rosehip (Rosa canina), willow bark (Salix alba), and nettle leaf (Urtica dioica) in an in vitro model of primary canine articular chondrocytes. Methods. The biological effects of the botanical extracts were studied in chondrocytes treated with IL-1β for up to 72 h. Expression of collagen type II, cartilage-specific proteoglycan (CSPG), β1-integrin, SOX-9, COX-2, and MMP-9 and MMP-13 was examined by western blotting. Results. The botanical extracts suppressed IL-1β-induced NF-κB activation by inhibition of IκBα phosphorylation, IκBα degradation, p65 phosphorylation, and p65 nuclear translocation. These events correlated with downregulation of NF-κB targets including COX-2 and MMPs. The extracts also reversed the IL-1β-induced downregulation of collagen type II, CSPG, β1-integrin, and cartilage-specific transcription factor SOX-9 protein expression. In high-density cultures botanical extracts stimulated new cartilage formation even in the presence of IL-1β. Conclusions. Botanical extracts exerted anti-inflammatory and anabolic effects on chondrocytes. The observed reduction of IL-1β-induced NF-κB activation suggests that further studies are warranted to demonstrate the effectiveness of plant extracts in the treatment of OA and other conditions in which NF-κB plays pathophysiological roles. |
| first_indexed | 2025-11-14T18:18:26Z |
| format | Article |
| id | nottingham-2513 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:18:26Z |
| publishDate | 2012 |
| publisher | Hindawi Publishing Corporation |
| recordtype | eprints |
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| spelling | nottingham-25132020-05-04T20:22:50Z https://eprints.nottingham.ac.uk/2513/ Botanical extracts from rosehip (rosa canina), willow bark (salix alba), and nettle leaf (urtica dioica) suppress IL-1β-induced NF-κB activation in canine articular chondrocytes Shakibaei, Mehdi Allaway, David Nebrich, Simone Mobasheri, Ali The aim of this study was to characterize the anti-inflammatory mode of action of botanical extracts from rosehip (Rosa canina), willow bark (Salix alba), and nettle leaf (Urtica dioica) in an in vitro model of primary canine articular chondrocytes. Methods. The biological effects of the botanical extracts were studied in chondrocytes treated with IL-1β for up to 72 h. Expression of collagen type II, cartilage-specific proteoglycan (CSPG), β1-integrin, SOX-9, COX-2, and MMP-9 and MMP-13 was examined by western blotting. Results. The botanical extracts suppressed IL-1β-induced NF-κB activation by inhibition of IκBα phosphorylation, IκBα degradation, p65 phosphorylation, and p65 nuclear translocation. These events correlated with downregulation of NF-κB targets including COX-2 and MMPs. The extracts also reversed the IL-1β-induced downregulation of collagen type II, CSPG, β1-integrin, and cartilage-specific transcription factor SOX-9 protein expression. In high-density cultures botanical extracts stimulated new cartilage formation even in the presence of IL-1β. Conclusions. Botanical extracts exerted anti-inflammatory and anabolic effects on chondrocytes. The observed reduction of IL-1β-induced NF-κB activation suggests that further studies are warranted to demonstrate the effectiveness of plant extracts in the treatment of OA and other conditions in which NF-κB plays pathophysiological roles. Hindawi Publishing Corporation 2012 Article PeerReviewed Shakibaei, Mehdi, Allaway, David, Nebrich, Simone and Mobasheri, Ali (2012) Botanical extracts from rosehip (rosa canina), willow bark (salix alba), and nettle leaf (urtica dioica) suppress IL-1β-induced NF-κB activation in canine articular chondrocytes. Evidence-Based Complementary and Alternative Medicine, 2012 . 16/1-16/16. ISSN 1741-427X http://www.hindawi.com/journals/ecam/2012/509383/ doi:10.1155/2012/509383 doi:10.1155/2012/509383 |
| spellingShingle | Shakibaei, Mehdi Allaway, David Nebrich, Simone Mobasheri, Ali Botanical extracts from rosehip (rosa canina), willow bark (salix alba), and nettle leaf (urtica dioica) suppress IL-1β-induced NF-κB activation in canine articular chondrocytes |
| title | Botanical extracts from rosehip (rosa canina),
willow bark (salix alba), and nettle leaf (urtica dioica)
suppress IL-1β-induced NF-κB activation in
canine articular chondrocytes |
| title_full | Botanical extracts from rosehip (rosa canina),
willow bark (salix alba), and nettle leaf (urtica dioica)
suppress IL-1β-induced NF-κB activation in
canine articular chondrocytes |
| title_fullStr | Botanical extracts from rosehip (rosa canina),
willow bark (salix alba), and nettle leaf (urtica dioica)
suppress IL-1β-induced NF-κB activation in
canine articular chondrocytes |
| title_full_unstemmed | Botanical extracts from rosehip (rosa canina),
willow bark (salix alba), and nettle leaf (urtica dioica)
suppress IL-1β-induced NF-κB activation in
canine articular chondrocytes |
| title_short | Botanical extracts from rosehip (rosa canina),
willow bark (salix alba), and nettle leaf (urtica dioica)
suppress IL-1β-induced NF-κB activation in
canine articular chondrocytes |
| title_sort | botanical extracts from rosehip (rosa canina),
willow bark (salix alba), and nettle leaf (urtica dioica)
suppress il-1β-induced nf-κb activation in
canine articular chondrocytes |
| url | https://eprints.nottingham.ac.uk/2513/ https://eprints.nottingham.ac.uk/2513/ https://eprints.nottingham.ac.uk/2513/ |