Dynamics of DNA replication in yeast

We present a mathematical model for the spatial dynamics of DNA replication. Using this model we determine the probability distribution for the time at which each chromosomal position is replicated. From this we show, contrary to previous reports, that mean replication time curves cannot be used to...

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Main Authors: Retkute, Renata, Nieduszynski, Conrad A., Moura, Alessandro de
Format: Article
Published: American Physical Society 2011
Online Access:https://eprints.nottingham.ac.uk/2492/
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author Retkute, Renata
Nieduszynski, Conrad A.
Moura, Alessandro de
author_facet Retkute, Renata
Nieduszynski, Conrad A.
Moura, Alessandro de
author_sort Retkute, Renata
building Nottingham Research Data Repository
collection Online Access
description We present a mathematical model for the spatial dynamics of DNA replication. Using this model we determine the probability distribution for the time at which each chromosomal position is replicated. From this we show, contrary to previous reports, that mean replication time curves cannot be used to directly determine origin parameters. We demonstrate that the stochastic nature of replication dynamics leaves a clear signature in experimentally measured population average data, and we show that the width of the activation time probability distribution can be inferred from this data. Our results compare favorably with experimental measurements in Saccharomyces cerevisae.
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spelling nottingham-24922020-05-04T20:23:11Z https://eprints.nottingham.ac.uk/2492/ Dynamics of DNA replication in yeast Retkute, Renata Nieduszynski, Conrad A. Moura, Alessandro de We present a mathematical model for the spatial dynamics of DNA replication. Using this model we determine the probability distribution for the time at which each chromosomal position is replicated. From this we show, contrary to previous reports, that mean replication time curves cannot be used to directly determine origin parameters. We demonstrate that the stochastic nature of replication dynamics leaves a clear signature in experimentally measured population average data, and we show that the width of the activation time probability distribution can be inferred from this data. Our results compare favorably with experimental measurements in Saccharomyces cerevisae. American Physical Society 2011-08 Article PeerReviewed Retkute, Renata, Nieduszynski, Conrad A. and Moura, Alessandro de (2011) Dynamics of DNA replication in yeast. Physical Review Letters, 107 . 068103/1-068103/4. ISSN 0031-9007 http://journals.aps.org/prl/abstract/10.1103/PhysRevLett.107.068103 doi:10.1103/PhysRevLett.107.068103 doi:10.1103/PhysRevLett.107.068103
spellingShingle Retkute, Renata
Nieduszynski, Conrad A.
Moura, Alessandro de
Dynamics of DNA replication in yeast
title Dynamics of DNA replication in yeast
title_full Dynamics of DNA replication in yeast
title_fullStr Dynamics of DNA replication in yeast
title_full_unstemmed Dynamics of DNA replication in yeast
title_short Dynamics of DNA replication in yeast
title_sort dynamics of dna replication in yeast
url https://eprints.nottingham.ac.uk/2492/
https://eprints.nottingham.ac.uk/2492/
https://eprints.nottingham.ac.uk/2492/