Use of short-tandem repeat (STR) fingerprinting to validate sample origins in hepatitis C virus molecular epidemiology studies
Sequence analysis is used to define the molecular epidemiology and evolution of the hepatitis C virus. Whilst most studies have shown that individual patients harbour viruses that are derived from a limited number of highly related strains, some recent reports have shown that some patients can be co...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Published: |
Society for General Microbiology
2014
|
| Online Access: | https://eprints.nottingham.ac.uk/2390/ |
| _version_ | 1848790772864778240 |
|---|---|
| author | Edwards, Victoria C. McClure, Patrick Brown, Richard J.P. Thompson, Emma Irving, William L. Ball, Jonathan K. |
| author_facet | Edwards, Victoria C. McClure, Patrick Brown, Richard J.P. Thompson, Emma Irving, William L. Ball, Jonathan K. |
| author_sort | Edwards, Victoria C. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Sequence analysis is used to define the molecular epidemiology and evolution of the hepatitis C virus. Whilst most studies have shown that individual patients harbour viruses that are derived from a limited number of highly related strains, some recent reports have shown that some patients can be co-infected with very distinct variants whose frequency can fluctuate greatly. Whilst co-infection with highly divergent strains is possible, an alternative explanation is that such data represent contamination or sample mix-up. In this study, we have shown that DNA fingerprinting techniques can accurately assess sample provenance and differentiate between samples that are truly exhibiting mixed infection from those that harbour distinct virus populations due to sample mix-up. We have argued that this approach should be adopted routinely in virus sequence analyses to validate sample provenance. |
| first_indexed | 2025-11-14T18:17:56Z |
| format | Article |
| id | nottingham-2390 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:17:56Z |
| publishDate | 2014 |
| publisher | Society for General Microbiology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-23902020-05-04T16:40:11Z https://eprints.nottingham.ac.uk/2390/ Use of short-tandem repeat (STR) fingerprinting to validate sample origins in hepatitis C virus molecular epidemiology studies Edwards, Victoria C. McClure, Patrick Brown, Richard J.P. Thompson, Emma Irving, William L. Ball, Jonathan K. Sequence analysis is used to define the molecular epidemiology and evolution of the hepatitis C virus. Whilst most studies have shown that individual patients harbour viruses that are derived from a limited number of highly related strains, some recent reports have shown that some patients can be co-infected with very distinct variants whose frequency can fluctuate greatly. Whilst co-infection with highly divergent strains is possible, an alternative explanation is that such data represent contamination or sample mix-up. In this study, we have shown that DNA fingerprinting techniques can accurately assess sample provenance and differentiate between samples that are truly exhibiting mixed infection from those that harbour distinct virus populations due to sample mix-up. We have argued that this approach should be adopted routinely in virus sequence analyses to validate sample provenance. Society for General Microbiology 2014-01-01 Article PeerReviewed Edwards, Victoria C., McClure, Patrick, Brown, Richard J.P., Thompson, Emma, Irving, William L. and Ball, Jonathan K. (2014) Use of short-tandem repeat (STR) fingerprinting to validate sample origins in hepatitis C virus molecular epidemiology studies. Journal of General Virology, 95 (1). pp. 66-70. ISSN 1465-2099 http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.057828-0 doi:10.1099/vir.0.057828-0 doi:10.1099/vir.0.057828-0 |
| spellingShingle | Edwards, Victoria C. McClure, Patrick Brown, Richard J.P. Thompson, Emma Irving, William L. Ball, Jonathan K. Use of short-tandem repeat (STR) fingerprinting to validate sample origins in hepatitis C virus molecular epidemiology studies |
| title | Use of short-tandem repeat (STR) fingerprinting to validate sample origins in hepatitis C virus molecular epidemiology studies |
| title_full | Use of short-tandem repeat (STR) fingerprinting to validate sample origins in hepatitis C virus molecular epidemiology studies |
| title_fullStr | Use of short-tandem repeat (STR) fingerprinting to validate sample origins in hepatitis C virus molecular epidemiology studies |
| title_full_unstemmed | Use of short-tandem repeat (STR) fingerprinting to validate sample origins in hepatitis C virus molecular epidemiology studies |
| title_short | Use of short-tandem repeat (STR) fingerprinting to validate sample origins in hepatitis C virus molecular epidemiology studies |
| title_sort | use of short-tandem repeat (str) fingerprinting to validate sample origins in hepatitis c virus molecular epidemiology studies |
| url | https://eprints.nottingham.ac.uk/2390/ https://eprints.nottingham.ac.uk/2390/ https://eprints.nottingham.ac.uk/2390/ |