Bursting the bubble on bacterial biofilms: a flow cell methodology

The flow cell biofilm system is an important and widely used tool for the in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow. This paper provides an introduction to the background and use of such systems, accompanied by a detailed guide to the assembly of...

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Main Authors: Crusz, Shanika A., Popat, Roman, Rybtke, Morten Theil, Cámara, Miguel, Givskov, Michael, Tolker-Nielsen, Tim, Diggle, Stephen P., Williams, Paul
Format: Article
Published: Taylor & Francis 2012
Online Access:https://eprints.nottingham.ac.uk/2319/
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author Crusz, Shanika A.
Popat, Roman
Rybtke, Morten Theil
Cámara, Miguel
Givskov, Michael
Tolker-Nielsen, Tim
Diggle, Stephen P.
Williams, Paul
author_facet Crusz, Shanika A.
Popat, Roman
Rybtke, Morten Theil
Cámara, Miguel
Givskov, Michael
Tolker-Nielsen, Tim
Diggle, Stephen P.
Williams, Paul
author_sort Crusz, Shanika A.
building Nottingham Research Data Repository
collection Online Access
description The flow cell biofilm system is an important and widely used tool for the in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow. This paper provides an introduction to the background and use of such systems, accompanied by a detailed guide to the assembly of the apparatus including the description of new modifications which enhance its performance. As such, this is an essential guide for the novice biofilm researcher as well as providing valuable trouble-shooting techniques for even the most experienced laboratories. The adoption of a common and reliable methodology amongst researchers would enable findings to be shared and replicated amongst the biofilm research community, with the overall aim of advancing understanding and management of these complex and widespread bacterial communities.
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publishDate 2012
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spelling nottingham-23192020-05-04T20:22:22Z https://eprints.nottingham.ac.uk/2319/ Bursting the bubble on bacterial biofilms: a flow cell methodology Crusz, Shanika A. Popat, Roman Rybtke, Morten Theil Cámara, Miguel Givskov, Michael Tolker-Nielsen, Tim Diggle, Stephen P. Williams, Paul The flow cell biofilm system is an important and widely used tool for the in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow. This paper provides an introduction to the background and use of such systems, accompanied by a detailed guide to the assembly of the apparatus including the description of new modifications which enhance its performance. As such, this is an essential guide for the novice biofilm researcher as well as providing valuable trouble-shooting techniques for even the most experienced laboratories. The adoption of a common and reliable methodology amongst researchers would enable findings to be shared and replicated amongst the biofilm research community, with the overall aim of advancing understanding and management of these complex and widespread bacterial communities. Taylor & Francis 2012 Article PeerReviewed Crusz, Shanika A., Popat, Roman, Rybtke, Morten Theil, Cámara, Miguel, Givskov, Michael, Tolker-Nielsen, Tim, Diggle, Stephen P. and Williams, Paul (2012) Bursting the bubble on bacterial biofilms: a flow cell methodology. Biofouling, 28 (8). pp. 835-842. ISSN 0892-7014 http://www.tandfonline.com/doi/full/10.1080/08927014.2012.716044 doi:10.1080/08927014.2012.716044 doi:10.1080/08927014.2012.716044
spellingShingle Crusz, Shanika A.
Popat, Roman
Rybtke, Morten Theil
Cámara, Miguel
Givskov, Michael
Tolker-Nielsen, Tim
Diggle, Stephen P.
Williams, Paul
Bursting the bubble on bacterial biofilms: a flow cell methodology
title Bursting the bubble on bacterial biofilms: a flow cell methodology
title_full Bursting the bubble on bacterial biofilms: a flow cell methodology
title_fullStr Bursting the bubble on bacterial biofilms: a flow cell methodology
title_full_unstemmed Bursting the bubble on bacterial biofilms: a flow cell methodology
title_short Bursting the bubble on bacterial biofilms: a flow cell methodology
title_sort bursting the bubble on bacterial biofilms: a flow cell methodology
url https://eprints.nottingham.ac.uk/2319/
https://eprints.nottingham.ac.uk/2319/
https://eprints.nottingham.ac.uk/2319/