Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite
Dendritic cells (DCs) have been shown to play a key role in the initiation and maintenance of immune responses to microbial pathogens as well as to allergens, but the exact mechanisms of their involvement in allergic responses and Th2 cell differentiation have remained elusive. Using retagging, we i...
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American Society for Biochemistry and Molecular Biology
2012
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| Online Access: | https://eprints.nottingham.ac.uk/2297/ |
| _version_ | 1848790749239312384 |
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| author | Emara, Mohamed Royer, Pierre-Joseph Mahdavi, Jafar Shakib, Farouk Ghaemmaghami, Amir M. |
| author_facet | Emara, Mohamed Royer, Pierre-Joseph Mahdavi, Jafar Shakib, Farouk Ghaemmaghami, Amir M. |
| author_sort | Emara, Mohamed |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Dendritic cells (DCs) have been shown to play a key role in the initiation and maintenance of immune responses to microbial pathogens as well as to allergens, but the exact mechanisms of their involvement in allergic responses and Th2 cell differentiation have remained elusive. Using retagging, we identified DCSIGN as a novel receptor involved in the initial recognition and uptake of the major house dust mite and dog allergens Der p 1 and Can f 1, respectively. To confirm this, we used gene silencing
to specifically inhibit DC-SIGN expression by DCs followed by allergen uptake studies. Binding and uptake of Der p 1 and Can f 1 allergens was assessed by ELISA and flow cytometry. Intriguingly, our data showed that silencing DC-SIGN on DCs promotes a Th2 phenotype in DC/T cell co-cultures. These findings should lead to better understanding of the molecular basis of allergen-induced Th2 cell polarization and in doing so paves the way for the rational design of novel intervention strategies by targeting allergen receptors on innate immune cells or their carbohydrate counterstructures on allergens. |
| first_indexed | 2025-11-14T18:17:33Z |
| format | Article |
| id | nottingham-2297 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:17:33Z |
| publishDate | 2012 |
| publisher | American Society for Biochemistry and Molecular Biology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-22972020-05-04T16:32:30Z https://eprints.nottingham.ac.uk/2297/ Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite Emara, Mohamed Royer, Pierre-Joseph Mahdavi, Jafar Shakib, Farouk Ghaemmaghami, Amir M. Dendritic cells (DCs) have been shown to play a key role in the initiation and maintenance of immune responses to microbial pathogens as well as to allergens, but the exact mechanisms of their involvement in allergic responses and Th2 cell differentiation have remained elusive. Using retagging, we identified DCSIGN as a novel receptor involved in the initial recognition and uptake of the major house dust mite and dog allergens Der p 1 and Can f 1, respectively. To confirm this, we used gene silencing to specifically inhibit DC-SIGN expression by DCs followed by allergen uptake studies. Binding and uptake of Der p 1 and Can f 1 allergens was assessed by ELISA and flow cytometry. Intriguingly, our data showed that silencing DC-SIGN on DCs promotes a Th2 phenotype in DC/T cell co-cultures. These findings should lead to better understanding of the molecular basis of allergen-induced Th2 cell polarization and in doing so paves the way for the rational design of novel intervention strategies by targeting allergen receptors on innate immune cells or their carbohydrate counterstructures on allergens. American Society for Biochemistry and Molecular Biology 2012-02-17 Article PeerReviewed Emara, Mohamed, Royer, Pierre-Joseph, Mahdavi, Jafar, Shakib, Farouk and Ghaemmaghami, Amir M. (2012) Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite. Journal of Biological Chemistry, 287 (8). pp. 5756-5763. ISSN 0021-9258 Immunology Glycobiology Extracellular Matrices http://www.jbc.org/content/287/8/5756 doi:10.1074/jbc.M111.312520 doi:10.1074/jbc.M111.312520 |
| spellingShingle | Immunology Glycobiology Extracellular Matrices Emara, Mohamed Royer, Pierre-Joseph Mahdavi, Jafar Shakib, Farouk Ghaemmaghami, Amir M. Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite |
| title | Retagging identifies dendritic cell-specific intercellular
adhesion molecule-3 (ICAM3)-grabbing non-integrin
(DC-SIGN) protein as a novel receptor for a major allergen
from house dust mite |
| title_full | Retagging identifies dendritic cell-specific intercellular
adhesion molecule-3 (ICAM3)-grabbing non-integrin
(DC-SIGN) protein as a novel receptor for a major allergen
from house dust mite |
| title_fullStr | Retagging identifies dendritic cell-specific intercellular
adhesion molecule-3 (ICAM3)-grabbing non-integrin
(DC-SIGN) protein as a novel receptor for a major allergen
from house dust mite |
| title_full_unstemmed | Retagging identifies dendritic cell-specific intercellular
adhesion molecule-3 (ICAM3)-grabbing non-integrin
(DC-SIGN) protein as a novel receptor for a major allergen
from house dust mite |
| title_short | Retagging identifies dendritic cell-specific intercellular
adhesion molecule-3 (ICAM3)-grabbing non-integrin
(DC-SIGN) protein as a novel receptor for a major allergen
from house dust mite |
| title_sort | retagging identifies dendritic cell-specific intercellular
adhesion molecule-3 (icam3)-grabbing non-integrin
(dc-sign) protein as a novel receptor for a major allergen
from house dust mite |
| topic | Immunology Glycobiology Extracellular Matrices |
| url | https://eprints.nottingham.ac.uk/2297/ https://eprints.nottingham.ac.uk/2297/ https://eprints.nottingham.ac.uk/2297/ |