Continuous Wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16-1 heat-shock gene expression in Caenorhabditis elegans

Recent data suggest that there might be a subtle thermal explanation for the apparent induction by radiofrequency (RF) radiation of transgene expression from a small-heat-shock-protein (hsp16-1) promoter in the nematode, Caenorhabditis elegans. The RF fields used in the C. elegans study were much we...

Full description

Bibliographic Details
Main Authors: Dawe, Adam S., Nylund, Reeta, Leszczynski, Dariusz, Kuster, Niels, Reader, Tom, de Pomerai, David I.
Format: Article
Published: Wiley 2008
Online Access:https://eprints.nottingham.ac.uk/1979/
_version_ 1848790696250572800
author Dawe, Adam S.
Nylund, Reeta
Leszczynski, Dariusz
Kuster, Niels
Reader, Tom
de Pomerai, David I.
author_facet Dawe, Adam S.
Nylund, Reeta
Leszczynski, Dariusz
Kuster, Niels
Reader, Tom
de Pomerai, David I.
author_sort Dawe, Adam S.
building Nottingham Research Data Repository
collection Online Access
description Recent data suggest that there might be a subtle thermal explanation for the apparent induction by radiofrequency (RF) radiation of transgene expression from a small-heat-shock-protein (hsp16-1) promoter in the nematode, Caenorhabditis elegans. The RF fields used in the C. elegans study were much weaker (SAR 5-40 mW kg-1) than those routinely tested in many other published studies (SAR ~2 W kg-1). To resolve this disparity, we have exposed the same transgenic hsp16-1::lacZ strain of C. elegans (PC72) to higher intensity RF fields (1.8 GHz; SAR ~1.8 W kg-1). For both continuous wave (CW) and Talk-pulsed RF exposures (2.5 h at 25C), there was no indication that RF exposure could induce reporter expression above sham control levels. Thus, at much higher induced RF field strength (close to the maximum permitted exposure from a mobile telephone handset), this particular nematode heat-shock gene is not up-regulated. However, under conditions where background reporter expression was moderately elevated in the sham controls (perhaps as a result of some unknown co-stressor), we found some evidence that reporter expression may be reduced by ~15% following exposure to either Talk-pulsed or CW RF fields.
first_indexed 2025-11-14T18:16:43Z
format Article
id nottingham-1979
institution University of Nottingham Malaysia Campus
institution_category Local University
last_indexed 2025-11-14T18:16:43Z
publishDate 2008
publisher Wiley
recordtype eprints
repository_type Digital Repository
spelling nottingham-19792020-05-04T20:27:39Z https://eprints.nottingham.ac.uk/1979/ Continuous Wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16-1 heat-shock gene expression in Caenorhabditis elegans Dawe, Adam S. Nylund, Reeta Leszczynski, Dariusz Kuster, Niels Reader, Tom de Pomerai, David I. Recent data suggest that there might be a subtle thermal explanation for the apparent induction by radiofrequency (RF) radiation of transgene expression from a small-heat-shock-protein (hsp16-1) promoter in the nematode, Caenorhabditis elegans. The RF fields used in the C. elegans study were much weaker (SAR 5-40 mW kg-1) than those routinely tested in many other published studies (SAR ~2 W kg-1). To resolve this disparity, we have exposed the same transgenic hsp16-1::lacZ strain of C. elegans (PC72) to higher intensity RF fields (1.8 GHz; SAR ~1.8 W kg-1). For both continuous wave (CW) and Talk-pulsed RF exposures (2.5 h at 25C), there was no indication that RF exposure could induce reporter expression above sham control levels. Thus, at much higher induced RF field strength (close to the maximum permitted exposure from a mobile telephone handset), this particular nematode heat-shock gene is not up-regulated. However, under conditions where background reporter expression was moderately elevated in the sham controls (perhaps as a result of some unknown co-stressor), we found some evidence that reporter expression may be reduced by ~15% following exposure to either Talk-pulsed or CW RF fields. Wiley 2008 Article PeerReviewed Dawe, Adam S., Nylund, Reeta, Leszczynski, Dariusz, Kuster, Niels, Reader, Tom and de Pomerai, David I. (2008) Continuous Wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16-1 heat-shock gene expression in Caenorhabditis elegans. Bioelectromagnetics, 29 (2). pp. 92-99. ISSN 1521-186X http://onlinelibrary.wiley.com/doi/10.1002/bem.20366/abstract doi:10.1002/bem.20366 doi:10.1002/bem.20366
spellingShingle Dawe, Adam S.
Nylund, Reeta
Leszczynski, Dariusz
Kuster, Niels
Reader, Tom
de Pomerai, David I.
Continuous Wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16-1 heat-shock gene expression in Caenorhabditis elegans
title Continuous Wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16-1 heat-shock gene expression in Caenorhabditis elegans
title_full Continuous Wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16-1 heat-shock gene expression in Caenorhabditis elegans
title_fullStr Continuous Wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16-1 heat-shock gene expression in Caenorhabditis elegans
title_full_unstemmed Continuous Wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16-1 heat-shock gene expression in Caenorhabditis elegans
title_short Continuous Wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16-1 heat-shock gene expression in Caenorhabditis elegans
title_sort continuous wave and simulated gsm exposure at 1.8 w/kg and 1.8 ghz do not induce hsp16-1 heat-shock gene expression in caenorhabditis elegans
url https://eprints.nottingham.ac.uk/1979/
https://eprints.nottingham.ac.uk/1979/
https://eprints.nottingham.ac.uk/1979/