Scanning electron microscopy of biomaterials
A comparison of conventional high vacuum scanning electron microscopy (HVSEM), environmental SEM (ESEM) and confocal laser scanning microscopy (CLSM) in the assessment of cell-material interactions is made. The processing of cells cultured for conventional HVSEM leads to the loss of morphological fe...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Book Section |
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Institute of Physics Publishing
2004
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| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/1443/ |
| _version_ | 1848790606911897600 |
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| author | McKinlay, K.J. Scotchford, C.A. Grant, D.M. Oliver, J.M. King, John R. Brown, Paul D. |
| author2 | McVitie, Stephen |
| author_facet | McVitie, Stephen McKinlay, K.J. Scotchford, C.A. Grant, D.M. Oliver, J.M. King, John R. Brown, Paul D. |
| author_sort | McKinlay, K.J. |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | A comparison of conventional high vacuum scanning electron microscopy (HVSEM), environmental SEM (ESEM) and confocal laser scanning microscopy (CLSM) in the assessment of cell-material interactions is made. The processing of cells cultured for conventional HVSEM leads to the loss of morphological features that are retained when using ESEM. The use of ESEM in conjunction with CLSM of the labeled cytoskeleton gives an indication of changes to the cell morphology as a consequence of incubation time and substrate surface features. |
| first_indexed | 2025-11-14T18:15:18Z |
| format | Book Section |
| id | nottingham-1443 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:15:18Z |
| publishDate | 2004 |
| publisher | Institute of Physics Publishing |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-14432020-05-04T20:31:38Z https://eprints.nottingham.ac.uk/1443/ Scanning electron microscopy of biomaterials McKinlay, K.J. Scotchford, C.A. Grant, D.M. Oliver, J.M. King, John R. Brown, Paul D. A comparison of conventional high vacuum scanning electron microscopy (HVSEM), environmental SEM (ESEM) and confocal laser scanning microscopy (CLSM) in the assessment of cell-material interactions is made. The processing of cells cultured for conventional HVSEM leads to the loss of morphological features that are retained when using ESEM. The use of ESEM in conjunction with CLSM of the labeled cytoskeleton gives an indication of changes to the cell morphology as a consequence of incubation time and substrate surface features. Institute of Physics Publishing McVitie, Stephen McComb, David 2004 Book Section PeerReviewed McKinlay, K.J., Scotchford, C.A., Grant, D.M., Oliver, J.M., King, John R. and Brown, Paul D. (2004) Scanning electron microscopy of biomaterials. In: Electron Microscopy and Analysis 2003: Proceedings of the Institute of Physics Electron Microscopy and Analysis Group Conference, University of Oxford, 3-5 September 2003. Institute of Physics conference series (179). Institute of Physics Publishing, Bristol, pp. 87-90. ISBN 0750309679 SEM ESEM CLSM Biomaterials |
| spellingShingle | SEM ESEM CLSM Biomaterials McKinlay, K.J. Scotchford, C.A. Grant, D.M. Oliver, J.M. King, John R. Brown, Paul D. Scanning electron microscopy of biomaterials |
| title | Scanning electron microscopy of biomaterials |
| title_full | Scanning electron microscopy of biomaterials |
| title_fullStr | Scanning electron microscopy of biomaterials |
| title_full_unstemmed | Scanning electron microscopy of biomaterials |
| title_short | Scanning electron microscopy of biomaterials |
| title_sort | scanning electron microscopy of biomaterials |
| topic | SEM ESEM CLSM Biomaterials |
| url | https://eprints.nottingham.ac.uk/1443/ |