Scanning electron microscopy of biomaterials

Scanning electron microscopy of biomaterials

A comparison of conventional high vacuum scanning electron microscopy (HVSEM), environmental SEM (ESEM) and confocal laser scanning microscopy (CLSM) in the assessment of cell-material interactions is made. The processing of cells cultured for conventional HVSEM leads to the loss of morphological fe...

Full description

Bibliographic Details
Main Authors: McKinlay, K.J., Scotchford, C.A., Grant, D.M., Oliver, J.M., King, John R., Brown, Paul D.
Other Authors: McVitie, Stephen
Format: Book Section
Published: Institute of Physics Publishing 2004
Subjects:
SEM ESEM CLSM Biomaterials
Online Access:https://eprints.nottingham.ac.uk/1443/
Description
Summary:A comparison of conventional high vacuum scanning electron microscopy (HVSEM), environmental SEM (ESEM) and confocal laser scanning microscopy (CLSM) in the assessment of cell-material interactions is made. The processing of cells cultured for conventional HVSEM leads to the loss of morphological features that are retained when using ESEM. The use of ESEM in conjunction with CLSM of the labeled cytoskeleton gives an indication of changes to the cell morphology as a consequence of incubation time and substrate surface features.

Similar Items