Label-free molecular imaging and discrimination of stem cells by Raman micro-spectroscopy
This thesis is focused on the development of Raman micro-spectroscopy for label free imaging and discrimination of stem cells. The thesis is divided into six chapters. Chapter 1 gives an overview of the existing techniques used for molecular analysis of cells, with emphasis on methods that allow non...
| Main Author: | |
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| Format: | Thesis (University of Nottingham only) |
| Language: | English |
| Published: |
2014
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| Online Access: | https://eprints.nottingham.ac.uk/14021/ |
| _version_ | 1848791862764109824 |
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| author | Ghita, Adrian |
| author_facet | Ghita, Adrian |
| author_sort | Ghita, Adrian |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | This thesis is focused on the development of Raman micro-spectroscopy for label free imaging and discrimination of stem cells. The thesis is divided into six chapters. Chapter 1 gives an overview of the existing techniques used for molecular analysis of cells, with emphasis on methods that allow non-invasive label-free imaging. A literature review of the main relevant applications of Raman micro-spectroscopy for imaging cells was also included. Chapter 2 discusses the basic theoretical principles of Raman scattering and design of Raman micro-spectrometers. The practical aspects related to the design of an optimised Raman micro-spectrometer are presented in Chapter 3 along with experimental characterisation of its performance. The chapter concludes with examples of Raman spectral maps of endothelial cells. Chapter 4 and Chapter 5 present experimental results obtained by Raman micro-spectroscopy for molecular analysis of live neural and mesenchymal stem cells. In these investigations Raman spectroscopy was used to identify, image and quantify spectral markers for label-free discrimination between glial cells and their neural progeny. The potential of Raman micro-spectroscopy to measure timecourse molecular changes of individual bone nodules was demonstrated in Chapter 5. Future work and final conclusion are discussed in Chapter six of this thesis. |
| first_indexed | 2025-11-14T18:35:15Z |
| format | Thesis (University of Nottingham only) |
| id | nottingham-14021 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T18:35:15Z |
| publishDate | 2014 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-140212025-02-28T11:28:26Z https://eprints.nottingham.ac.uk/14021/ Label-free molecular imaging and discrimination of stem cells by Raman micro-spectroscopy Ghita, Adrian This thesis is focused on the development of Raman micro-spectroscopy for label free imaging and discrimination of stem cells. The thesis is divided into six chapters. Chapter 1 gives an overview of the existing techniques used for molecular analysis of cells, with emphasis on methods that allow non-invasive label-free imaging. A literature review of the main relevant applications of Raman micro-spectroscopy for imaging cells was also included. Chapter 2 discusses the basic theoretical principles of Raman scattering and design of Raman micro-spectrometers. The practical aspects related to the design of an optimised Raman micro-spectrometer are presented in Chapter 3 along with experimental characterisation of its performance. The chapter concludes with examples of Raman spectral maps of endothelial cells. Chapter 4 and Chapter 5 present experimental results obtained by Raman micro-spectroscopy for molecular analysis of live neural and mesenchymal stem cells. In these investigations Raman spectroscopy was used to identify, image and quantify spectral markers for label-free discrimination between glial cells and their neural progeny. The potential of Raman micro-spectroscopy to measure timecourse molecular changes of individual bone nodules was demonstrated in Chapter 5. Future work and final conclusion are discussed in Chapter six of this thesis. 2014-07-10 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en arr https://eprints.nottingham.ac.uk/14021/1/PhD_thesis_Adrian_Ghita.pdf Ghita, Adrian (2014) Label-free molecular imaging and discrimination of stem cells by Raman micro-spectroscopy. PhD thesis, University of Nottingham. |
| spellingShingle | Ghita, Adrian Label-free molecular imaging and discrimination of stem cells by Raman micro-spectroscopy |
| title | Label-free molecular imaging and discrimination of stem cells by Raman micro-spectroscopy |
| title_full | Label-free molecular imaging and discrimination of stem cells by Raman micro-spectroscopy |
| title_fullStr | Label-free molecular imaging and discrimination of stem cells by Raman micro-spectroscopy |
| title_full_unstemmed | Label-free molecular imaging and discrimination of stem cells by Raman micro-spectroscopy |
| title_short | Label-free molecular imaging and discrimination of stem cells by Raman micro-spectroscopy |
| title_sort | label-free molecular imaging and discrimination of stem cells by raman micro-spectroscopy |
| url | https://eprints.nottingham.ac.uk/14021/ |