An investigation of the regulation and physiological role of Listeria monocytogenes extracellular polymer
It was shown that Listeria monocytogenes cells grown in a defined minimal, MCDB202, showed enhanced extracellular polymeric substances production compared to BHI. On the other hand, it was reported that in L. monocytogenes luxS mutant, AI-2 reduction and biofilm enhancement were seen. It is hypothes...
| Main Author: | |
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| Format: | Thesis (University of Nottingham only) |
| Language: | English |
| Published: |
2013
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| Online Access: | https://eprints.nottingham.ac.uk/12948/ |
| _version_ | 1848791614863966208 |
|---|---|
| author | Wong, Ho Ting Lawrence |
| author_facet | Wong, Ho Ting Lawrence |
| author_sort | Wong, Ho Ting Lawrence |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | It was shown that Listeria monocytogenes cells grown in a defined minimal, MCDB202, showed enhanced extracellular polymeric substances production compared to BHI. On the other hand, it was reported that in L. monocytogenes luxS mutant, AI-2 reduction and biofilm enhancement were seen. It is hypotheses that there could be a linkage between the AI-2 signaling system and the EPS formation. The expression of EPS could be induced by the reduction in AI-2.
The main aim of the research is to study this EPS formation in minimal media, how is it linked to AI-2 production, the function of the EPS as well as to figure out the linkage between EPS formation with cap genes found in Listeria genome.
It was shown that MCDB202 have caused an increase in surface hydrophobicity of the cells. However, cells grown in the defined media did not induced better attachment and biofilm formation towards hydrophobic surfaces. And cells grown in MCDB202 were shown less capable to infect eukaryotic cells in the cell invasion assay. On the other hand, AI-2 production was shown to be relative lower in Listeria cell grown in minimal media MCDB202) than rich media (BHI). Bioinformatics study has shown that only capA homologues, but no capBCDE homologues, were found in Listeria genome. However, the bioinformatics works have shown that the capA homologues are unlikely to be contributing the EPS seen produced in Listeria monocytogenes. This was further supported in the expression assay that the two genes were not highly expressed in MCDB media. |
| first_indexed | 2025-11-14T18:31:19Z |
| format | Thesis (University of Nottingham only) |
| id | nottingham-12948 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T18:31:19Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-129482025-02-28T11:22:17Z https://eprints.nottingham.ac.uk/12948/ An investigation of the regulation and physiological role of Listeria monocytogenes extracellular polymer Wong, Ho Ting Lawrence It was shown that Listeria monocytogenes cells grown in a defined minimal, MCDB202, showed enhanced extracellular polymeric substances production compared to BHI. On the other hand, it was reported that in L. monocytogenes luxS mutant, AI-2 reduction and biofilm enhancement were seen. It is hypotheses that there could be a linkage between the AI-2 signaling system and the EPS formation. The expression of EPS could be induced by the reduction in AI-2. The main aim of the research is to study this EPS formation in minimal media, how is it linked to AI-2 production, the function of the EPS as well as to figure out the linkage between EPS formation with cap genes found in Listeria genome. It was shown that MCDB202 have caused an increase in surface hydrophobicity of the cells. However, cells grown in the defined media did not induced better attachment and biofilm formation towards hydrophobic surfaces. And cells grown in MCDB202 were shown less capable to infect eukaryotic cells in the cell invasion assay. On the other hand, AI-2 production was shown to be relative lower in Listeria cell grown in minimal media MCDB202) than rich media (BHI). Bioinformatics study has shown that only capA homologues, but no capBCDE homologues, were found in Listeria genome. However, the bioinformatics works have shown that the capA homologues are unlikely to be contributing the EPS seen produced in Listeria monocytogenes. This was further supported in the expression assay that the two genes were not highly expressed in MCDB media. 2013-03-15 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en arr https://eprints.nottingham.ac.uk/12948/1/Lawrence_Wong_Thesis.pdf Wong, Ho Ting Lawrence (2013) An investigation of the regulation and physiological role of Listeria monocytogenes extracellular polymer. PhD thesis, University of Nottingham. |
| spellingShingle | Wong, Ho Ting Lawrence An investigation of the regulation and physiological role of Listeria monocytogenes extracellular polymer |
| title | An investigation of the regulation and physiological role of Listeria monocytogenes extracellular polymer |
| title_full | An investigation of the regulation and physiological role of Listeria monocytogenes extracellular polymer |
| title_fullStr | An investigation of the regulation and physiological role of Listeria monocytogenes extracellular polymer |
| title_full_unstemmed | An investigation of the regulation and physiological role of Listeria monocytogenes extracellular polymer |
| title_short | An investigation of the regulation and physiological role of Listeria monocytogenes extracellular polymer |
| title_sort | investigation of the regulation and physiological role of listeria monocytogenes extracellular polymer |
| url | https://eprints.nottingham.ac.uk/12948/ |