Increasing the water use efficiency (WUE) of tomato (S. lycopersicum) via manipulation of the abscisic acid (ABA) biosynthesis pathway
Breeding plants that produce equivalent growth with reduced water input (improved water use efficiency (WUE)) is necessary for sustained future crop production. Water deficit induces redistribution and synthesis of the phytohormone abscisic acid (ABA) thereby restricting transpiration. ABA is synthe...
| Main Author: | |
|---|---|
| Format: | Thesis (University of Nottingham only) |
| Language: | English |
| Published: |
2011
|
| Subjects: | |
| Online Access: | https://eprints.nottingham.ac.uk/11948/ |
| _version_ | 1848791396115283968 |
|---|---|
| author | White, Charlotte Anne |
| author_facet | White, Charlotte Anne |
| author_sort | White, Charlotte Anne |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | Breeding plants that produce equivalent growth with reduced water input (improved water use efficiency (WUE)) is necessary for sustained future crop production. Water deficit induces redistribution and synthesis of the phytohormone abscisic acid (ABA) thereby restricting transpiration. ABA is synthesised via cleavage of oxygenated carotenoids (xanthophylls). This work involved two approaches and three key ratelimiting enzymes in the ABA biosynthesis pathway: 9-cis-epoxycarotenoid dioxygenase (NCED) β-carotene hydroxylase (BCH); and phytoene synthase (PSY). Increasing ABA production under optimal conditions by overexpressing SlNCED1 has been shown to reduce stomatal conductance and improve WUE. The first approach, part of a larger programme exploring the allelic variation of NCED1 in wild tomato species, describes the introgression of S. galapagense and S. neorickii NCED1 alleles into the cultivated tomato background S. lycopersicum cv. Ailsa Craig. Plants homozygous for SgNCED1, SnNCED1 and SlNCED1 alleles were compared for water use gravimetrically. It was concluded that SgNCED1 and SnNCED1 did not improve WUE.
The second approach involved creating a transgenic rootstock that might provide sufficient extra root-sourced ABA under non-stressed conditions to improve WUE of a non-transgenic scion. Root tissues contain less carotenoids than photosynthetic tissues, which may limit increases in root ABA biosynthesis. To increase precursor flux through the pathway, transgenic lines simultaneously over-expressing SlPSY1, SlBCH2, and SlNCED1 were created by crossing two double transgenic lines. Isolated roots of a selected triple line (H-22-8-8) accumulated increased concentrations of carotenoids, ABA and ABA catabolites. Gravimetric water use trials revealed that WT/H-22-8-8 (scion/rootstock) grafts frequently showed improved; TEp, δ13C, exudate flow rate and ABA concentration. H-22-8-8 rootstock conferred a consistent, modest, daily water saving; significant in the absence of water stress. This indicates that the triple rootstock is capable of restricting stomatal opening and water use, without reducing biomass production. |
| first_indexed | 2025-11-14T18:27:50Z |
| format | Thesis (University of Nottingham only) |
| id | nottingham-11948 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T18:27:50Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-119482025-02-28T11:16:40Z https://eprints.nottingham.ac.uk/11948/ Increasing the water use efficiency (WUE) of tomato (S. lycopersicum) via manipulation of the abscisic acid (ABA) biosynthesis pathway White, Charlotte Anne Breeding plants that produce equivalent growth with reduced water input (improved water use efficiency (WUE)) is necessary for sustained future crop production. Water deficit induces redistribution and synthesis of the phytohormone abscisic acid (ABA) thereby restricting transpiration. ABA is synthesised via cleavage of oxygenated carotenoids (xanthophylls). This work involved two approaches and three key ratelimiting enzymes in the ABA biosynthesis pathway: 9-cis-epoxycarotenoid dioxygenase (NCED) β-carotene hydroxylase (BCH); and phytoene synthase (PSY). Increasing ABA production under optimal conditions by overexpressing SlNCED1 has been shown to reduce stomatal conductance and improve WUE. The first approach, part of a larger programme exploring the allelic variation of NCED1 in wild tomato species, describes the introgression of S. galapagense and S. neorickii NCED1 alleles into the cultivated tomato background S. lycopersicum cv. Ailsa Craig. Plants homozygous for SgNCED1, SnNCED1 and SlNCED1 alleles were compared for water use gravimetrically. It was concluded that SgNCED1 and SnNCED1 did not improve WUE. The second approach involved creating a transgenic rootstock that might provide sufficient extra root-sourced ABA under non-stressed conditions to improve WUE of a non-transgenic scion. Root tissues contain less carotenoids than photosynthetic tissues, which may limit increases in root ABA biosynthesis. To increase precursor flux through the pathway, transgenic lines simultaneously over-expressing SlPSY1, SlBCH2, and SlNCED1 were created by crossing two double transgenic lines. Isolated roots of a selected triple line (H-22-8-8) accumulated increased concentrations of carotenoids, ABA and ABA catabolites. Gravimetric water use trials revealed that WT/H-22-8-8 (scion/rootstock) grafts frequently showed improved; TEp, δ13C, exudate flow rate and ABA concentration. H-22-8-8 rootstock conferred a consistent, modest, daily water saving; significant in the absence of water stress. This indicates that the triple rootstock is capable of restricting stomatal opening and water use, without reducing biomass production. 2011-07-14 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en arr https://eprints.nottingham.ac.uk/11948/1/C_A_WhiteThesis_Final_Corrected.pdf White, Charlotte Anne (2011) Increasing the water use efficiency (WUE) of tomato (S. lycopersicum) via manipulation of the abscisic acid (ABA) biosynthesis pathway. PhD thesis, University of Nottingham. water use efficiency WUE abscisic acid ABA water management |
| spellingShingle | water use efficiency WUE abscisic acid ABA water management White, Charlotte Anne Increasing the water use efficiency (WUE) of tomato (S. lycopersicum) via manipulation of the abscisic acid (ABA) biosynthesis pathway |
| title | Increasing the water use efficiency (WUE) of tomato
(S. lycopersicum) via manipulation of the abscisic acid
(ABA) biosynthesis pathway |
| title_full | Increasing the water use efficiency (WUE) of tomato
(S. lycopersicum) via manipulation of the abscisic acid
(ABA) biosynthesis pathway |
| title_fullStr | Increasing the water use efficiency (WUE) of tomato
(S. lycopersicum) via manipulation of the abscisic acid
(ABA) biosynthesis pathway |
| title_full_unstemmed | Increasing the water use efficiency (WUE) of tomato
(S. lycopersicum) via manipulation of the abscisic acid
(ABA) biosynthesis pathway |
| title_short | Increasing the water use efficiency (WUE) of tomato
(S. lycopersicum) via manipulation of the abscisic acid
(ABA) biosynthesis pathway |
| title_sort | increasing the water use efficiency (wue) of tomato
(s. lycopersicum) via manipulation of the abscisic acid
(aba) biosynthesis pathway |
| topic | water use efficiency WUE abscisic acid ABA water management |
| url | https://eprints.nottingham.ac.uk/11948/ |