Towards single photon detection with a practical EMCCD
The stochastic transfer function (STF) is a metric for imaging of a microscope that includes the idea of noise, this project attempted to obtain the STF by Monte Carlo method with photon counting device. In this project, a very sensitive Electron-multiplying CCD (EMCCD) camera is evaluated for its p...
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| Format: | Thesis (University of Nottingham only) |
| Language: | English |
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2010
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| Online Access: | https://eprints.nottingham.ac.uk/11251/ |
| _version_ | 1848791230840832000 |
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| author | Chen, Jingsi |
| author_facet | Chen, Jingsi |
| author_sort | Chen, Jingsi |
| building | Nottingham Research Data Repository |
| collection | Online Access |
| description | The stochastic transfer function (STF) is a metric for imaging of a microscope that includes the idea of noise, this project attempted to obtain the STF by Monte Carlo method with photon counting device. In this project, a very sensitive Electron-multiplying CCD (EMCCD) camera is evaluated for its photon counting performance on STF measurement. The evaluation system employs a beam with a very weak intensity laser to illuminate the EMCCD, the output signal and background noise of the EMCCD is recorded and statistical data is obtained. The EMCCD output signal distribution under different conditions is given by a simulation. Moreover, photon counting in TIRF with fluorescent beads is also carried out to simulate the practical STF measurement condition. Although the experiment results suggest that the EMCCD camera used in this project is not capable of performing photon counting for the Monte Carlo method, the performance of this EMCCD camera is studied in detail. The evaluation method and data can be used to evaluate other EMCCD or photon counting devices. |
| first_indexed | 2025-11-14T18:25:13Z |
| format | Thesis (University of Nottingham only) |
| id | nottingham-11251 |
| institution | University of Nottingham Malaysia Campus |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T18:25:13Z |
| publishDate | 2010 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | nottingham-112512025-02-28T11:12:16Z https://eprints.nottingham.ac.uk/11251/ Towards single photon detection with a practical EMCCD Chen, Jingsi The stochastic transfer function (STF) is a metric for imaging of a microscope that includes the idea of noise, this project attempted to obtain the STF by Monte Carlo method with photon counting device. In this project, a very sensitive Electron-multiplying CCD (EMCCD) camera is evaluated for its photon counting performance on STF measurement. The evaluation system employs a beam with a very weak intensity laser to illuminate the EMCCD, the output signal and background noise of the EMCCD is recorded and statistical data is obtained. The EMCCD output signal distribution under different conditions is given by a simulation. Moreover, photon counting in TIRF with fluorescent beads is also carried out to simulate the practical STF measurement condition. Although the experiment results suggest that the EMCCD camera used in this project is not capable of performing photon counting for the Monte Carlo method, the performance of this EMCCD camera is studied in detail. The evaluation method and data can be used to evaluate other EMCCD or photon counting devices. 2010-07-21 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en arr https://eprints.nottingham.ac.uk/11251/1/Jingsi_Chen_Final_thesis.pdf Chen, Jingsi (2010) Towards single photon detection with a practical EMCCD. MSc(Res) thesis, University of Nottingham. Stochastic transfer function Photon counting device EMCCD camera Photon detectors Charge coupled devices |
| spellingShingle | Stochastic transfer function Photon counting device EMCCD camera Photon detectors Charge coupled devices Chen, Jingsi Towards single photon detection with a practical EMCCD |
| title | Towards single photon detection with a practical EMCCD |
| title_full | Towards single photon detection with a practical EMCCD |
| title_fullStr | Towards single photon detection with a practical EMCCD |
| title_full_unstemmed | Towards single photon detection with a practical EMCCD |
| title_short | Towards single photon detection with a practical EMCCD |
| title_sort | towards single photon detection with a practical emccd |
| topic | Stochastic transfer function Photon counting device EMCCD camera Photon detectors Charge coupled devices |
| url | https://eprints.nottingham.ac.uk/11251/ |