Nitrogen-phosphorus relationships in lichens

Nitrogen enrichment promotes phosphomonoesterase (PME) activity in the common heathland lichen Cladonia portentosa. This is associated with a marked increase in thallus N:P mass ratio and significant up-regulation of inorganic phosphate uptake capacity, evidencing a shift from N-limited to P-limite...

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Main Author: Hogan, Erika
Format: Thesis (University of Nottingham only)
Language:English
Published: 2009
Subjects:
Online Access:https://eprints.nottingham.ac.uk/10906/
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author Hogan, Erika
author_facet Hogan, Erika
author_sort Hogan, Erika
building Nottingham Research Data Repository
collection Online Access
description Nitrogen enrichment promotes phosphomonoesterase (PME) activity in the common heathland lichen Cladonia portentosa. This is associated with a marked increase in thallus N:P mass ratio and significant up-regulation of inorganic phosphate uptake capacity, evidencing a shift from N-limited to P-limited growth. Phosphomonoesterase activity in C. portentosa responds rapidly to change in N deposition load, with a significant increase in activity recorded within 6 months of transplantation from a low-N to high-N site. The location of PME activity in C. portentosa was revealed using a fluorescent marker and was found to be concentrated on both the outer and inner surfaces of the hollow ‘tube-like’ thallus branches. Activity appeared to be associated exclusively with the mycobiont and was located within the hyphal-lumina, consistent with a membrane bound ecto-enzyme. High PME activity in axenic mycobiont cultures of C. portentosa provided further evidence of a fungal location and confirmed that rates of activity in this lichen are amongst the highest reported for any other plant/fungal system in the literature. Different classes of phosphatases were assayed in a range of N2-fixing and non-N2-fixing lichens in an oligotrophic subarctic environment. No relationship was found between the capacities for nitrogenase and PME activities. Maximum rates of PME activity were recorded in fruticose mat-forming lichens which capture nutrients predominantly from atmospheric deposits. 5´ nucleotide phosphodiesterase activity was readily detected in N2-fixing lichens and was particularly high in rhizine-rich regions of foliose terricolous lichen thalli, consistent with the utilisation of organic phosphates from soil and litter sources.
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format Thesis (University of Nottingham only)
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language English
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publishDate 2009
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spelling nottingham-109062025-02-28T11:10:12Z https://eprints.nottingham.ac.uk/10906/ Nitrogen-phosphorus relationships in lichens Hogan, Erika Nitrogen enrichment promotes phosphomonoesterase (PME) activity in the common heathland lichen Cladonia portentosa. This is associated with a marked increase in thallus N:P mass ratio and significant up-regulation of inorganic phosphate uptake capacity, evidencing a shift from N-limited to P-limited growth. Phosphomonoesterase activity in C. portentosa responds rapidly to change in N deposition load, with a significant increase in activity recorded within 6 months of transplantation from a low-N to high-N site. The location of PME activity in C. portentosa was revealed using a fluorescent marker and was found to be concentrated on both the outer and inner surfaces of the hollow ‘tube-like’ thallus branches. Activity appeared to be associated exclusively with the mycobiont and was located within the hyphal-lumina, consistent with a membrane bound ecto-enzyme. High PME activity in axenic mycobiont cultures of C. portentosa provided further evidence of a fungal location and confirmed that rates of activity in this lichen are amongst the highest reported for any other plant/fungal system in the literature. Different classes of phosphatases were assayed in a range of N2-fixing and non-N2-fixing lichens in an oligotrophic subarctic environment. No relationship was found between the capacities for nitrogenase and PME activities. Maximum rates of PME activity were recorded in fruticose mat-forming lichens which capture nutrients predominantly from atmospheric deposits. 5´ nucleotide phosphodiesterase activity was readily detected in N2-fixing lichens and was particularly high in rhizine-rich regions of foliose terricolous lichen thalli, consistent with the utilisation of organic phosphates from soil and litter sources. 2009-12-10 Thesis (University of Nottingham only) NonPeerReviewed application/pdf en arr https://eprints.nottingham.ac.uk/10906/1/Erika_Hogan_Thesis_250809.pdf Hogan, Erika (2009) Nitrogen-phosphorus relationships in lichens. PhD thesis, University of Nottingham. Nitrogen enrichment Phosphomonoesterase activity Nutrient acquisition in lichens Phosphatase activity Phosphorus in lichens
spellingShingle Nitrogen enrichment
Phosphomonoesterase activity
Nutrient acquisition in lichens
Phosphatase activity
Phosphorus in lichens
Hogan, Erika
Nitrogen-phosphorus relationships in lichens
title Nitrogen-phosphorus relationships in lichens
title_full Nitrogen-phosphorus relationships in lichens
title_fullStr Nitrogen-phosphorus relationships in lichens
title_full_unstemmed Nitrogen-phosphorus relationships in lichens
title_short Nitrogen-phosphorus relationships in lichens
title_sort nitrogen-phosphorus relationships in lichens
topic Nitrogen enrichment
Phosphomonoesterase activity
Nutrient acquisition in lichens
Phosphatase activity
Phosphorus in lichens
url https://eprints.nottingham.ac.uk/10906/