Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies
Oil palm (Elaeis guineensis Jacq.) is the highest yielding oil-bearing crop. However, being a perennial crop, genetic improvement of oil palm is extremely slow. Indeed, compared to other annual oil crops such as soybean and rapeseed, genetic manipulations remained less important. Therefore, to remai...
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| Format: | Article |
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Pontificia Universidad Católica de Valparaíso -- Chile
2005
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| Online Access: | http://shdl.mmu.edu.my/2243/ |
| _version_ | 1848790002757009408 |
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| author | Abdullah, Ruslan Zainal, Alizah Wee, Yew Heng Leaw, Chui Li Yeap, Chee Beng Lee, Mei Phing Abdullah Sirajuddin, Salwa Yap, Winnie Soo Ping Joseph, Juanita Lourdes Jusoh, Siti Azma |
| author_facet | Abdullah, Ruslan Zainal, Alizah Wee, Yew Heng Leaw, Chui Li Yeap, Chee Beng Lee, Mei Phing Abdullah Sirajuddin, Salwa Yap, Winnie Soo Ping Joseph, Juanita Lourdes Jusoh, Siti Azma |
| author_sort | Abdullah, Ruslan |
| building | MMU Institutional Repository |
| collection | Online Access |
| description | Oil palm (Elaeis guineensis Jacq.) is the highest yielding oil-bearing crop. However, being a perennial crop, genetic improvement of oil palm is extremely slow. Indeed, compared to other annual oil crops such as soybean and rapeseed, genetic manipulations remained less important. Therefore, to remain competitive, oil palm growers and breeders need new and novel approaches. In this report, the potential of immature embryos (IE) as a useful tool for oil palm genetic transformation studies was evaluated. It was evident that IEs were amenable to both direct and Agrobacterium-mediated gene transfer. Due to the abundant supply of IE, optimization of biolistic and Agrobacterium-mediated gene transfer into IEs were easily carried out. Transient transformation frequencies were comparable to other plant systems reported, with as high as 97.4% recorded for biolistic and 64.4% for Agrobacterium-mediated gene transfer. Like most moncots, oil palm tissues were less sensitive to kanamycin, geneticin and chloramphenicol. Instead, both hygromycin and phosphinotrycin were toxic 20 mg/l, making both suitable candidates for selecting putative transformants. IEs were also more responsive to in vitro manipulations as compared to other explants such as leaf and root tissues. Rapid in vitro response to callusing and embryogenesis or rapid and highly efficient direct germination resulted in a shorter culture period. This would minimize the production of abnormal clonal palms, which has been associated to chromosomal aberration due to prolonged time in culture. In addition, IEs also allows rapid and direct introduction of elite genes into breeding programs and in biclonal seed production. |
| first_indexed | 2025-11-14T18:05:41Z |
| format | Article |
| id | mmu-2243 |
| institution | Multimedia University |
| institution_category | Local University |
| last_indexed | 2025-11-14T18:05:41Z |
| publishDate | 2005 |
| publisher | Pontificia Universidad Católica de Valparaíso -- Chile |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | mmu-22432021-08-25T04:35:18Z http://shdl.mmu.edu.my/2243/ Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies Abdullah, Ruslan Zainal, Alizah Wee, Yew Heng Leaw, Chui Li Yeap, Chee Beng Lee, Mei Phing Abdullah Sirajuddin, Salwa Yap, Winnie Soo Ping Joseph, Juanita Lourdes Jusoh, Siti Azma QH Natural history Oil palm (Elaeis guineensis Jacq.) is the highest yielding oil-bearing crop. However, being a perennial crop, genetic improvement of oil palm is extremely slow. Indeed, compared to other annual oil crops such as soybean and rapeseed, genetic manipulations remained less important. Therefore, to remain competitive, oil palm growers and breeders need new and novel approaches. In this report, the potential of immature embryos (IE) as a useful tool for oil palm genetic transformation studies was evaluated. It was evident that IEs were amenable to both direct and Agrobacterium-mediated gene transfer. Due to the abundant supply of IE, optimization of biolistic and Agrobacterium-mediated gene transfer into IEs were easily carried out. Transient transformation frequencies were comparable to other plant systems reported, with as high as 97.4% recorded for biolistic and 64.4% for Agrobacterium-mediated gene transfer. Like most moncots, oil palm tissues were less sensitive to kanamycin, geneticin and chloramphenicol. Instead, both hygromycin and phosphinotrycin were toxic 20 mg/l, making both suitable candidates for selecting putative transformants. IEs were also more responsive to in vitro manipulations as compared to other explants such as leaf and root tissues. Rapid in vitro response to callusing and embryogenesis or rapid and highly efficient direct germination resulted in a shorter culture period. This would minimize the production of abnormal clonal palms, which has been associated to chromosomal aberration due to prolonged time in culture. In addition, IEs also allows rapid and direct introduction of elite genes into breeding programs and in biclonal seed production. Pontificia Universidad Católica de Valparaíso -- Chile 2005-04 Article NonPeerReviewed Abdullah, Ruslan and Zainal, Alizah and Wee, Yew Heng and Leaw, Chui Li and Yeap, Chee Beng and Lee, Mei Phing and Abdullah Sirajuddin, Salwa and Yap, Winnie Soo Ping and Joseph, Juanita Lourdes and Jusoh, Siti Azma (2005) Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies. Electronic Journal of Biotechnology, 8 (1). pp. 24-34. ISSN 0717-3458 https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100006 |
| spellingShingle | QH Natural history Abdullah, Ruslan Zainal, Alizah Wee, Yew Heng Leaw, Chui Li Yeap, Chee Beng Lee, Mei Phing Abdullah Sirajuddin, Salwa Yap, Winnie Soo Ping Joseph, Juanita Lourdes Jusoh, Siti Azma Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies |
| title | Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies |
| title_full | Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies |
| title_fullStr | Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies |
| title_full_unstemmed | Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies |
| title_short | Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies |
| title_sort | immature embryo: a useful tool for oil palm (elaeis guineensis jacq.) genetic transformation studies |
| topic | QH Natural history |
| url | http://shdl.mmu.edu.my/2243/ http://shdl.mmu.edu.my/2243/ |