Isolation and Molecular Phylogenetic Characterization of Cadmium-Tolerant Hydrocarbon Degrading Bacteria
Three bacteria isolates (A, B and C) previously shown to biodegrade hydrocarbons were subjected to cadmium (Cd) toxicity test at 0.1 mg/L and 1.0 mg/L. of the three isolates, only two (isolates A and C) were observed to survive Cd at the two tested concentration. Increasing the Cd concentration 10 t...
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| Format: | Thesis |
| Language: | English |
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2016
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| Online Access: | http://eprints.intimal.edu.my/940/ http://eprints.intimal.edu.my/940/1/103.pdf |
| _version_ | 1848766605748600832 |
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| author | Chew, Efendy |
| author_facet | Chew, Efendy |
| author_sort | Chew, Efendy |
| building | INTI Institutional Repository |
| collection | Online Access |
| description | Three bacteria isolates (A, B and C) previously shown to biodegrade hydrocarbons were subjected to cadmium (Cd) toxicity test at 0.1 mg/L and 1.0 mg/L. of the three isolates, only two (isolates A and C) were observed to survive Cd at the two tested concentration. Increasing the Cd concentration 10 times higher from 0.1 mg/L to 1.0 mg/L did not inhibit the growth of both isolates (p>0.05). However isolate C showed the highest growth (p<0.05) at the highest concentration of Cd tested at 1.0mg/L, which is 100 times higher than the permissible limit for Cd in industrial effluent according to the Malaysian Environmental Quality Regulations. Gram staining results showed that isolate C to be Gram negative. DNA was successfully extracted from isolate C, visualized as a single band on 1.2% agarose gel. Amplification of the DNA extracted using primers targeting the 16s rDNA region yielded a band size of 1500bp. The PCR product was sequenced and the sequence obtained was used successfully to construct a phylogenetic tree, identifying the Cd resistant bacteria to be Pseudomonas aeruginosa. |
| first_indexed | 2025-11-14T11:53:48Z |
| format | Thesis |
| id | intimal-940 |
| institution | INTI International University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:53:48Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | intimal-9402017-10-17T03:12:43Z http://eprints.intimal.edu.my/940/ Isolation and Molecular Phylogenetic Characterization of Cadmium-Tolerant Hydrocarbon Degrading Bacteria Chew, Efendy TD Environmental technology. Sanitary engineering Three bacteria isolates (A, B and C) previously shown to biodegrade hydrocarbons were subjected to cadmium (Cd) toxicity test at 0.1 mg/L and 1.0 mg/L. of the three isolates, only two (isolates A and C) were observed to survive Cd at the two tested concentration. Increasing the Cd concentration 10 times higher from 0.1 mg/L to 1.0 mg/L did not inhibit the growth of both isolates (p>0.05). However isolate C showed the highest growth (p<0.05) at the highest concentration of Cd tested at 1.0mg/L, which is 100 times higher than the permissible limit for Cd in industrial effluent according to the Malaysian Environmental Quality Regulations. Gram staining results showed that isolate C to be Gram negative. DNA was successfully extracted from isolate C, visualized as a single band on 1.2% agarose gel. Amplification of the DNA extracted using primers targeting the 16s rDNA region yielded a band size of 1500bp. The PCR product was sequenced and the sequence obtained was used successfully to construct a phylogenetic tree, identifying the Cd resistant bacteria to be Pseudomonas aeruginosa. 2016 Thesis NonPeerReviewed text en http://eprints.intimal.edu.my/940/1/103.pdf Chew, Efendy (2016) Isolation and Molecular Phylogenetic Characterization of Cadmium-Tolerant Hydrocarbon Degrading Bacteria. Other thesis, INTI International University. |
| spellingShingle | TD Environmental technology. Sanitary engineering Chew, Efendy Isolation and Molecular Phylogenetic Characterization of Cadmium-Tolerant Hydrocarbon Degrading Bacteria |
| title | Isolation and Molecular Phylogenetic Characterization of Cadmium-Tolerant Hydrocarbon Degrading Bacteria |
| title_full | Isolation and Molecular Phylogenetic Characterization of Cadmium-Tolerant Hydrocarbon Degrading Bacteria |
| title_fullStr | Isolation and Molecular Phylogenetic Characterization of Cadmium-Tolerant Hydrocarbon Degrading Bacteria |
| title_full_unstemmed | Isolation and Molecular Phylogenetic Characterization of Cadmium-Tolerant Hydrocarbon Degrading Bacteria |
| title_short | Isolation and Molecular Phylogenetic Characterization of Cadmium-Tolerant Hydrocarbon Degrading Bacteria |
| title_sort | isolation and molecular phylogenetic characterization of cadmium-tolerant hydrocarbon degrading bacteria |
| topic | TD Environmental technology. Sanitary engineering |
| url | http://eprints.intimal.edu.my/940/ http://eprints.intimal.edu.my/940/1/103.pdf |