Astaxanthin Production in Haematococcus pluvialis Under Lead Nanoparticle Stress in Different pH and Salinity Conditions

Haemaatococcus pluvialis is a unicellular fresh water microalga which has the highest amount of antioxidant compared to other microorganisms. It is also rich in astaxanthin, which is a carotenoid. Astaxanthin is extremely beneficial to food, pharmaceutical, nutraceutical and cosmetic industry. This...

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Main Author: Ramburuth, Shardhavi
Format: Thesis
Language:English
Published: 2016
Subjects:
Online Access:http://eprints.intimal.edu.my/894/
http://eprints.intimal.edu.my/894/1/118.pdf
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author Ramburuth, Shardhavi
author_facet Ramburuth, Shardhavi
author_sort Ramburuth, Shardhavi
building INTI Institutional Repository
collection Online Access
description Haemaatococcus pluvialis is a unicellular fresh water microalga which has the highest amount of antioxidant compared to other microorganisms. It is also rich in astaxanthin, which is a carotenoid. Astaxanthin is extremely beneficial to food, pharmaceutical, nutraceutical and cosmetic industry. This is because of its very high antioxidant activity which is very much more than vitamin C and green tea etc. Since it is so desirable by so many companies, it should be produced in large amounts. In this experiment, a new method of accumulating astaxanthin from H. puvialis is tested which is the use of lead nanoparticle in order to find the ideal condition for maximum astaxanthin production. The H. pluvialis was cultured and then subcultured in different pH and salinity conditions. The culture at the condition where there has been maximum cell growth was obtained which is pH 7 and salinity 0.25%. The optimum culture condition obtained was used to grow the H. pluvialis and the culture was subjected to lead nanoparticle stress. The astaxanthin was then extracted using the HCI-ACE method and its concentration was measured using the spectrophotometer at 480 nm. There was maximum astaxanthin production at 10ppm and therefore lead nanoparticle can be used as stimulatory for growth.
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spelling intimal-8942017-08-24T09:02:03Z http://eprints.intimal.edu.my/894/ Astaxanthin Production in Haematococcus pluvialis Under Lead Nanoparticle Stress in Different pH and Salinity Conditions Ramburuth, Shardhavi TP Chemical technology Haemaatococcus pluvialis is a unicellular fresh water microalga which has the highest amount of antioxidant compared to other microorganisms. It is also rich in astaxanthin, which is a carotenoid. Astaxanthin is extremely beneficial to food, pharmaceutical, nutraceutical and cosmetic industry. This is because of its very high antioxidant activity which is very much more than vitamin C and green tea etc. Since it is so desirable by so many companies, it should be produced in large amounts. In this experiment, a new method of accumulating astaxanthin from H. puvialis is tested which is the use of lead nanoparticle in order to find the ideal condition for maximum astaxanthin production. The H. pluvialis was cultured and then subcultured in different pH and salinity conditions. The culture at the condition where there has been maximum cell growth was obtained which is pH 7 and salinity 0.25%. The optimum culture condition obtained was used to grow the H. pluvialis and the culture was subjected to lead nanoparticle stress. The astaxanthin was then extracted using the HCI-ACE method and its concentration was measured using the spectrophotometer at 480 nm. There was maximum astaxanthin production at 10ppm and therefore lead nanoparticle can be used as stimulatory for growth. 2016 Thesis NonPeerReviewed text en http://eprints.intimal.edu.my/894/1/118.pdf Ramburuth, Shardhavi (2016) Astaxanthin Production in Haematococcus pluvialis Under Lead Nanoparticle Stress in Different pH and Salinity Conditions. Other thesis, INTI International University.
spellingShingle TP Chemical technology
Ramburuth, Shardhavi
Astaxanthin Production in Haematococcus pluvialis Under Lead Nanoparticle Stress in Different pH and Salinity Conditions
title Astaxanthin Production in Haematococcus pluvialis Under Lead Nanoparticle Stress in Different pH and Salinity Conditions
title_full Astaxanthin Production in Haematococcus pluvialis Under Lead Nanoparticle Stress in Different pH and Salinity Conditions
title_fullStr Astaxanthin Production in Haematococcus pluvialis Under Lead Nanoparticle Stress in Different pH and Salinity Conditions
title_full_unstemmed Astaxanthin Production in Haematococcus pluvialis Under Lead Nanoparticle Stress in Different pH and Salinity Conditions
title_short Astaxanthin Production in Haematococcus pluvialis Under Lead Nanoparticle Stress in Different pH and Salinity Conditions
title_sort astaxanthin production in haematococcus pluvialis under lead nanoparticle stress in different ph and salinity conditions
topic TP Chemical technology
url http://eprints.intimal.edu.my/894/
http://eprints.intimal.edu.my/894/1/118.pdf