Screening of Copper-Resisting Hydrocarbon Degrading Bacteria and Biosurfactant Production
Four bacterial isolates that were known to degrade hydrocarbons were screened for copper (Cu) resistance and biosurfactant production. Based on spectrophotometry, isolate D had the highest growth (p<0.05) followed by isolate A, isolate C and isolate B with the least growth. Biosurfactant screenin...
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| Format: | Thesis |
| Language: | English |
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2016
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| Online Access: | http://eprints.intimal.edu.my/891/ http://eprints.intimal.edu.my/891/1/115.pdf |
| _version_ | 1848766593537933312 |
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| author | Nur Nadiah, Zainal Ariff |
| author_facet | Nur Nadiah, Zainal Ariff |
| author_sort | Nur Nadiah, Zainal Ariff |
| building | INTI Institutional Repository |
| collection | Online Access |
| description | Four bacterial isolates that were known to degrade hydrocarbons were screened for copper (Cu) resistance and biosurfactant production. Based on spectrophotometry, isolate D had the highest growth (p<0.05) followed by isolate A, isolate C and isolate B with the least growth. Biosurfactant screening assays confirmed that both isolates D and A produced biosurfactant. The dry-weight of biosurfactant obtained from partial purification suggested that there was a direct relationship between the number of cells present and the amount of biosurfactant produced. Gram staining showed that isolate D and isolate A are gram negative and gram positive respectively. The results showed that gram negative bacteria was able to show tolerance towards 20.0 mg/L Cu, possible by secretion of biosurfactant to chelate Cu ions in reducing the metal toxicity. |
| first_indexed | 2025-11-14T11:53:37Z |
| format | Thesis |
| id | intimal-891 |
| institution | INTI International University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:53:37Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | intimal-8912017-08-24T08:43:17Z http://eprints.intimal.edu.my/891/ Screening of Copper-Resisting Hydrocarbon Degrading Bacteria and Biosurfactant Production Nur Nadiah, Zainal Ariff TP Chemical technology Four bacterial isolates that were known to degrade hydrocarbons were screened for copper (Cu) resistance and biosurfactant production. Based on spectrophotometry, isolate D had the highest growth (p<0.05) followed by isolate A, isolate C and isolate B with the least growth. Biosurfactant screening assays confirmed that both isolates D and A produced biosurfactant. The dry-weight of biosurfactant obtained from partial purification suggested that there was a direct relationship between the number of cells present and the amount of biosurfactant produced. Gram staining showed that isolate D and isolate A are gram negative and gram positive respectively. The results showed that gram negative bacteria was able to show tolerance towards 20.0 mg/L Cu, possible by secretion of biosurfactant to chelate Cu ions in reducing the metal toxicity. 2016 Thesis NonPeerReviewed text en http://eprints.intimal.edu.my/891/1/115.pdf Nur Nadiah, Zainal Ariff (2016) Screening of Copper-Resisting Hydrocarbon Degrading Bacteria and Biosurfactant Production. Other thesis, INTI International University. |
| spellingShingle | TP Chemical technology Nur Nadiah, Zainal Ariff Screening of Copper-Resisting Hydrocarbon Degrading Bacteria and Biosurfactant Production |
| title | Screening of Copper-Resisting Hydrocarbon Degrading Bacteria and Biosurfactant Production |
| title_full | Screening of Copper-Resisting Hydrocarbon Degrading Bacteria and Biosurfactant Production |
| title_fullStr | Screening of Copper-Resisting Hydrocarbon Degrading Bacteria and Biosurfactant Production |
| title_full_unstemmed | Screening of Copper-Resisting Hydrocarbon Degrading Bacteria and Biosurfactant Production |
| title_short | Screening of Copper-Resisting Hydrocarbon Degrading Bacteria and Biosurfactant Production |
| title_sort | screening of copper-resisting hydrocarbon degrading bacteria and biosurfactant production |
| topic | TP Chemical technology |
| url | http://eprints.intimal.edu.my/891/ http://eprints.intimal.edu.my/891/1/115.pdf |