Performance of a cyanobacteria whole cell-based fluorescence biosensor for heavy metal and pesticide detection.
Whole cell biosensors always face the challenge of low stability of biological components and short storage life. This paper reports the effects of poly(2-hydroxyethyl methacrylate) (pHEMA) immobilization on a whole cell fluorescence biosensor for the detection of heavy metals (Cu, Pb, Cd), and p...
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| Format: | Article |
| Language: | English |
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Creative Commons Attribution License (CC BY)
2013
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| Online Access: | http://eprints.intimal.edu.my/77/ http://eprints.intimal.edu.my/77/1/Performance%20of%20a%20cyanobacteria%20whole%20cell-based%20fluorescence%20biosensor%20for%20heavy%20metal%20and%20pesticide%20detection.pdf |
| _version_ | 1848766392501796864 |
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| author | Wong, Ling Shing Lee, Yook Heng Surif, Salmijah |
| author_facet | Wong, Ling Shing Lee, Yook Heng Surif, Salmijah |
| author_sort | Wong, Ling Shing |
| building | INTI Institutional Repository |
| collection | Online Access |
| description | Whole cell biosensors always face the challenge of low stability of biological
components and short storage life. This paper reports the effects of poly(2-hydroxyethyl
methacrylate) (pHEMA) immobilization on a whole cell fluorescence biosensor for the
detection of heavy metals (Cu, Pb, Cd), and pesticides (dichlorophenoxyacetic acid
(2,4-D), and chlorpyrifos). The biosensor was produced by entrapping the cyanobacterium
Anabaena torulosa on a cellulose membrane, followed by applying a layer of pHEMA, and
attaching it to a well. The well was then fixed to an optical probe which was connected to a
fluorescence spectrophotometer and an electronic reader. The optimization of the biosensor
using several factors such as amount of HEMA and drying temperature were undertaken.
The detection limits of biosensor without pHEMA for Cu, Cd, Pb, 2,4-D and chlorpyrifos
were 1.195, 0.027, 0.0100, 0.025 and 0.025 μg/L respectively. The presence of pHEMA
increased the limits of detection to 1.410, 0.250, 0.500, 0.235 and 0.117 μg/L respectively.
pHEMA is known to enhance the reproducibility of the biosensor with average relative
standard deviation (RSD) of ±1.76% for all the pollutants tested, 48% better than the
biosensor without pHEMA (RSD = ±3.73%). In storability test with Cu 5 μg/L, the
biosensor with pHEMA performed 11.5% better than the test without pHEMA on day-10
and 5.2% better on day-25. pHEMA is therefore a good candidate to be used in whole cell
biosensors as it increases reproducibility and enhances biosensor storability. |
| first_indexed | 2025-11-14T11:50:25Z |
| format | Article |
| id | intimal-77 |
| institution | INTI International University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:50:25Z |
| publishDate | 2013 |
| publisher | Creative Commons Attribution License (CC BY) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | intimal-772016-10-06T08:25:59Z http://eprints.intimal.edu.my/77/ Performance of a cyanobacteria whole cell-based fluorescence biosensor for heavy metal and pesticide detection. Wong, Ling Shing Lee, Yook Heng Surif, Salmijah TP Chemical technology Whole cell biosensors always face the challenge of low stability of biological components and short storage life. This paper reports the effects of poly(2-hydroxyethyl methacrylate) (pHEMA) immobilization on a whole cell fluorescence biosensor for the detection of heavy metals (Cu, Pb, Cd), and pesticides (dichlorophenoxyacetic acid (2,4-D), and chlorpyrifos). The biosensor was produced by entrapping the cyanobacterium Anabaena torulosa on a cellulose membrane, followed by applying a layer of pHEMA, and attaching it to a well. The well was then fixed to an optical probe which was connected to a fluorescence spectrophotometer and an electronic reader. The optimization of the biosensor using several factors such as amount of HEMA and drying temperature were undertaken. The detection limits of biosensor without pHEMA for Cu, Cd, Pb, 2,4-D and chlorpyrifos were 1.195, 0.027, 0.0100, 0.025 and 0.025 μg/L respectively. The presence of pHEMA increased the limits of detection to 1.410, 0.250, 0.500, 0.235 and 0.117 μg/L respectively. pHEMA is known to enhance the reproducibility of the biosensor with average relative standard deviation (RSD) of ±1.76% for all the pollutants tested, 48% better than the biosensor without pHEMA (RSD = ±3.73%). In storability test with Cu 5 μg/L, the biosensor with pHEMA performed 11.5% better than the test without pHEMA on day-10 and 5.2% better on day-25. pHEMA is therefore a good candidate to be used in whole cell biosensors as it increases reproducibility and enhances biosensor storability. Creative Commons Attribution License (CC BY) 2013 Article PeerReviewed text en http://eprints.intimal.edu.my/77/1/Performance%20of%20a%20cyanobacteria%20whole%20cell-based%20fluorescence%20biosensor%20for%20heavy%20metal%20and%20pesticide%20detection.pdf Wong, Ling Shing and Lee, Yook Heng and Surif, Salmijah (2013) Performance of a cyanobacteria whole cell-based fluorescence biosensor for heavy metal and pesticide detection. Sensors, 13 (5). pp. 6394-6404. ISSN 1424-8220 http://www.mdpi.com/1424-8220/13/5/6394/htm 10.3390/s130506394 |
| spellingShingle | TP Chemical technology Wong, Ling Shing Lee, Yook Heng Surif, Salmijah Performance of a cyanobacteria whole cell-based fluorescence biosensor for heavy metal and pesticide detection. |
| title | Performance of a cyanobacteria whole cell-based fluorescence biosensor for heavy metal and pesticide detection. |
| title_full | Performance of a cyanobacteria whole cell-based fluorescence biosensor for heavy metal and pesticide detection. |
| title_fullStr | Performance of a cyanobacteria whole cell-based fluorescence biosensor for heavy metal and pesticide detection. |
| title_full_unstemmed | Performance of a cyanobacteria whole cell-based fluorescence biosensor for heavy metal and pesticide detection. |
| title_short | Performance of a cyanobacteria whole cell-based fluorescence biosensor for heavy metal and pesticide detection. |
| title_sort | performance of a cyanobacteria whole cell-based fluorescence biosensor for heavy metal and pesticide detection. |
| topic | TP Chemical technology |
| url | http://eprints.intimal.edu.my/77/ http://eprints.intimal.edu.my/77/ http://eprints.intimal.edu.my/77/ http://eprints.intimal.edu.my/77/1/Performance%20of%20a%20cyanobacteria%20whole%20cell-based%20fluorescence%20biosensor%20for%20heavy%20metal%20and%20pesticide%20detection.pdf |