Nutrient Amendments of Inorganic Fertiliser and Oil Palm Empty Fruit Bunch and Their Influence on Bacterial Species Dominance and Degradation of the Associated Crude Oil Constituents

The effects of inorganic commercial fertiliser (N:P:K = 8:8:1) and oil palm empty fruit bunch (EFB) as nutrient amendments for crude oil degradation and microbial population shift by a microbial consortium [Pseudomonas sp. (UKMP-14T), Acinetobacter sp. (UKMP-12T), Trichoderma sp. (TriUKMP-1M and Tri...

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Main Authors: Ainon, Hamzah, Siti NurSyazana, Md Salleh, Wong, Kok Kee, Sarmani, Sukiman
Format: Article
Published: Taylor & Francis 2016
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Online Access:http://eprints.intimal.edu.my/733/
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author Ainon, Hamzah
Siti NurSyazana, Md Salleh
Wong, Kok Kee
Sarmani, Sukiman
author_facet Ainon, Hamzah
Siti NurSyazana, Md Salleh
Wong, Kok Kee
Sarmani, Sukiman
author_sort Ainon, Hamzah
building INTI Institutional Repository
collection Online Access
description The effects of inorganic commercial fertiliser (N:P:K = 8:8:1) and oil palm empty fruit bunch (EFB) as nutrient amendments for crude oil degradation and microbial population shift by a microbial consortium [Pseudomonas sp. (UKMP-14T), Acinetobacter sp. (UKMP-12T), Trichoderma sp. (TriUKMP-1M and TriUKMP-2M)] were assessed. The bacterial populations present during crude oil degradation were analysed by spread plate method and 16S rRNA sequences, whereas the presence of fungi was assessed by growth on potato dextrose agar. Crude oil degradation analysed using gas chromatography-flame ionisation detection showed total petroleum hydrocarbon reduced between 70 and 100%, depending on the type of amendments compared to control (≈55%) after 30 days of incubation. Nutrient amendments using NPK fertiliser or EFB were found to influence the domination of different bacterial species, which in turn preferentially utilised different hydrocarbons. This study suggested different nutrient amendments could be used to preferentially select bacteria to degrade different components of crude oil, particularly pertaining to the recalcitrant phytane. This information is very useful for application of in situ bioremediation of soil hydrocarbon contamination.
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spelling intimal-7332017-03-14T05:53:37Z http://eprints.intimal.edu.my/733/ Nutrient Amendments of Inorganic Fertiliser and Oil Palm Empty Fruit Bunch and Their Influence on Bacterial Species Dominance and Degradation of the Associated Crude Oil Constituents Ainon, Hamzah Siti NurSyazana, Md Salleh Wong, Kok Kee Sarmani, Sukiman TP Chemical technology The effects of inorganic commercial fertiliser (N:P:K = 8:8:1) and oil palm empty fruit bunch (EFB) as nutrient amendments for crude oil degradation and microbial population shift by a microbial consortium [Pseudomonas sp. (UKMP-14T), Acinetobacter sp. (UKMP-12T), Trichoderma sp. (TriUKMP-1M and TriUKMP-2M)] were assessed. The bacterial populations present during crude oil degradation were analysed by spread plate method and 16S rRNA sequences, whereas the presence of fungi was assessed by growth on potato dextrose agar. Crude oil degradation analysed using gas chromatography-flame ionisation detection showed total petroleum hydrocarbon reduced between 70 and 100%, depending on the type of amendments compared to control (≈55%) after 30 days of incubation. Nutrient amendments using NPK fertiliser or EFB were found to influence the domination of different bacterial species, which in turn preferentially utilised different hydrocarbons. This study suggested different nutrient amendments could be used to preferentially select bacteria to degrade different components of crude oil, particularly pertaining to the recalcitrant phytane. This information is very useful for application of in situ bioremediation of soil hydrocarbon contamination. Taylor & Francis 2016 Article PeerReviewed Ainon, Hamzah and Siti NurSyazana, Md Salleh and Wong, Kok Kee and Sarmani, Sukiman (2016) Nutrient Amendments of Inorganic Fertiliser and Oil Palm Empty Fruit Bunch and Their Influence on Bacterial Species Dominance and Degradation of the Associated Crude Oil Constituents. Soil and Sediment Contamination: An International Journal, 25 (3). pp. 256-265. ISSN 1532-0383 http://dx.doi.org/10.1080/15320383.2016.1124834 http://dx.doi.org/10.1080/15320383.2016.1124834
spellingShingle TP Chemical technology
Ainon, Hamzah
Siti NurSyazana, Md Salleh
Wong, Kok Kee
Sarmani, Sukiman
Nutrient Amendments of Inorganic Fertiliser and Oil Palm Empty Fruit Bunch and Their Influence on Bacterial Species Dominance and Degradation of the Associated Crude Oil Constituents
title Nutrient Amendments of Inorganic Fertiliser and Oil Palm Empty Fruit Bunch and Their Influence on Bacterial Species Dominance and Degradation of the Associated Crude Oil Constituents
title_full Nutrient Amendments of Inorganic Fertiliser and Oil Palm Empty Fruit Bunch and Their Influence on Bacterial Species Dominance and Degradation of the Associated Crude Oil Constituents
title_fullStr Nutrient Amendments of Inorganic Fertiliser and Oil Palm Empty Fruit Bunch and Their Influence on Bacterial Species Dominance and Degradation of the Associated Crude Oil Constituents
title_full_unstemmed Nutrient Amendments of Inorganic Fertiliser and Oil Palm Empty Fruit Bunch and Their Influence on Bacterial Species Dominance and Degradation of the Associated Crude Oil Constituents
title_short Nutrient Amendments of Inorganic Fertiliser and Oil Palm Empty Fruit Bunch and Their Influence on Bacterial Species Dominance and Degradation of the Associated Crude Oil Constituents
title_sort nutrient amendments of inorganic fertiliser and oil palm empty fruit bunch and their influence on bacterial species dominance and degradation of the associated crude oil constituents
topic TP Chemical technology
url http://eprints.intimal.edu.my/733/
http://eprints.intimal.edu.my/733/
http://eprints.intimal.edu.my/733/