Cryptocaryon irritans recombinant proteins as potential antigens for sero-surveillance of cryptocaryonosis
Cryptocaryonosis is a major problem for mariculture, and the absence of suitable sero-surveillance tools for the detection of cryptocaryonosis makes it difficult to screen Cryptocaryon irritans-infected fish, particularly asymptomatic fish. In this study, we proposed a serum-based assay using select...
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Wiley
2016
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| Online Access: | http://eprints.intimal.edu.my/718/ |
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| author | Lokanathan, Yogeswaran Mohd Adnan, A. Kua, Bc Nathan, Sheila |
| author_facet | Lokanathan, Yogeswaran Mohd Adnan, A. Kua, Bc Nathan, Sheila |
| author_sort | Lokanathan, Yogeswaran |
| building | INTI Institutional Repository |
| collection | Online Access |
| description | Cryptocaryonosis is a major problem for mariculture, and the absence of suitable sero-surveillance tools for the detection of cryptocaryonosis makes it difficult to screen Cryptocaryon irritans-infected fish, particularly asymptomatic fish. In this study, we proposed a serum-based assay using selected C. irritans proteins to screen infected and asymptomatic fish. Eight highly expressed genes were chosen from an earlier study on C. irritans expressed sequence tags and ciliate glutamine codons were converted to universal glutamine codons. The chemically synthesized C. irritans genes were then expressed in an Escherichia coli expression host under optimized conditions. Five C. irritans proteins were successfully expressed in E. coli and purified by affinity chromatography. These proteins were used as antigens in an enzyme-linked immunosorbent assay (ELISA) to screen sera from experimentally immunized fish and naturally infected fish. Sera from both categories of fish reacted equally well with the expressed C. irritans recombinant proteins as well as with sonicated theronts. This study demonstrated the utility of producing ciliate recombinant proteins in a heterologous expression host. An ELISA was successfully developed to diagnose infected and asymptomatic fish using the recombinant proteins as antigens. |
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| format | Article |
| id | intimal-718 |
| institution | INTI International University |
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| last_indexed | 2025-11-14T11:52:54Z |
| publishDate | 2016 |
| publisher | Wiley |
| recordtype | eprints |
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| spelling | intimal-7182017-03-13T05:46:07Z http://eprints.intimal.edu.my/718/ Cryptocaryon irritans recombinant proteins as potential antigens for sero-surveillance of cryptocaryonosis Lokanathan, Yogeswaran Mohd Adnan, A. Kua, Bc Nathan, Sheila TP Chemical technology Cryptocaryonosis is a major problem for mariculture, and the absence of suitable sero-surveillance tools for the detection of cryptocaryonosis makes it difficult to screen Cryptocaryon irritans-infected fish, particularly asymptomatic fish. In this study, we proposed a serum-based assay using selected C. irritans proteins to screen infected and asymptomatic fish. Eight highly expressed genes were chosen from an earlier study on C. irritans expressed sequence tags and ciliate glutamine codons were converted to universal glutamine codons. The chemically synthesized C. irritans genes were then expressed in an Escherichia coli expression host under optimized conditions. Five C. irritans proteins were successfully expressed in E. coli and purified by affinity chromatography. These proteins were used as antigens in an enzyme-linked immunosorbent assay (ELISA) to screen sera from experimentally immunized fish and naturally infected fish. Sera from both categories of fish reacted equally well with the expressed C. irritans recombinant proteins as well as with sonicated theronts. This study demonstrated the utility of producing ciliate recombinant proteins in a heterologous expression host. An ELISA was successfully developed to diagnose infected and asymptomatic fish using the recombinant proteins as antigens. Wiley 2016 Article PeerReviewed Lokanathan, Yogeswaran and Mohd Adnan, A. and Kua, Bc and Nathan, Sheila (2016) Cryptocaryon irritans recombinant proteins as potential antigens for sero-surveillance of cryptocaryonosis. Journal of Fish Diseases, 39 (9). pp. 1069-1083. http://onlinelibrary.wiley.com/wol1/doi/10.1111/jfd.12474/full 10.1111/jfd.12474 |
| spellingShingle | TP Chemical technology Lokanathan, Yogeswaran Mohd Adnan, A. Kua, Bc Nathan, Sheila Cryptocaryon irritans recombinant proteins as potential antigens for sero-surveillance of cryptocaryonosis |
| title | Cryptocaryon irritans recombinant proteins as potential antigens for sero-surveillance of cryptocaryonosis |
| title_full | Cryptocaryon irritans recombinant proteins as potential antigens for sero-surveillance of cryptocaryonosis |
| title_fullStr | Cryptocaryon irritans recombinant proteins as potential antigens for sero-surveillance of cryptocaryonosis |
| title_full_unstemmed | Cryptocaryon irritans recombinant proteins as potential antigens for sero-surveillance of cryptocaryonosis |
| title_short | Cryptocaryon irritans recombinant proteins as potential antigens for sero-surveillance of cryptocaryonosis |
| title_sort | cryptocaryon irritans recombinant proteins as potential antigens for sero-surveillance of cryptocaryonosis |
| topic | TP Chemical technology |
| url | http://eprints.intimal.edu.my/718/ http://eprints.intimal.edu.my/718/ http://eprints.intimal.edu.my/718/ |