Isolation of Antibiotic Resistant Bacteria from Healthy Individuals in Nilai, Negeri Sembilan.
The emergence of antibiotic resistant bacteria is a major problem worldwide. Some of the common cause for the spread of antibiotic resistant bacteria include the improper use of antibiotics and poor hygiene practices. Healthy individuals can be carriers of antibiotic resistant bacteria. One of suc...
| Main Authors: | , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://eprints.intimal.edu.my/675/ http://eprints.intimal.edu.my/675/1/Isolation%20of%20antibiotic%20resistant%20bacteria%20from%20healthy%20individuals%20in%20Nilai%2C%20Negeri%20Sembilan..pdf |
| Summary: | The emergence of antibiotic resistant bacteria is a major problem worldwide. Some of the common cause for the
spread of antibiotic resistant bacteria include the improper use of antibiotics and poor hygiene practices. Healthy individuals
can be carriers of antibiotic resistant bacteria. One of such antibiotic resistant bacteria is the methicillin resistant S. aureus
(MRSA), a common skin microflora. These individuals can transmit antibiotic resistant bacteria to others causing outbreaks
of diseases. Thus, the aim of this study was to isolate antibiotic resistant bacteria from the nasal cavity and axilla of 28
healthy individuals in Nilai, Negeri Sembilan. The pure culture of the respective isolates were grown on Mannitol Salt Agar
(MSA) and subjected to catalase test for identification. Antibiotic susceptibility testing was then conducted to determine the
susceptibility of bacteria towards the tested antibiotics using the disc diffusion method. Out of the 40 isolates, 36 isolates
showed resistance based on the BSAC guidelines, version 6.0, 2016.The bacteria that were Gram-positive cocci with clusterlike
cell arrangement, catalase positive, and resistant to oxacillin were subjected to inoculation onto Brilliance MRSA 2 agar.
Twenty-four MRSA were isolated while 12 MRSE might have been isolated. Although further studies are required, this
preliminary finding can be used to create an awareness among the public to prevent the spread of antibiotic resistant bacteria |
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