Effect of In Vitro Nitrogen Nutrition to Phalaenopsis deliciosa Seedling Growth
In a continuous effort to conserve Phalaenopsis species, the optimization of a previously designed defined medium was carried out to understand the effect of in vitro nitrogen nutrition on Phalaenopsis deliciosa Rchb.f. In order not to alter the nutrient balance, macronutrient level instead of s...
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| Format: | Article |
| Language: | English |
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Science Publications
2015
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| Online Access: | http://eprints.intimal.edu.my/640/ http://eprints.intimal.edu.my/640/1/Effect%20of%20In%20Vitro%20Nitrogen%20Nutrition%20to%20Phalaenopsis%20deliciosa.pdf |
| _version_ | 1848766527354961920 |
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| author | Choong, Chieh Wean Lim, Chin Hao Xiong, Zherui Choong, Siew Mee |
| author_facet | Choong, Chieh Wean Lim, Chin Hao Xiong, Zherui Choong, Siew Mee |
| author_sort | Choong, Chieh Wean |
| building | INTI Institutional Repository |
| collection | Online Access |
| description | In a continuous effort to conserve Phalaenopsis species, the
optimization of a previously designed defined medium was carried out to
understand the effect of in vitro nitrogen nutrition on Phalaenopsis
deliciosa Rchb.f. In order not to alter the nutrient balance, macronutrient
level instead of solely Nitrogen (N) was adjusted. Seedlings were obtained
from in vitro germination and randomly inoculated on media with various
macronutrient levels (0.3×, 0.5×, 1×, 2× and 3×). Maximum shoot yield
was observed at 2× macronutrient level. However, the best macronutrient
level, which was 1× macronutrient level, resulted in the maximum root and
seedling yield. This macronutrient level was applied on the subsequent
experiment where seedlings were inoculated on media with various nitratenitrogen
(NO3-N) to ammonium-nitrogen (NH4-N) ratios. Seedling yield
was the highest at NO3-N/NH4-N ratio of 2.0 while Root to Shoot ratio
(R/S) was the highest at NO3-N/NH4-N ratio of 5.0. NO3-N/NH4-N ratio
significantly affected Water Content (WC) of seedlings and the response to
NO3-N/NH4-N ratio was similar to R/S, correlating better root development
to higher WC. By altering NO3-N/NH4-N ratio, plant quality in terms of
yield and organ development could be encouraged. |
| first_indexed | 2025-11-14T11:52:33Z |
| format | Article |
| id | intimal-640 |
| institution | INTI International University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:52:33Z |
| publishDate | 2015 |
| publisher | Science Publications |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | intimal-6402016-12-07T08:27:39Z http://eprints.intimal.edu.my/640/ Effect of In Vitro Nitrogen Nutrition to Phalaenopsis deliciosa Seedling Growth Choong, Chieh Wean Lim, Chin Hao Xiong, Zherui Choong, Siew Mee TP Chemical technology In a continuous effort to conserve Phalaenopsis species, the optimization of a previously designed defined medium was carried out to understand the effect of in vitro nitrogen nutrition on Phalaenopsis deliciosa Rchb.f. In order not to alter the nutrient balance, macronutrient level instead of solely Nitrogen (N) was adjusted. Seedlings were obtained from in vitro germination and randomly inoculated on media with various macronutrient levels (0.3×, 0.5×, 1×, 2× and 3×). Maximum shoot yield was observed at 2× macronutrient level. However, the best macronutrient level, which was 1× macronutrient level, resulted in the maximum root and seedling yield. This macronutrient level was applied on the subsequent experiment where seedlings were inoculated on media with various nitratenitrogen (NO3-N) to ammonium-nitrogen (NH4-N) ratios. Seedling yield was the highest at NO3-N/NH4-N ratio of 2.0 while Root to Shoot ratio (R/S) was the highest at NO3-N/NH4-N ratio of 5.0. NO3-N/NH4-N ratio significantly affected Water Content (WC) of seedlings and the response to NO3-N/NH4-N ratio was similar to R/S, correlating better root development to higher WC. By altering NO3-N/NH4-N ratio, plant quality in terms of yield and organ development could be encouraged. Science Publications 2015 Article PeerReviewed text en http://eprints.intimal.edu.my/640/1/Effect%20of%20In%20Vitro%20Nitrogen%20Nutrition%20to%20Phalaenopsis%20deliciosa.pdf Choong, Chieh Wean and Lim, Chin Hao and Xiong, Zherui and Choong, Siew Mee (2015) Effect of In Vitro Nitrogen Nutrition to Phalaenopsis deliciosa Seedling Growth. OnLine Journal of Biological Sciences, 16 (1). pp. 17-25. ISSN 1608-4217 http://thescipub.com/abstract/10.3844/ojbsci.2016.17.25 |
| spellingShingle | TP Chemical technology Choong, Chieh Wean Lim, Chin Hao Xiong, Zherui Choong, Siew Mee Effect of In Vitro Nitrogen Nutrition to Phalaenopsis deliciosa Seedling Growth |
| title | Effect of In Vitro Nitrogen Nutrition to Phalaenopsis deliciosa Seedling Growth |
| title_full | Effect of In Vitro Nitrogen Nutrition to Phalaenopsis deliciosa Seedling Growth |
| title_fullStr | Effect of In Vitro Nitrogen Nutrition to Phalaenopsis deliciosa Seedling Growth |
| title_full_unstemmed | Effect of In Vitro Nitrogen Nutrition to Phalaenopsis deliciosa Seedling Growth |
| title_short | Effect of In Vitro Nitrogen Nutrition to Phalaenopsis deliciosa Seedling Growth |
| title_sort | effect of in vitro nitrogen nutrition to phalaenopsis deliciosa seedling growth |
| topic | TP Chemical technology |
| url | http://eprints.intimal.edu.my/640/ http://eprints.intimal.edu.my/640/ http://eprints.intimal.edu.my/640/1/Effect%20of%20In%20Vitro%20Nitrogen%20Nutrition%20to%20Phalaenopsis%20deliciosa.pdf |