Effect of In Vitro Nitrogen Nutrition to Phalaenopsis deliciosa Seedling Growth
In a continuous effort to conserve Phalaenopsis species, the optimization of a previously designed defined medium was carried out to understand the effect of in vitro nitrogen nutrition on Phalaenopsis deliciosa Rchb.f. In order not to alter the nutrient balance, macronutrient level instead of s...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Science Publications
2015
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| Subjects: | |
| Online Access: | http://eprints.intimal.edu.my/640/ http://eprints.intimal.edu.my/640/1/Effect%20of%20In%20Vitro%20Nitrogen%20Nutrition%20to%20Phalaenopsis%20deliciosa.pdf |
| Summary: | In a continuous effort to conserve Phalaenopsis species, the
optimization of a previously designed defined medium was carried out to
understand the effect of in vitro nitrogen nutrition on Phalaenopsis
deliciosa Rchb.f. In order not to alter the nutrient balance, macronutrient
level instead of solely Nitrogen (N) was adjusted. Seedlings were obtained
from in vitro germination and randomly inoculated on media with various
macronutrient levels (0.3×, 0.5×, 1×, 2× and 3×). Maximum shoot yield
was observed at 2× macronutrient level. However, the best macronutrient
level, which was 1× macronutrient level, resulted in the maximum root and
seedling yield. This macronutrient level was applied on the subsequent
experiment where seedlings were inoculated on media with various nitratenitrogen
(NO3-N) to ammonium-nitrogen (NH4-N) ratios. Seedling yield
was the highest at NO3-N/NH4-N ratio of 2.0 while Root to Shoot ratio
(R/S) was the highest at NO3-N/NH4-N ratio of 5.0. NO3-N/NH4-N ratio
significantly affected Water Content (WC) of seedlings and the response to
NO3-N/NH4-N ratio was similar to R/S, correlating better root development
to higher WC. By altering NO3-N/NH4-N ratio, plant quality in terms of
yield and organ development could be encouraged. |
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