Tailoring the nitrogen sources of bacterial culture to enhance methyl tert-butyl ether degradation
Four methyl tert-butyl ether (MTBE)-degrading bacteria were isolated from a processing plant in Malaysia. Based on 16S rDNA sequences, the four isolates were identified as Exiguobacterium profundum P1M-2, Bacillus megaterium P1M-11, Alishewanella sp. P2A-12 and Pseudomonas mendocina P2M-8. Each of t...
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| Format: | Article |
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Springer Berlin Heidelberg
2015
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| Online Access: | http://eprints.intimal.edu.my/534/ |
| _version_ | 1848766498904997888 |
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| author | Ainon, Hamzah Wong, Kok Kee |
| author_facet | Ainon, Hamzah Wong, Kok Kee |
| author_sort | Ainon, Hamzah |
| building | INTI Institutional Repository |
| collection | Online Access |
| description | Four methyl tert-butyl ether (MTBE)-degrading bacteria were isolated from a processing plant in Malaysia. Based on 16S rDNA sequences, the four isolates were identified as Exiguobacterium profundum P1M-2, Bacillus megaterium P1M-11, Alishewanella sp. P2A-12 and Pseudomonas mendocina P2M-8. Each of the isolates obtained optimum growth using a different source of nitrogen (0.1- 0.03 % yeast or peptone) and all four isolates were able to biodegrade 92.05-99.98 % of MTBE within seven days. Amongst the four isolates, the highest percentage of MTBE degradation was achieved using B. megaterium P1M-11. The highest growth on tert-butyl alcohol (TBA), tert-amyl alcohol (TAA) and 2-hydroxyisobutyric acid (2-HIBA) was also observed in B. megaterium P1M-11. This study suggests MTBE degradation by each of the bacteria can be enhanced by choosing the right nitrogen source. Furthermore, the ability of B. megaterium P1M-11 to grow on primary metabolites of MTBE and other structurally related ethers suggests the secretion of diverse degradative enzymes, making this isolate a good candidate to be applied in MTBE bioremediation strategies. |
| first_indexed | 2025-11-14T11:52:06Z |
| format | Article |
| id | intimal-534 |
| institution | INTI International University |
| institution_category | Local University |
| last_indexed | 2025-11-14T11:52:06Z |
| publishDate | 2015 |
| publisher | Springer Berlin Heidelberg |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | intimal-5342016-10-25T06:26:05Z http://eprints.intimal.edu.my/534/ Tailoring the nitrogen sources of bacterial culture to enhance methyl tert-butyl ether degradation Ainon, Hamzah Wong, Kok Kee TP Chemical technology Four methyl tert-butyl ether (MTBE)-degrading bacteria were isolated from a processing plant in Malaysia. Based on 16S rDNA sequences, the four isolates were identified as Exiguobacterium profundum P1M-2, Bacillus megaterium P1M-11, Alishewanella sp. P2A-12 and Pseudomonas mendocina P2M-8. Each of the isolates obtained optimum growth using a different source of nitrogen (0.1- 0.03 % yeast or peptone) and all four isolates were able to biodegrade 92.05-99.98 % of MTBE within seven days. Amongst the four isolates, the highest percentage of MTBE degradation was achieved using B. megaterium P1M-11. The highest growth on tert-butyl alcohol (TBA), tert-amyl alcohol (TAA) and 2-hydroxyisobutyric acid (2-HIBA) was also observed in B. megaterium P1M-11. This study suggests MTBE degradation by each of the bacteria can be enhanced by choosing the right nitrogen source. Furthermore, the ability of B. megaterium P1M-11 to grow on primary metabolites of MTBE and other structurally related ethers suggests the secretion of diverse degradative enzymes, making this isolate a good candidate to be applied in MTBE bioremediation strategies. Springer Berlin Heidelberg 2015 Article PeerReviewed Ainon, Hamzah and Wong, Kok Kee (2015) Tailoring the nitrogen sources of bacterial culture to enhance methyl tert-butyl ether degradation. Annals of Microbiology, 65 (2). pp. 1131-1136. ISSN 1869-2044 http://link.springer.com/article/10.1007%2Fs13213-014-0960-6 |
| spellingShingle | TP Chemical technology Ainon, Hamzah Wong, Kok Kee Tailoring the nitrogen sources of bacterial culture to enhance methyl tert-butyl ether degradation |
| title | Tailoring the nitrogen sources of bacterial culture to enhance methyl tert-butyl ether degradation |
| title_full | Tailoring the nitrogen sources of bacterial culture to enhance methyl tert-butyl ether degradation |
| title_fullStr | Tailoring the nitrogen sources of bacterial culture to enhance methyl tert-butyl ether degradation |
| title_full_unstemmed | Tailoring the nitrogen sources of bacterial culture to enhance methyl tert-butyl ether degradation |
| title_short | Tailoring the nitrogen sources of bacterial culture to enhance methyl tert-butyl ether degradation |
| title_sort | tailoring the nitrogen sources of bacterial culture to enhance methyl tert-butyl ether degradation |
| topic | TP Chemical technology |
| url | http://eprints.intimal.edu.my/534/ http://eprints.intimal.edu.my/534/ |