Isolation of Bacteriophages Specific for Lactobacillus casei ATCC 393 Strain 03 and Streptococcus mutans ATCC 35668 from Human Saliva

Streptococcus mutans and Lactobacillus casei are common microflora of the human oral cavity. Bacteriophages are important to ensure controlled microbial population in the oral cavity. Hence, isolation of the bacteriophages specific for S. mutans ATCC 35668 and L. casei ATCC 393 strain 03 from human...

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Main Author: Lim, Jia Wen
Format: Thesis
Language:English
Published: 2015
Subjects:
Online Access:http://eprints.intimal.edu.my/437/
http://eprints.intimal.edu.my/437/1/BBTEI%20-%2079.pdf
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author Lim, Jia Wen
author_facet Lim, Jia Wen
author_sort Lim, Jia Wen
building INTI Institutional Repository
collection Online Access
description Streptococcus mutans and Lactobacillus casei are common microflora of the human oral cavity. Bacteriophages are important to ensure controlled microbial population in the oral cavity. Hence, isolation of the bacteriophages specific for S. mutans ATCC 35668 and L. casei ATCC 393 strain 03 from human saliva samples were done using the Double Agar Overlay Plaque Assay. Ten individuals aged 18-25 years old from INTI International University willingly provided the saliva samples for the isolation of bacteriophages of the mentioned bacteria. The saliva from all the individuals were collected at week 2, week 6 and week 9 of August 2014 session to study the effect of different stress level, diet, sleeping hours and oral hygiene practices to the presence of the bacteriophages. However, no bacteriophage specific for S. mutans ATCC 35668 and L. casei ATCC 393 strain 03 were isolated from the study.
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institution INTI International University
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language English
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publishDate 2015
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spelling intimal-4372016-08-01T06:38:23Z http://eprints.intimal.edu.my/437/ Isolation of Bacteriophages Specific for Lactobacillus casei ATCC 393 Strain 03 and Streptococcus mutans ATCC 35668 from Human Saliva Lim, Jia Wen TP Chemical technology Streptococcus mutans and Lactobacillus casei are common microflora of the human oral cavity. Bacteriophages are important to ensure controlled microbial population in the oral cavity. Hence, isolation of the bacteriophages specific for S. mutans ATCC 35668 and L. casei ATCC 393 strain 03 from human saliva samples were done using the Double Agar Overlay Plaque Assay. Ten individuals aged 18-25 years old from INTI International University willingly provided the saliva samples for the isolation of bacteriophages of the mentioned bacteria. The saliva from all the individuals were collected at week 2, week 6 and week 9 of August 2014 session to study the effect of different stress level, diet, sleeping hours and oral hygiene practices to the presence of the bacteriophages. However, no bacteriophage specific for S. mutans ATCC 35668 and L. casei ATCC 393 strain 03 were isolated from the study. 2015 Thesis NonPeerReviewed text en http://eprints.intimal.edu.my/437/1/BBTEI%20-%2079.pdf Lim, Jia Wen (2015) Isolation of Bacteriophages Specific for Lactobacillus casei ATCC 393 Strain 03 and Streptococcus mutans ATCC 35668 from Human Saliva. Other thesis, INTI International University.
spellingShingle TP Chemical technology
Lim, Jia Wen
Isolation of Bacteriophages Specific for Lactobacillus casei ATCC 393 Strain 03 and Streptococcus mutans ATCC 35668 from Human Saliva
title Isolation of Bacteriophages Specific for Lactobacillus casei ATCC 393 Strain 03 and Streptococcus mutans ATCC 35668 from Human Saliva
title_full Isolation of Bacteriophages Specific for Lactobacillus casei ATCC 393 Strain 03 and Streptococcus mutans ATCC 35668 from Human Saliva
title_fullStr Isolation of Bacteriophages Specific for Lactobacillus casei ATCC 393 Strain 03 and Streptococcus mutans ATCC 35668 from Human Saliva
title_full_unstemmed Isolation of Bacteriophages Specific for Lactobacillus casei ATCC 393 Strain 03 and Streptococcus mutans ATCC 35668 from Human Saliva
title_short Isolation of Bacteriophages Specific for Lactobacillus casei ATCC 393 Strain 03 and Streptococcus mutans ATCC 35668 from Human Saliva
title_sort isolation of bacteriophages specific for lactobacillus casei atcc 393 strain 03 and streptococcus mutans atcc 35668 from human saliva
topic TP Chemical technology
url http://eprints.intimal.edu.my/437/
http://eprints.intimal.edu.my/437/1/BBTEI%20-%2079.pdf