Comparing matK and rbcL Sequences Among Jasminum grandiflorum Jasminum sambac AND Jasminum Nitidum
DNA barcodes are used to assign samples of unknown origin to specific taxonomic groups i.e., short DNA sequences (600-800bp) of a standard segment for species identification. In my study, I used the matK and rbcL barcode sequences to identify the Jasminum grandiflorum, Jasminum sambac and Jasminum n...
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| Format: | Thesis |
| Language: | English |
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2015
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| Online Access: | http://eprints.intimal.edu.my/427/ http://eprints.intimal.edu.my/427/1/BBTEI%20-%2069.pdf |
| _version_ | 1848766473770631168 |
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| author | Lam, Chi Yan |
| author_facet | Lam, Chi Yan |
| author_sort | Lam, Chi Yan |
| building | INTI Institutional Repository |
| collection | Online Access |
| description | DNA barcodes are used to assign samples of unknown origin to specific taxonomic groups i.e., short DNA sequences (600-800bp) of a standard segment for species identification. In my study, I used the matK and rbcL barcode sequences to identify the Jasminum grandiflorum, Jasminum sambac and Jasminum nitidum. In these species, Edwards’s method was able to provide of sufficient quality and quantity of DNA for PCR. In my results, the matK gene showed ease of amplification compared to rbcL gene. The second trial of marK with diluted DNA samples show higher success rate. For species discrimination, the neighbor-joining tree was used to construct phylogenetic trees based on T92 model as it was the best lit sequence model based on mwK and rbcL gene. Based on the maiK gene, J, grandiflorum is more closely related to J. sambac (Distance= 0.010) then J. nitidum (Distance 0.020); while based on the rbcL gene. J. sambac is more closely related to J. nitidum (Distance= 0.007) then J. grandiflorum (Distance= 0.018). Although the tree topology based on maiK and rbcL genes were different, the individuals of the three species grouped together. Hence, J. sambac, J. grandiflorum and J. nitidum can be identified using marK and rbcL gene. Further research to identify and compare with other Jasminum species is needed. |
| first_indexed | 2025-11-14T11:51:43Z |
| format | Thesis |
| id | intimal-427 |
| institution | INTI International University |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T11:51:43Z |
| publishDate | 2015 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | intimal-4272016-08-01T05:57:21Z http://eprints.intimal.edu.my/427/ Comparing matK and rbcL Sequences Among Jasminum grandiflorum Jasminum sambac AND Jasminum Nitidum Lam, Chi Yan TP Chemical technology DNA barcodes are used to assign samples of unknown origin to specific taxonomic groups i.e., short DNA sequences (600-800bp) of a standard segment for species identification. In my study, I used the matK and rbcL barcode sequences to identify the Jasminum grandiflorum, Jasminum sambac and Jasminum nitidum. In these species, Edwards’s method was able to provide of sufficient quality and quantity of DNA for PCR. In my results, the matK gene showed ease of amplification compared to rbcL gene. The second trial of marK with diluted DNA samples show higher success rate. For species discrimination, the neighbor-joining tree was used to construct phylogenetic trees based on T92 model as it was the best lit sequence model based on mwK and rbcL gene. Based on the maiK gene, J, grandiflorum is more closely related to J. sambac (Distance= 0.010) then J. nitidum (Distance 0.020); while based on the rbcL gene. J. sambac is more closely related to J. nitidum (Distance= 0.007) then J. grandiflorum (Distance= 0.018). Although the tree topology based on maiK and rbcL genes were different, the individuals of the three species grouped together. Hence, J. sambac, J. grandiflorum and J. nitidum can be identified using marK and rbcL gene. Further research to identify and compare with other Jasminum species is needed. 2015 Thesis NonPeerReviewed text en http://eprints.intimal.edu.my/427/1/BBTEI%20-%2069.pdf Lam, Chi Yan (2015) Comparing matK and rbcL Sequences Among Jasminum grandiflorum Jasminum sambac AND Jasminum Nitidum. Other thesis, INTI International University. |
| spellingShingle | TP Chemical technology Lam, Chi Yan Comparing matK and rbcL Sequences Among Jasminum grandiflorum Jasminum sambac AND Jasminum Nitidum |
| title | Comparing matK and rbcL Sequences Among Jasminum grandiflorum Jasminum sambac AND Jasminum Nitidum |
| title_full | Comparing matK and rbcL Sequences Among Jasminum grandiflorum Jasminum sambac AND Jasminum Nitidum |
| title_fullStr | Comparing matK and rbcL Sequences Among Jasminum grandiflorum Jasminum sambac AND Jasminum Nitidum |
| title_full_unstemmed | Comparing matK and rbcL Sequences Among Jasminum grandiflorum Jasminum sambac AND Jasminum Nitidum |
| title_short | Comparing matK and rbcL Sequences Among Jasminum grandiflorum Jasminum sambac AND Jasminum Nitidum |
| title_sort | comparing matk and rbcl sequences among jasminum grandiflorum jasminum sambac and jasminum nitidum |
| topic | TP Chemical technology |
| url | http://eprints.intimal.edu.my/427/ http://eprints.intimal.edu.my/427/1/BBTEI%20-%2069.pdf |